Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2022, Vol. 36 ›› Issue (2): 32-39.doi: 10.6040/j.issn.1673-3770.0.2021.119

Previous Articles     Next Articles

CHD1L promotes proliferation, invasion and metastasis of laryngeal squamous cell carcinoma cells by EMT

LIU Yong1, YUAN Cunli1, CAO Hui1, ZHENG Chengcai1, CHAO Fang2, XU Fenglei3   

  1. 1.Department of Otolaryngology, Rizhao Central Hospital, Rizhao 276800, Shandong, China;
    2.First Operating Room, Rizhao People's Hospital, Rizhao 276826, Shandong, China;
    3. Department of Otolaryngology, Shandong Provincial Hospital, Jinan 250022, Shandong, China
  • Published:2022-04-15

PDF (PC)

236

Abstract: Objective To investigate the mechanism of chromodomain helicase DNA binding protein 1-like gene(CHD1L )in the proliferation, invasion and metastasis of laryngeal squamous cell carcinoma cells and to provide molecular targets for the treatment of laryngeal squamous cell carcinoma(LSCC). Methods Immunohistochemistry was conducted to measure the expression of CHD1L, E-cadherin and Vimentin in 60 pairs of LSCC and its adjacent normal mucosa. The expression of CHD1L in human bronchial epithelial cell(HBE)and human laryngeal epithelioid carcinoma cell(HEp-2)was detected by Western blotting. CHD1L in HEp-2 cells was knocked out, and Western blotting was used to detect the expression of CHD1L, E-cadherin and Vimentin in HEp-2 cells. The proliferation of cells in each group was detected by CCK-8 kit, and the invasion of cells in each group was detected by Transwell invasion test. Results Immunohistochemical results showed that the positive expression rate of CHD1L in laryngeal squamous cell carcinoma tissue was significantly higher than that of normal paracaner mucosa tissues. In addition, the expression of CHD1L was positively correlated with the degree of differentiation of laryngeal squamous cell carcinoma, the lymph node metastasis, the clinical stage and the expression of Vimentin, but negatively correlated with the expression of E-cadherin(P<0.05). The results of Western blotting showed that the expression of CHD1L in HEp-2 cell was significantly higher than that in HBE cell. CHD1L in HEp-2 cells was knocked down by interfering plasmid in vitro. The proliferation and invasion ability of the HEp-2 cells with CHD1L knockdown in vitro were significantly decreased, while the expression of E-cadherin was significantly increased and the expression of Vimentin was also significantly decreased. Conclusion The high expression of CHD1L in LSCC was closely related to pathological grades, clinical stage and risk of lymph node metastasis of LSCC, suggesting that CHD1L may play an oncogene role in LSCC; CHD1L could promote the proliferation, invasion and metastasis of LSCC cells by promoting the EMT of LSCC cells.

Key words: Laryngeal squamous cell carcinoma, Chromodomain helicase/ATPase DNA binding protein 1-like gene, Epithelial-mesenchymal transition, Proliferation, Invasion, Metastasis

CLC Number: 

