单细胞RNA测序技术在视网膜中的研究进展

doi:10.6040/j.issn.1673-3770.0.2023.222

Abstract

Abstract: The retina is a heterogeneous tissue. This intricate structure is composed of various types of cells with distinct transcriptomes. The traditional bulk RNA sequencing has quantified the global transcriptome of retinal cells. In recent years, single-cell RNA sequencing(scRNA-seq)methods have enabled profiling the transcriptome of retinal cells at a single-cell resolution, improving our understanding of cell classification, physiology, and developmental patterns of the retina. Currently, scRNA-seq is mainly focused on exploring the mechanism of retinal diseases. This review provides a comprehensive overview of the utilization of scRNA-seq in retinal research. It encompasses the delineation of cell subtypes, identification of specific markers, insights into retinal disease pathogenesis, developmental patterns, interspecies and interregional retinal tissue comparisons, as well as a concise introduction to scRNA-seq methodologies and prospective advancements in the field.

Key words: Single-cell RNA sequencing, Transcriptome, Heterogeneity, Retina, Retinopathy

CLC Number:

R774.1

YANG Songyue, ZHANG Meixia. Advances in single-cell RNA sequencing in the retina[J].Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(1): 162-170.

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