Table of Content

16 April 2010 Volume 24 Issue 2

  

Articles

Invasion of the CD133-positive cells in FADU cell line

LIANG Hui1,2, PAN Xinliang1, MA Chao3, WANG Qirong2, HAN Fei2, CUI Zhaoyang2

JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2010, 24(2):  5-7. 

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Objective  To investigate the invasion of the CD133-positive cells in FADU cell line. Methods  In our study, Immunomagnetic beads were applied to purify CD133-positive cells in FADU cell line. Invasions of CD133-positive and CD133-negative cells were measured by the Transwell experiment. Results  The number of cells  infiltrating the Transwell membrane in the CD133-positive group (54±2.3) was significantly higher than that in the CD133-negative group (17±1.1). Conclusion  CD133-positive cells in FADU cell line show higher invasive ability.

Clinical features of laryngeal carcinoma in females in the east of Shandong Province

LIU Zhang, SUN Yan, HUA Hui, CHEN Zhijun, LI Wei, SUN Wenhai

JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2010, 24(2):  8-10. 

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Objective  To investigate the clinical features of laryngeal carcinoma in females in the East of Shandong Province. Methods  Twenty-two female patients with laryngeal carcinoma were retrospectively analyzed, including 8 cases of supraglottic carcinoma, 13 cases of glottic carcinoma and 1 case of subglottic carcinoma. According to AJCC（2002）, there were 10 cases in stageⅠ, 2 cases in stageⅡ,4 cases in stage Ⅲ, and 6 cases in stage Ⅳ. Partial laryngectomy was performed in 14 patients and total laryngectomy in 6 patients. 10 patients underwent neck dissection synchronously. Radiotherapy was performed in 10 patients after surgery. And 2 patients only received radiotherapy. The direct method was used to calculate the survival rate. Results  Among the 20 patients who received surgery, 11 were followed up for more than 3 years: 2 were missed and 8 were disease-free survival. The 3-year survival rate was 72.7%. Only 6 patients were followed up for more than 5 years: 1 was missed and 5 were disease-free survived. The 5-year survival rate was 83.3%. The two patients who only received radiotherapy died because of the relapse in 9 months and 1 year after treatment respectively. Conclusion  Laryngeal carcinoma in females has a low incidence. Supraglottic carcinoma is relatively common in female patients. Smoking is an important factor in some females. Multiple primary carcinomas should be focused on in females.

Expression of the KAI1 gene in laryngeal carcinoma and metastasis lymph nodes

JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2010, 24(2):  11-13. 

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Objective  To find out the association between expression of the KAI1 gene and the metastasis of laryngeal carcinoma by determining expression of the KAI1 protein in human laryngeal squamous cell carcinomas and metastasis lymph nodes, and attempt. Methods  Using the immunohistochemical S-P technique, we studied the expression of KAI1 in 40 laryngeal squamous cell carcinoma specimens, 13 metastasis lymph node specimens and 30 carcinoma side normal tissue specimens with monoclonal antibody of KAI1. Correlations of expression of the KAI1 gene to clinicopathologic factors were statistically analyzed. Results  The KAI1-positive rates in carcinoma side normal tissues, laryngeal squamous cell carcinoma and metastasis lymph nodes were 93.33%(28/30) , 62.5%（25/40）and 30.77%（4/13）respectively, and the differences were statistically significant (P＜0.05). Expression of the KAI1 gene was related to the clinical stage (P＜0.05). There was no relationship of the expression of the KAI1 gene to the patients′ gender, the patients′ age, the primary site of tumor, pathological grades and lymphatic metastasis(P＞0.05). Conclusion  There is a significant reduce in the expression of the KAI1 gene in metastasis lymph nodes compared with laryngeal squamous cell carcinomas. The KAI1 gene may play a role in the metastasis of laryngeal carcinomas.

Diagnosis of parapharyngeal space schwannoma and  selection of surgical approach

ZHANG Jian-wei1, NI Ze1, GAO Xia2

JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2010, 24(2):  14-16. 

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Objective  To investigate the diagnosis of parapharyngeal space schwannoma and the selection of surgical approach modes. Methods  14 medical records of patients with parapharyngeal space schwannoma were retrospectively analyzed. According to preoperative CT and MRI, surgical approach was determined depending on the distance between the tumor and tonsillar capsule and the tumor main location: with a distance between the tumor and tonsillar capsule ≤1cm, and the main tumor breaking into the mouth, 4 cases via the mouth diameter road; with the distance >1cm, and the tumor projected to the outside of the main body, 10 cases via the neck diameter road. There was no significant relationship between surgical approach and the tumor size. Results  All patients had received successful surgery, and were followed up for 6 months to 6 years without recurrence. Conclusions  Neck CT, MRI, and DSA are good means of the diagnosis of parapharyngeal space schwannoma, and the selection of surgical approach depends on the distance between the tumor and tonsillar capsule and tumor main location. No matter through which pathway, there are no serious complications.