  • R739.65
[1] Siegel RL, Miller KD, Jemal A. Cancer statistics, 2018[J]. CA Cancer J Clin, 2018, 68(1): 7-30. doi:10.3322/caac.21442.
[2] Tang X, Sun Y, Wan G, et al. Knockdown of YAP inhibits growth in Hep-2 laryngeal cancer cells via epithelial-mesenchymal transition and the Wnt/β-catenin pathway[J]. BMC Cancer, 2019, 19(1): 654.doi:10.1186/s12885-019-5832-9.
[3] 蒋宏,王斌全,高泽慧,等.喉癌患者情绪和认知功能改变的研究进展[J].临床耳鼻咽喉头颈外科杂志,2018,32(11):880-882. doi:10.13201/j.issn.1001-1781.2018.11.021. JIANG Hong, WANG Binquan, GAO Zehui, et al. Research progress on the changes of emotional and cognitive functions in patients with laryngeal cancer[J]. Journal of Clinical Otorhinolaryngology Head and Neck Surgery, 2018, 32(11): 880-882. doi:10.13201/j.issn.1001-1781.2018.11.021.
[4] Liu ZH, Zhang Q, Ding YJ, et al. Overexpression of CHD1L is associated with poor survival and aggressive tumor biology in esophageal carcinoma[J]. Oncotarget, 2017, 8(43): 74178-74187.doi:10.18632/oncotarget.18830.
[5] Li SM, Chai Y, Ding YB, et al. CHD1L is associated with poor survival and promotes the proliferation and metastasis of intrahepatic cholangiocarcinoma[J]. Oncol Rep, 2019, 42(2): 657-669.doi:10.3892/or.2019.7174.
[6] Mu QJ, Li HL, Yao Y, et al. Chromodomain helicase/ATPase DNA-binding protein 1-like gene(CHD1L)expression and implications for invasion and metastasis of breast cancer[J]. PLoS One, 2015, 10(11): e0143030. doi:10.1371/journal.pone.0143030.
[7] Nieto MA, Huang RYJ, Jackson RA, et al. Emt: 2016[J]. Cell, 2016, 166(1): 21-45. doi:10.1016/j.cell.2016.06.028.
[8] Su Z, Zhao J, Xian G, et al. CHD1L is a novel independent prognostic factor for gastric cancer[J]. Clin Transl Oncol, 2014, 16(8): 702-707. doi:10.1007/s12094-013-1136-8.
[9] Famokunwa B, Walsted ES, Hull JH. Assessing laryngeal function and hypersensitivity[J]. Pulm Pharmacol Ther, 2019, 56: 108-115. doi:10.1016/j.pupt.2019.04.003.
[10] Al-Rohil RN, Tarasen AJ, Carlson JA, et al. Evaluation of 122 advanced-stage cutaneous squamous cell carcinomas by comprehensive genomic profiling opens the door for new routes to targeted therapies[J]. Cancer, 2016, 122(2): 249-257. doi:10.1002/cncr.29738.
[11] Peyvandi H, Peyvandi AA, Safaei A, et al. Introducing potential key proteins and pathways in human laryngeal cancer: a system biology approach[J]. Iran J Pharm Res, 2018, 17(1): 415-425.
[12] Liu W, Xu J, Zhang C. Prognostic role of chromodomain helicase DNA binding protein 1-like protein in human solid cancers: a meta-analysis[J]. Medicine(Baltimore), 2018, 97(29): e11522.doi:10.1097/md.0000000000011522.
[13] Li Y, He LR, Gao Y, et al. CHD1L contributes to cisplatin resistance by upregulating the ABCB1-NF-κB axis in human non-small-cell lung cancer[J]. Cell Death Dis, 2019, 10(2): 99. doi:10.1038/s41419-019-1371-1.
[14] Su FR, Ding JH, Bo L, et al. Chromodomain helicase/ATPase DNA binding protein 1-like protein expression predicts poor prognosis in nasopharyngeal carcinoma[J]. Exp Ther Med, 2014, 8(6): 1745-1750. doi:10.3892/etm.2014.2017.
[15] Tian F, Xu F, Zhang ZY, et al. Expression of CHD1L in bladder cancer and its influence on prognosis and survival[J]. Tumour Biol, 2013, 34(6): 3687-3690. doi:10.1007/s13277-013-0951-4.
[16] Wang W, Wu J, Fei X, et al. CHD1L promotes cell cycle progression and cell motility by up-regulating MDM2 in breast cancer[J]. Am J Transl Res, 2019,11(3):1581-1592.
[17] He LR, Ma NF, Chen JW, et al. Overexpression of CHD1L is positively associated with metastasis of lung adenocarcinoma and predicts patients poor survival[J]. Oncotarget, 2015, 6(31): 31181-31190.doi:10.18632/oncotarget.5070.
[18] Skrypek N, Goossens S, De Smedt E, et al. Epithelial-to-mesenchymal transition: epigenetic reprogramming driving cellular plasticity[J]. Trends Genet, 2017, 33(12): 943-959.doi:10.1016/j.tig.2017.08.004.
[19] Nakagawa H, Hikiba Y, Hirata Y, et al. Loss of liver E-cadherin induces sclerosing cholangitis and promotes carcinogenesis[J]. PNAS, 2014, 111(3): 1090-1095. doi:10.1073/pnas.1322731111.
