Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2024, Vol. 38 ›› Issue (2): 7-12.doi: 10.6040/j.issn.1673-3770.0.2023.233

• Original Article • Previous Articles     Next Articles

Expression and significance of CXCL10 and IFN-γ in antrochoanal polyp

ZHANG Yongcai1, ZHANG Jiang1, LU Hui1, XING Kexin1, WU Jingfang1, XUE Gang2, LI Yanping2, LIU Yanbin2   

  1. 1. Basic Medical College, Hebei North University, Zhangjiakou 075000, Hebei, China2. The First Clinical College of Hebei North University, Zhangjiakou 075000, Hebei, China
  • Online:2024-03-20 Published:2024-03-29

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Abstract: Objective To investigate the expression and significance of inflammatory cells,CXC chemokine ligand 10(CXCL10), and interferon-γ(IFN-γ)in the antrochoanal polyp(ACP). Methods A total of 58 samples were collected from the First Affiliated Hospital of Hebei North University between October 2015 and July 2020, including 40 cases of ACP and 18 case of maxillary sinus cysts. Preoperative serum and surgical samples were collected. Paraffin embedding and HE staining were performed on surgical specimens, and the number and percentage of various inflammatory cells were counted. The epithelial hyperplasia of patients with ACP was comprehensively scored. The expression of CXCL10 and IFN-γ in ACP and control groups was observed by immunohistochemical staining. The enzyme-linked immunosorbent assay detected the protein concentration of serum CXCL10 in two groups, and the correlation between protein concentration and inflammatory cells in peripheral blood of the two groups was analyzed. Results HE staining revealed obvious epithelial remodeling and heterogeneity with inflammatory cell infiltration, primarily neutrophils, lymphocytes, and plasma cells in group ACP. A positive correlation was found between ACP neutrophils and lymphocytes with the analysis by routine blood examination. Immunohistochemistry showed that CXCL10 and IFN-γ in the experimental group were strongly positive compared with those in the control group. The concentration of CXCL10 was(32.15±4.60)pg/mL vs(12.11± 3.54)pg/mL in ACP and control groups as determined by ELISA(P<0.05). Conclusion The expression of CXCL10 and IFN-γ were significantly upregulated in the ACP group, which may be related to non-type Ⅱ inflammation, especially Th1 inflammatory factors.

Key words: Antrochoanal polyp, Epithelial remodeling, Endotype, CXC chemokine ligand 10, Interferon-γ

CLC Number: 

  • R765.4
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