JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2014, Vol. 28 ›› Issue (1): 1-5.doi: 10.6040/j.issn.1673-3770.0.2013.237

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Establishment of sensorineural deafness model in C57 mice by cisplatin

HE Jing-chun1, RUAN Qing-wei2, HAN Miao-miao1, JIN Bin1, LI Ke-yong1, DONG Pin1   

  1. 1.Department of Otolaryngology ＆ Head and Neck Surgery,Shanghai First People′s Hospital, Shanghai Jiao Tong University，Shanghai 200080, China; 2. Central Laboratory,  Huadong Hospital Affiliated to Fudan University, Shanghai 200040, China
  • Received:2013-08-08 Online:2014-02-16 Published:2014-02-16

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Abstract:

Objective   To establish a cisplatin-induced sensorineural deafness model in C57 mice. Methods   C57BL/6J mice were divided randomly into four groups：group A (5μL normal saline injected into the round window niche), group B (5μLcisplatin (1mg/mL) injected into the round window niche), group C (10μL cisplatin (1mg/mL) injected daily into middle ear cavity for 5 days), and group D ( cisplatin (6mg/kg) injected daily into the abdominal cavity for 5 days). 10 dB increase or above in the reaction threshold of  whole octave band auditory brainstem response (ABR) was to define the decline of the hearing capacity. The stereocilia and cuticular plate of hair cells stained with fluorescent phalloidin were observed. Results   In group A,C and D, the whole octave band ABR reaction thresholds showed no difference before and after the administration (P>0.05). However, the reaction threshold were all elevated 48h after the administration in B group (P<0.05). Meanwhile, in group B, outer hair cell (OHC) lesions were more severe than those of inner hair cell (IHC), and the hair cells lesions aggravated gradually from the base to apex of chochlea. Conclusion   The administration of cisplatin through the window niche is the effective method to establish sensorineural deafness model in C57 mice.

Key words: Ototoxicity, Mice, Animal model, Cisplatin

CLC Number: 

  • R764
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