[20] Po JW, Roohullah A, Lynch D, et al. Improved ovarian cancer EMT-CTC isolation by immunomagnetic targeting of epithelial EpCAM and mesenchymal N-cadherin[J]. J Circ Biomark, 2018, 7: 184945441878261. doi:10.1177/1849454418782617.
[21] Li T, Xie J, Shen C, et al. Upregulation of long noncoding RNA ZEB1-AS1 promotes tumor metastasis and predicts poor prognosis in hepatocellular carcinoma[J]. Oncogene, 2016, 35(12): 1575-1584.doi:10.1038/onc.2015.223.
[22] Matysiak M, Kapka-Skrzypczak L, Jodowska-Jędrych B, et al. EMT promoting transcription factors as prognostic markers in human breast cancer[J]. Arch Gynecol Obstet, 2017, 295(4): 817-825.doi:10.1007/s00404-017-4304-1.
[23] Mizukoshi K, Okazawa Y, Haeno H, et al. Metastatic seeding of human colon cancer cell clusters expressing the hybrid epithelial/mesenchymal state[J]. Int J Cancer, 2020, 146(9): 2547-2562.doi:10.1002/ijc.32672.
[24] Karlsson MC, Gonzalez SF, Welin J, et al. Epithelial-mesenchymal transition in cancer metastasis through the lymphatic system[J]. Mol Oncol, 2017, 11(7): 781-791. doi:10.1002/1878-0261.12092.
[25] Karamagkiolas S, Giotakis I, Kyrodimos E, et al. Expression of vimentin(VIM)and metastasis-associated 1(MTA1)protein in laryngeal squamous cell carcinoma are associated with prognostic outcome of patients[J]. Am J Otolaryngol, 2019, 40(4): 487-493. doi:10.1016/j.amjoto.2019.04.002.
[26] Mezi S, Chiappetta C, Carletti R, et al. Clinical significance of epithelial-to-mesenchymal transition in laryngeal carcinoma: Its role in the different subsites[J]. Head Neck, 2017, 39(9): 1806-1818.doi:10.1002/hed.24838.
[27] Haga RB, Ridley AJ. Rho GTPases: Regulation and roles in cancer cell biology[J]. Small GTPases, 2016, 7(4): 207-221. doi:10.1080/21541248.2016.1232583.
[28] Liu M, Chen LL, Ma NF, et al. CHD1L promotes lineage reversion of hepatocellular carcinoma through opening chromatin for key developmental transcription factors[J]. Hepatology, 2016, 63(5): 1544-1559. doi:10.1002/hep.28437.
[29] Li FF, Zhang Z, Wang P, et al. ALC1 knockdown enhances cisplatin cytotoxicity of esophageal squamous cell carcinoma cells by inhibition of glycolysis through PI3K/Akt pathway[J]. Life Sci, 2019,232: 116679. doi:10.1016/j.lfs.2019.116679.
[1] CHEN Dongyan, QIAN Ye, WEI Dongmin, LI Wenming, XIA Tongliang, LEI Dapeng, PAN Xinliang. Clinical value of high-frequency ultrasound in the diagnosis of lymph node metastasis in hypopharyngeal squamous cell carcinoma [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(5): 18-23.
[2] YAN Fancheng, JIANG Xian, CHAI Yijie, WANG Haosen, MENG Zhaoyang, WANG Xiaolei, WANG Yanling,. miR-30-5p inhibits retinoblastoma cell proliferation by downregulating FOXG1 expression [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(5): 63-69.
[3] HUANG Danyi, ZHANG Ting,CHEN Jing, ZHANG Wei. Progress of research regarding the role of the epithelial barrier in chronic rhinosinusitis with nasal polyps [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(3): 78-83.
[4] WANG JunxinOverview,SUN YanGuidance. Research progress of miRNA-29b involved in EMT-related signaling pathway regulation [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2021, 35(6): 132-137.
[5] LIU Pan, WANG Chuan, ZHAO Zeqi, WU Mei, XU Jifeng, LI Wei, ZHANG Weiqiang, LIU Wen. Five cases of metastatic clear cell carcinoma of the head and neck: a case report and literature review [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2021, 35(4): 45-50.
[6] LI Jingjing, WU Xinxin, MAO Ning, ZHENG Guibin, MU Yakui, CHU Tongpeng, JIA Chuanliang, ZHENG Haitao, MI Jia, SONG Xicheng. CT-based radiomics nomogram for the preoperative prediction of central lymph node metastases of papillary thyroid carcinoma [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2021, 35(4): 51-59.
[7] WANG Yingying, ZHOU Han, DONG Weida, XING Guangqian, CHEN Zhibin, ZHANG Qingzhao, ZHANG Liqing. Analysis of distant metastasis and prognosis of adenoid cystic carcinoma of the external auditory canal [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2021, 35(1): 1-6.
