Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2018, Vol. 32 ›› Issue (6): 30-37.doi: 10.6040/j.issn.1673-3770.0.2018.021

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Effect of MicroRNA-24 on the proliferation, migration, invasion, and apoptosis of LSCC cells

XU Li1, WANG Zhiyi1, CHEN Wei1, XUE Fei1, ZHANG Yong1, CHENG You1, JI Junfeng1, CUI Pengcheng2, JIANG Manjie1   

  1. Department of Otolaryngology Head and Neck Surgery, Tangdu Hospital, Air Force Military Medical University, Xian 710038, Shaanxi, China
  • Published:2018-11-29

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Abstract: Objective The effect of miR-24 on the proliferation, migration, invasion, and apoptosis of LSCC cells was examined. Methods After constructing Hep-2 and AMC-HN-8 cells to overexpress miR-24 by cell transfection, the proliferation of Hep-2 and AMC-HN-8 cells was detected by MTT assay and a cloning assay. A scratch test and Transwell assay were conducted to detect cell migration and invasion abilities, and the effect on apoptosis was analyzed by using flow cytometry analysis. Results After cell transfection, qRT-PCR confirmed upregulation of miR-24 in Hep-2/miR-24 and AMC-HN-8/miR-24 cells. MTT and colony formation assays showed that upregulation of miR-24 significantly reduced the proliferation of LSCC cells. Additionally, the capacity for colony formation in Hep-2/miR-24 and AMC-HN-8/miR-24 cells was significantly reduced compared to that of control cells. The scratch test and Transwell assay showed that the migration and invasion ability of miR-24 were significantly reduced. Flow cytometry showed that overexpression of miR-24 significantly enhanced apoptosis in LSCC cells. These results suggest that miR-24 inhibits the proliferation, invasion, and metastasis and increases the apoptosis of LSCC cells. Conclusion Abnormal expression of miR-24 is closely related to properties of LSCC cells. miR-24 inhibits the proliferation and migration and enhances the apoptosis of LSCC cells.

Key words: Laryngeal squamous carcinoma, MicroRNA-24, Inhibition, Proliferation, Invasion, Apoptosis

CLC Number: 

  • R739.65
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