Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2024, Vol. 38 ›› Issue (2): 41-51.doi: 10.6040/j.issn.1673-3770.0.2023.217

• Original Article • Previous Articles     Next Articles

Elucidation of the Mongolian medicine Huricha-6 mechanism in treating allergic rhinitis via network pharmacology and animal experiments

SU Riguge, LI Hua, WU Richaifu, HAN Eerdemutu, MENG Yongmei   

  1. College of Mongdian Medicine, Inner Mongolia Medical Vniversity, Hohhot 010110, Inner Mongolia, China
  • Online:2024-03-20 Published:2024-03-29

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Abstract: Objective To explore the possible mechanism of Mongolian medicine Huricha-6 in treating allergic rhinitis,based on network pharmacology and animal experiments. Methods Compounds and targets of Huricha-6 were obtained from the TCMSP database and literature. Allergic rhinitis(AR)-related targets were identified via searches of the OMIM, TTD, Disgenet, and GeneCards databases. Then, the Huricha-6 target and AR target intersection was identified by constructing a "compound-intersection target" network and selecting key compounds. The STRING database was used to draw the protein interaction network to screen core targets, and R language was used to conduct gene ontology(GO)and kyto encyclopedia of genes and genomes(KEGG)pathway analysis on intersection targets. AutoDockVina software was used to verify key compounds and targets by molecular docking. An AR guinea pig model was constructed for Huricha-6 testing. Guinea pigs were treated with Huricha-6 for 6 weeks, then their nasal symptoms were evaluated by behavioral assessment. Nasal mucosa tissue was taken for HE staining to observe lesions, and IL-4 and IFN-γ levels were detected by ELISA. Results Network pharmacology analysis identified 107 possible targets for Huricha-6 relevant to AR treatment, and 30 compounds related to Huricha-6 in the treatment of AR. Quercetin, luteolin, kaolin, isorhamnetin and quinine were the key compounds, and the key target include VEGFA, STAT3, IL-1B, IL-6 and ALB. KEGG pathway enrichment revealed involvement of TNF, IL-17, Toll and HIF-1 pathways. Molecular docking supports the premise that key compounds will exhibit strong binding to core targets. Animal experiments demonstrate that Huricha-6 can effectively reduce nasal symptoms, improve pathological changes in the nasal mucosa, significantly reduce serum IL-4 levels, and increase IFN-γ levels. Conclusion Huricha -6 can ameliorate AR symptoms via interactions with multiple targets that act in multiple pathways, may alleviate inflammatory cell infiltration of nasal mucosal tissue, and alleviate AR symptoms by modulating the inflammatory response.

Key words: Mongolian medicine, Huricha-6, Allergic rhinitis, Network pharmacology, Animal experiments

CLC Number: 

  • R765.21
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