Dynamic expression of neurotrophic factor Ⅳ in the rat model  of facial nerve injury

QIN Chen, WANG Haibo，LI Jianfeng, HAN Yuechen, LIU Wenwen, LU Sumei

JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2010, 24(2):  17. 

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Objective  To evaluate dynamic expression changes of neurotrophin-4 (NT-4）in the rat facial nerve, vibrissae muscle and facial nucleus in response to facial nerve injury.  Methods    A rat model of facial nerve injury was established. Subsequently, all facial nerves, the vibrissae muscle and pons were taken out on days 1, 3, 7, 14, and 21 after injury. Expression of NT-4 mRNA was detected by RT-PCR. Expression of NT-4 at the protein level was simultaneously determined by Western blot and immunohistochemisty. Results  Expression of NT-4 was detected in the facial nerve, vibrissae muscle and facial nucleus. Expression of NT-4 was elevated and reached the peak at 14th day in the facial nerve and facial nucleus, whereas reduced in the muscle.  Conclusions   Expression of NT-4 was increased in the facial nerve and nucleus and decreased in the vibrissae muscle after facial nerve injury. These indicate that expression of NT-4 in the nerve tissue could be closely related to the injury in the facial nerve.

Application of Otorhinlaryngology,The Second Clinical College of Jinan University,Shenzhen People's Hospital,Shenzhen 518020,Guangdong,China

KE Zhaoyang， GONG Taogen， YANG Mingbao

JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2010, 24(2):  21-23. 

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Objective  To explore the value and experience of the application of bone pate from self mastoid cortex in the micro-otosurgery． Methods  Bone pate from self mastoid cortex was applied in 46 cases of chronic suppurative otitis media and cholesteatomatons from 2007 to 2009 in our department． 19 patients were treated with radical mastoidectomy, the other 27 with tympanoplasty and mastoidectomy． During surgery，bone pate from mastoid cortex was collected by drilling using a large cutting burr， and then was used to fill the attic cell or mastoidale, and obliterate the antrum. Afterwards，the temporalis fascia was used to overlap the surface of the bone pate． Results  All patients achieved a small， dry and healthy mastoid cavity. The average time of dry ear was 6 weeks; the rate of dry ear in the three months was 95.7%（44/46）; the shape of the eardrum was normal, and no suction pocket was found in patients treated with tympanoplasty． Conclusion  Bone pate from self mastoid cortex with less rejection, easy to obtain and shape, is a kind of good repairing material in the micro-otosurgery．

Auricular traumatic injuries(163 cases)

cui xin hua,wang qi rong,han fei,gu lin tao

JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2010, 24(2):  24. 

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Objective To reduce the deformity rate of injured ears and to investigate the treatment of auricular traumatic injuries. Methods  All patients were treated with different operative methods, and then received negative pressure drainage and local heparinization after operation, according to various severity of injuries. Results  The results of all cases after treatment were satisfied. Conclusion  Negative pressure drainage and heparinization contribute to the recovery of the auricular traumatic injuries and a decrease in infection and deformity rates.

 Expressions of SOCS3 and Eotaxin-2 in allergic rhinitis

WANG Xinlong1， ZHAO Jiandong2

JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2010, 24(2):  26-29. 

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Objective  To investigate expressions of SOCS3 and Eotaxin-2 in allergic rhinitis(AR)． Methods  Twenty cases of allergic rhinitis nasal mucosa tissues served as the experimental group and fifteen cases of deflection of nasal septum nasal mucosa tissues as the control group．Immunohistochemisty was performed to detect expressions of SOCS3 and Eotaxin-2．The relationship between SOCS3 and Eotaxin-2 expressions was analyzed．Results  SOCS3 and Eotaxin-2 expressions were increased in the AR patients．There were positive staining in inflammatory cells, vascular endothelial cells，epithelial cells and glandular epithelium cells．Compared with the control group，expressions of SOCS3(0.2401±0.01463) and of Eotaxin-2(0.2398±0.01423) significantly higher than those in the control group(P<0.01). The SOCS3 expression was closely related to Eotaxin-2 expression(r=0.951,P<0.01).      Conclusion  Increased expressions of SOCS3 and Eotaxin-2 in allergic rhinitis are well evidenced. SOCS3 shows positive correlation with Eotaxin-2.

