Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2020, Vol. 34 ›› Issue (6): 72-76.doi: 10.6040/j.issn.1673-3770.0.2019.618

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Expression and clinical significance of CPS1 in laryngeal squamous cell carcinoma

FAN Yiyan1,2, ZHANG Xiaolin1, LIU Xiuzhen3, YIN Jingjing1, YUAN Jin1, WANG Yanfei1, CHEN Jun1   

  1. 1. Otorhinolaryngology & Head and Neck Surgery, Binzhou Medical University Hospital, Binzhou 256603, Shandong, China;
    2. Department of Otolaryngology, Shengli Oilfield Central Hospital, Dongying 257000, Shandong, China;
    3. Clinical Medical Laboratory, Binzhou Medical University Hospital, Binzhou 256603, Shandong, China
  • Published:2021-01-11

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Abstract: Objective To study the expression levels of carbamoyl phosphate synthase I(CPS1)in laryngeal squamous cell carcinoma and adjacent non-cancerous tissues and reveal its possible correlation with clinical parameters, and explore the clinical significance of CPS1 in the disease. Methods The real-time quantitative polymerase chain reaction(RT-qPCR)was used to measure the expression of CPS1 mRNA in laryngeal squamous cell carcinoma and adjacent tissues. Western blotting and immunohistochemistry were used to detect expression levels of the CPS1 protein and its distribution in laryngeal squamous cell carcinoma tissues. Results The results of RT-qPCR and western blot analysis showed that the mRNA and protein expression levels of CPS1 in laryngeal squamous cell carcinoma were significantly higher than that in adjacent tissues(P<0.01). Immunohistochemistry showed the same trend in CPS1 protein levels. The expression of CPS1 was closely related to the degree of tumor differentiation, clinical stage, and lymph node metastasis(P<0.05), but not to the patients' age, gender, smoking history, drinking history, and tumor site(P>0.05). Conclusion The expression of CPS1 in laryngeal squamous cell carcinoma is significantly higher than in the adjacent tissues, and is closely related to clinical progression. These results suggest that CPS1 may play a role in the occurrence and metastasis of laryngeal cancer, and is a diagnostic indicator for laryngeal cancer.

Key words: Carbamyl phosphate synthase 1, Laryngeal squamous cell carcinoma, Clinical progress

CLC Number: 

  • R739.65
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