Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2023, Vol. 37 ›› Issue (3): 21-29.doi: 10.6040/j.issn.1673-3770.0.2022.137

• 论著 • Previous Articles    

Effects of high fat diet on allergic rhinitis mice and intestinal flora

WANG Weiyi, SHI Lei, ZHANG Zhiyu, ZHANG Guiling, SHI Guanggang   

  1. Department of Otorhinolaryngology & Head and Neck Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan 250000, Shandong, China
  • Published:2023-05-24

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Abstract: Objective We investigated the effect of high-fat diet(HFD)on the sensitization of allergic rhinitis(AR)mice and change of intestinal flora and its regulatory mechanism. Methods The control(CN), allergic rhinitis model(AR)induced by chicken ovalbumin(OVA), and allergic rhinitis model(HFD-AR)groups exposed to high-fat diet were set up, with seven mice in each group. The times of sneezing and scratching the nose of each mouse were recorded, and the concentration of anti OVA IgE in serum and IL-4 in nasal lavatory were detected by ELISA. The nasal mucosa and lung were sectioned and stained with HE. Additionally, the total DNA of stool samples was extracted to determine the purity and integrity of the genome for 16S rRNA sequencing and data analysis. Results The frequency of nose scratching and sneezing in the AR group was higher than that in the CN group after OVA stimulation. Compared with the AR group, the HFD-AR group exhibited increased nose scratching and sneezing in mice. Compared with the CN group, the level of specific IgE against OVA in serum and the level of IL-4 in the nasal lavage fluid of mice in the HFD-AR and AR groups increased. HFD intervention aggravated eosinophil infiltration in nasal mucosa and inflammatory cell infiltration in lung tissue of AR group mice. The diversity and number of flora in the HFD-AR group were lower than those in the AR group. The intestinal microflora structure of mice in the CN, AR, and HFD-AR groups were isolated, while the sample isolation in the group was small. At the phyla and genus levels, numerous different bacterial populations were observed among the three groups of mice. Conclusion The intervention of high-fat diet aggravates the symptoms and inflammatory infiltration of tissues in OVA-induced AR mice and reduces the diversity and abundance of intestinal flora. The nasal inflammation of mice aggravated by high-fat diet is related to intestinal flora imbalance.

Key words: Allergic rhinitis, High fat diet, Intestinal flora, Inflammation

CLC Number: 

  • R765
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