Clinical assessment and endoscopic surgery in chronic sinusitis without nasal polyps of adult patients

YUAN Xiaopei1, WANG Jianying2, XING Zhimin1, YU Lisheng1

JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2010, 24(2):  30-34. 

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Objective  To discuss the basic characteristics and effective treatment of chronic sinusitis without nasal polyps (CRSsNP) of adult patients in China. Methods  78 cases were scored by the quantitative assessment: CT scans by Lund-Mackay scoring system, nasal endoscopy by Lund-Kennedy score, and symptoms severity score by visual analog scale (VAS scale ). Combined with the chief complaint and objective score, we analyzed the abnormal anatomy of the nasal cavity which cause the disease and carried out individual endoscopic surgery. After surgery, patients were followed up for one year, and then were scored again and statistically analyzed. Results  Ostiomeatal complex and maxillary sinus were involved completely. Pathologic examination showed that plasma cells and lymphocytes were the main infiltrating cells. The total response rate was 100%: 53 patients were recovered and 25 patients improved. The average epithelization time was five weeks. The occurrence rate of the lesions was significantly higher in the front sinuses than in the latter sinuses. In patients with0-3 grades, there was no significant difference in the VAS score before and after surgery, while in patients with other grades a significant difference in the VAS score and the Lund-Kennedy score. Conclusion  CRSsNP of adults in China has its own features: multi-local anatomical abnormalities may play an important  role in the pathogenesis, and ostiomeatal complex and the maxillary sinus are the target areas; bacterial infection may be more important than allergy; the pathogenesis of unilateral and bilateral CRSsNP may be different; EPOS2007 guide provide a more objective assessment method which can fully reflect the disease and its treatment; Surgical treatment of CRSsNP plays an important role and endoscopic individual rhinoplasty is an effective method.

Lamellar coronal sectional anatomy for endoscopic sphenoidotomy

ZHAO Zhenhua1, WANG Qirong1, LIU Shuwei2, HAN Fei1, GU Lintao1, Li Xiao1, Chen Zhipeng1

JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2010, 24(2):  35-37. 

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Objective  To provide lamellar sectional anatomy data of coronal for endoscopic sphenoidotomy.  Methods  A digital freezing milling technique was used to obtain continuous sectional data of coronal as thin as 0.1mm.of 10 cadaver heads(20 sides) without organic lesions. The sphenoid sinus and some surrounding structures were observed on typical and continuous coronal sectional images.  Results  75% of the sphenoid sinus was sellae type,  only 15% of the septum located in the median sagittal section,  60% of the optical nerve canal and 75% of the internal carotid bulge on the area of the lateral sphenoid sinus. Conclusion  The lamellar coronal sectional image obtained from digital freezing milling technique offers a better understanding of the complex anatomic structures and provides sectional anatomy data for sphenoidotomy.

Application of radiofrequency electrical ion comprehensive treatment  instrument in the post-operative patients with chronic nosesinusitis

XUE Zhangwei, FANG Wenxu, HU Weiqun

JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2010, 24(2):  38. 

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Objective   To observe the clinical efficacy of radiofrequency electrical ion comprehensive treatment instrument in the treatment of common postoperative complications of chronic nose-sinusitis. Methods  108 patients with chronic nose-sinusitis were randomly divided into group A and group B. In group A (RF group), the granulation tissue, adhesions organizations and atresia of sinus ostium stenosis were cleaned up by radiofrequency electrical ion comprehensive treatment instrument; in group B (conventional group), cavities were dealed with by endoscopic sinus surgery instruments under general endoscope. Clinical response and cure rates were analyzed. Results  The cure rate was similar between group A and group B(59.26% vs 51.85%); the response rate in group A (92.59%) was significantly higher than that in group B (83.33%); the rate of epithelialization in group A (57.41%) was higher than that in group B (37.04%)  during the 2-4 month period, the results were statistically significant (P＜0.01) . Conclusion  Application of RF ion instrument in comprehensive treatment of postoperative complications of chronic nose-sinusitis has significant effect, shortening the postoperative follow-up schedule and effectively controling the recurrence of complications.

Two kinds of surgical treatments for bilateral vocal cord paralysis

PENG Feng, MO Songping, FU Shinan, YANG Liming

JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2010, 24(2):  55-56. 

