JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY)

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Age-related spiral ganglion neuron damages and hearing loss in senescence accelerated mice

LIU Qiang-he1, LUO Xiang-lin1, GENG Wan-ping1, CHEN Chen2, LEI Xun1, LIU Fang-xian1, DENG Ming1   

  1. 1. Department of Otorhinolaryngology & Head and Neck Surgery, Affiliated Hospital of Guilin Medical College, Guilin 541001, China; 2. Prince Henry′s Institute of Medical Research, Department of
    Physiology of Monash University, Victoria 3168, Australia
  • Received:2008-03-07 Revised:2008-04-18 Online:2008-06-16 Published:2008-06-16
  • Contact: LIU Qiang-he

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Abstract: To determine age-related spiral ganglion neuron damages and hearing loss in the senescence accelerated mice. MethodsThe auditory thresholds and the morphological changes of the spiral ganglion neurons were studied in senescence accelerated mouse/prone 1 (SAMP 1) mice of 1, 3, 5, 7 and 9 months. Normal aging senescence accelerated mouse/resistance 1 (SAMR 1) mice served as the control group. 8?kHz threshold of auditory brain-stem response was determined, transmission electron microscopy and terminal deoxynucleotidyl transferase(TdT) dUTP nick end labeling(TUNEL) were used to observe the auditory function changes, the morphological changes and the immunohistochemical changes. ResultsAuditory function: Compared with the SAMR 1 mice, SAMP 1 mice of 7 or 9 months developed a progressive hearing loss at 8kHz，which showed an age-related significant increase(P＜0.05). Morphological observation: The SAMR 1 mice had no apoptosis in the spiral ganglion neurons (SGNs), while the SAMP 1 mice of 7 or 9 months developed a significant apoptosis in the SGNs. TUNEL observation: Negative immunohistochemical reaction for TUNEL staining was found in the SGNs in the SAMR 1 mice of 7 months, and a positive reaction was found in the nucleolus of the part of the SGNs of the SAMP 1 mice of 7 months. The image analysis showed that the percentage of positive SGNs of the SAMP 1 mice of 7 months was significantly higher than that of the SAMR 1 mice of 7 months. ConclusionsSAMP 1 mice can be used for investigating mechanism of auditory-function-aging and as a pertinent animal model of presbyacousis.

Key words: Threshold of the auditory brain stem response, Spiral ganglion neuron, Senescence accelerated mouse, Apoptosis

CLC Number: 

  • R764.35
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