JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2016, Vol. 30 ›› Issue (3): 93-95.doi: 10.6040/j.issn.1673-3770.0.2015.517

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Effect of rh-bFGF on the level of glutamic acid in retinal tissue of rabbits with ischemia-reperfusion injury.

SHAO Hongchao, GE Yanran   

  1. Department of Ophthalmology, North China University of Sciense and Technology, Tangshan 063000, Hebei, China
  • Received:2015-12-07 Online:2016-06-16 Published:2016-06-16

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Abstract: Objective To explore the effect of recombinant human basic fibroblast growth factor(rh-bFGF)on the level of glutamic acid in retinal tissue of rabbits with ischemia-reperfusion injury. Methods Sixty-six white rabbits were divided into group A［retinal ischemia-reperfusion injury(RIRI)group, n=30］, group B(RIRI+rh-bFGF, n=30)and group C(sham-operation group, n=6). Groups A and B were also assigned into 5 subgroups respectively 6, 12, 24, 48 and 72 h after ischemia-reperfusion injury, 6 rabbits in each group. The same dose of normal saline and rh-bFGF solution were respectively injected into the vitreous cavity of rabbits in groups A and B at the early stage of molding. The rabbits were killed and their eyeballs were taken out at five time points after molding. Puncture of anterior chamber was given in group C, and no other operations were given. The rabbits in group C were killed and their eyeballs were taken out. The changes of retinal pathomorphism were observed, and the contents of glutamic acid were detected. Results In group A, the retina was characterized by high edema, disordered levels and loose cell structure. Vacuole degeneration was present in retinal ganglion cells(RGC)and and the number of ganglion cells decreased. In group B, the retinal structure changes were similar to that in A group, but the damage was significantly milder than that in group A. In group C, the levels of retina were clear, the cells were arranged in parallel and retinal morphology was reguLar. RGC was reguLarly arranged in a single layer, and no vacuole degeneration occurred. 6, 12, 24, 48 and 72 h after ischemia-reperfusion injury, the contents of glutamic acid in groups A and B were dramatically higher than that in group C(P<0.01). When compared with group A, the contents of glutamic acid in group B decreased notably at different time points(P<0.01). Conclusion rh-bFGF has a protective effect on retinal injury in rabbits, and its mechanism may be related to the inhibition of cell apoptosis and decreased content of glutamic acid.

Key words: Recombinant human basic fibroblast growth factor, Retina, Ischemia-reperfusion injury, Glutamic acid

CLC Number: 

  • R774.1
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