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Objective  To explore the optimal treatment for bilateral vocal cord paralysis by comparing arytenoidectomy through the neck with the endoscopic CO2 laser arytenoidectomy. Methods  13 cases of bilateral vocal cord paralysis were retrospectively reviewed. 7 received the arytenoidectomy through the neck and 6 received the endoscopic CO2 laser arytenoidectomy.  All 13 cases received preoperative tracheotomy and postoperative follow-up from 6 months to 2 years. Results  3 out of 7 patients receiving the arytenoidectomy through the neck and 4 out of 6 patients receiving the endoscopic CO2 laser arytenoidectomy gained extubation smoothly. Conclusion  The two kinds of surgical treatment methods have their own advantages and disadvantages.

Electrocoagulation tonsillectomy (a report of 180 cases)

ZHU Jiangcai, JIN Bin, LIN Xiao, ZHANG Jia, LI Keyong, SUN Yajing, DONG Pin

JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2010, 24(2):  57. 

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Objective  To investigate the feasibility of electrocoagulation tonsillectomy. Methods  180 patients who underwent electrocoagulation tonsillectomy (method A), and 35 patients who underwent coblation tonsillectomy (method B) were retrospectively studied. Results  The bleeding volume measured during operation was 5-50mL (10mL on average) in method A group, and 5-200mL (50mL on average) in method B group. There was one patient with postoperative bleeding in tonsillar fossa in method B group and no patients with postoperative infection in neither groups. Conclusion  Electrocoagulation tonsillectomy is effective and safe, with less volume of bleeding during the surgery. At the same time, there is a decrease in the chance of postoperative bleeding and no increase in the incidence of postoperative complications. Electrocoagulation tonsillectomy is feasible.

Application of rod-lens and conventional bronchoscopy in tracheobronchial foreign body extraction

HONG Yuming， LIN Rongzhi， LIANG Zhenyuan， LIN Poshui

JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2010, 24(2):  59-61. 

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Objective  To improve the diagnosis and treatment of tracheobronchial foreign bodies and lower the complication rate by comparing the applications of rod-lens and conventional bronchoscopy in tracheobronchial foreign body extraction. Methods  The diagnosis, anaesthesia methods,operation methods and therapeutic effect of 206 cases of foreign bodies were reviewed. Rod-lens coupled with grasping forceps sheaths tracheobronchial foreign body extraction was compared with conventional bronchoscopic foreign body extraction. Results  Patients  extracted by rod-lens coupled with grasping forceps were all primarily successful. 10 patients extracted by conventional bronchoscope were operated on for the second time. In bronchoscopic foreign body extraction, 8 cases of laryngospasm and 12 cases of bronchiospasm were found  during operation, and there were one case of mediastinal emphysema, 2 cases of pneumothorax, 4 cases of subcutaneous emphysema, 2 cases of heart failure and 5 tracheotomy after operation. No death and serious complication occurred. Conclusions  Rod-lens coupled with grasping forceps sheaths for extraction of tracheobronchial foreign bodies has its own advantanges of visualization and amplification, avoiding the blindness of conventional bronchoscopic foreign body extraction and making the surgery safer and more successful.

Multi-purpose electric ion operating apparatus treatment for benignvocal fold hyperplastic diseases under video rhinolaryngoscope

HE Zhongyang, PENG Shuxin, QIN Hongkang, TANG Liang

JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY). 2010, 24(2):  62. 

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Objective  To evaluate the applicability and effectiveness of multi-purpose electric ion operating apparatus in the treatment of benign vocal fold hyperplastic diseases under video rhinolaryngoscope. Methods  GX-Ⅲtype multi-purpose electric ion operating apparatus was used in treatment of 82 cases of benign vocal fold hyperplastic diseases (25 cases of single side and 57 cases of bilateral lesions) under Olympus ENT-VT video laryngoscope. 82 patients who underwent surgery for excision of benign vocal fold lesions under local anesthesia were reviewed: 36 with polyps, 33 with nodules, 7 with cysts and 6 with angeiomas. Results  The surgery was completed smoothly in all patients with a recovery rate of 92.7 %（76∕88）and an improvement rate of 7. 3 %(6∕88). No complications were found. The mean follow-up period was 3.2 months (range, 3-6 months).  Conclusions  Multi-purpose electric ion operating apparatus used to manage benign vocal fold hyperplastic diseases under video rhinolaryngoscope has simple procedure, less pain and mild damage to normal tissues. It offers a new, simple and effective treatment option for vocal fold masses removal.