[8] PANG Zhenwen, HUANG Yufeng, YANG Aifang, ZENG Xianjie. Correlation between Preoperative neutrophil/lymphocyte ratio and lymph node metastasis in patients with laryngeal cancer [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2020, 34(6): 58-62.
[9] FAN Yiyan, ZHANG Xiaolin, LIU Xiuzhen, YIN Jingjing, YUAN Jin, WANG Yanfei, CHEN Jun. Expression and clinical significance of CPS1 in laryngeal squamous cell carcinoma [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2020, 34(6): 72-76.
[10] PEI XueyanOverview,WANG YanGuidance. Progress in the research on the roles of invadopodia and metalloproteinase-14 in tumorigenesis and cancer development [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2020, 34(6): 129-134.
[11] CHEN Haibing, WEI Ya'nan, XU Xiaoquan, CHEN Xi. Prediction of cervical lymph node metastasis in papillary thyroid cancer based on XGBoost artificial intelligence and enhanced computed tomography [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2020, 34(3): 40-45.
[12] XIA Tongliang, WEI Dongmin, QIAN Ye, LI Wenming, JI Wei, PAN Xinliang, LEI Dapeng. Clinical analysis of malignant thyroid tumors with laryngotracheal invasion [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2020, 34(3): 46-51.
[13] LÜ Jingrong, CHEN Chun, MA Yan, XIE Jin. Clinical characteristics and risk factors for differentiated thyroid carcinoma in children [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2020, 34(3): 88-94.
[14] WANG Jiashuo, GUO Xing, YAN Aihui. Giant cyst formation from lymph node metastasis in papillary thyroid cancer: a case study [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2020, 34(3): 111-113.
[15] NING Yudong, CAI Yongcong, SUN Ronghao, JIANG Jian, ZHOU Yuqiu, SHUI Chunyan, WANG Xu, ZHENG Wanghu,. Preliminary analysis of the anterior superior mediastinal lymph node metastasis in thyroid papillary carcinoma [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2020, 34(3): 120-124.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] HE Shou-huan,CHEN Bin,YIN Shan-kai,SU Kai-ming,JIANG Xiao . Morphological changes of the upper airway in OSAHS patients with UPPP
[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(5): 385 -388 .
[2] SONG Xi-cheng,ZHANG Qing-quan,XIA Yong-hong,LIU Lu-yi, YU Lu-xin,WANG Gao,JIANG Xiu-liang . Post-operative ICU monitoring and nursing for patients with OSAHS[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(5): 389 -392 .
[3] XUE Wei-guo,SUN Jie,JIN Zheng,SHI Wen-bin,XIN Lu,LIN Guo-Jing,LI Jia-yun . [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2006, 20(4): 300 -303 .
[4] ZHANG Qing-quan,LI Xin-min,WANG Qiang,WANG Yong-fu . Some experience of the endoscopy treatment of maxillary sinus lesion with the canine tooth fossa approach[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2007, 21(1): 38 -39 .
[5] DONG Pin,LI Xiao-yan,TU Li-qiang,MENG Qing-hong,WANG Sang,XIE Jin,JIANG Yan . Reconstruction for advanced hypopharyngeal carcinoma and laryngeal recurrent carcinoma[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2007, 21(5): 385 -387 .
[6] JIANG Shao-hong,ZHU Yu-hong,WANG Qiang,SONG Xi-cheng . Intractable primary epistaxis: a report of 101 cases[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2007, 21(6): 542 -544 .
[7] . [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(1): 74 -77 .
[8] LEIXun, LIU Qianghe, KONG Zhongyu, XIANG Qiu, GENG WANping, HUANG Hui, DONG Yiyuan, LIU Fangxian. ARelationship between expression of Survivin and radiosensitization effect of EGCG in xenotransplanted nasopharyngeal carcinoma[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2009, 23(1): 6 -9 .
[9] . [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2009, 23(2): 73 -74 .
[10] . [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2010, 24(01): 29 -33 .
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd