鼻咽癌EGFL7的表达与肿瘤侵袭转移的关系

doi:10.6040/j.issn.1673-3770.0.2015.307

摘要/Abstract

摘要： 目的观察表皮生长因子样结构域7(EGFL7)在鼻咽癌中的表达,并了解其与微血管密度(MVD)、淋巴管密度(LVD)的相关性,探讨其与鼻咽癌侵袭转移的关系及其可能的机制.方法采用免疫组化法检测40例鼻咽癌组织(实验组)及20例鼻咽炎(对照组)中的EGFL7的表达;用CD31抗体及D2-40抗体分别检测实验组及对照组的MVD及LVD,并分析EGFL7的表达与肿瘤分级及MVD、LVD三者的相关性.结果实验组与对照组均有EGFL7的表达,但实验组中EGFL7高表达率高于对照组(P<0.05);实验组中EGFL7的表达与MVD呈正相关,但与LVD相关性不明显;实验组中有淋巴结转移的LVD明显高于无淋巴结转移的(P<0.05);实验组中EGFL7的表达与患者性别、年龄、EB病毒抗体无相关性(P>0.05),与临床分期及有无淋巴结转移有关(P<0.05).结论 EGFL7并不像VEGF家族对血管、淋巴管均有双重作用,它仅表达于血管内皮细胞及鼻咽癌细胞中.EGFL7与鼻咽癌的生长和侵袭转移关系密切,它可能成为反映鼻咽癌转移及预后的标志物之一.

关键词: 微血管密度, 淋巴管密度, VEGF家族, 表皮生长因子样结构域7, 鼻咽癌

Abstract: Objective To study the expression of epidermal growth factor-like domain 7(EGFL7) in nasopharyngeal carcinoma(NPC), and to investigate its correlation with microvessel density(MVD) and lymphatic vessel density(LVD) and its relationship with the invasion and metastasis of NPC and its possible mechanism. Methods The immunohisto chemical method was used to detect EGFL7 expression of 40 cases of nasopharyngeal carcinoma and 20 cases of nasopharyngitis; MVD and LVD were detected by antibody CD31 and D2-40 antibody for immunohistochemistry in the experimental and control groups, and to analyze the correlation of EGFL7 expression and tumor grade and MVD and LVD. Results The experimental group and the control group were of EGFL7 expression, but in the experimental group EGFL7 expression rate was higher than that of control group (P<0.05); EGFL7 expression and MVD was positively correlated in the experimental group, but LVD did not; in the experimental group, lymph node metastasis of LVD was significantly higher than those without lymph node metastasis of (P<0.05); in the experimental group, EGFL7 expression had no correlation with patient gender, age and EB virus antibody (P>0.05) but had some correlation with clinical stage and lymph node metastasis(P<0.05). Conclusion EGFL7 has no dual function on blood vessels and lymphatic vessels, it only expresses in endothelial cells and nasopharyngeal carcinoma cells. EGFL7 is closely associated with the growth and invasion and metastasis of nasopharyngeal carcinoma (NPC), it may become one of the markers reacting nasopharyngeal carcinoma metastasis and prognosis.

Key words: Nasopharyngeal carcinoma, Epidermal growth factor-like domain 7, VEGF family, Lymphatic vessel density, Micro vessel density

中图分类号:

R739.63

赵燕恋, 卢永田, 杨继红, 张娟, 苗芳芳, 李洁萍. 鼻咽癌EGFL7的表达与肿瘤侵袭转移的关系[J]. 山东大学耳鼻喉眼学报, 2015, 29(6): 31-35.

ZHAO Yanlian, LU Yongtian, YANG Jihong, ZHANG Juan, MIAO Fangfang, LI Jieping. Relationship between the expression of EGFL7 in nasopharyngeal carcinoma and the invasion and metastasis of tumor[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2015, 29(6): 31-35.

参考文献

[1] 李骥,王胜资,王纾宜,等.鼻咽癌淋巴管和微血管同期检测与预后[J].临床耳鼻咽喉头颈外科杂志,2010,24(6):253-256. LI Ji, WANG Shengzi, WANG Shuyi, et al. Prognostic value of synchronized detection of microvascular and lymphvessel density in nasopharyngeal carcinoma[J]. J Clin Otorhinolaryngol Head Neck Surgery, 24(6):253-256.
[2] Saad R S, Lindner J, Liu Y, et al. Lymphatic vessel density as prognostic marker in esophageal ade2 nocarcinoma[J]. Am J Clin Pathol, 2009, 131(1):92-98.
[3] 桑娜,王宽松,李景和.Egfl7与肿瘤研究新进展[J].现代生物医学进展,2009,9(23):4556-4559. SANG Na, WANG Kuansong, LI Jinghe. EGFL7 and advances in carcinoma research[J]. Prog Mod Biomed, 2009, 9(23):4556-4559.
[4] Schmidt M, De maziere A, Smyczek T, et al. The role of Egfl7 in vascular morphogenesis[J]. Novartis Found Symp, 2007, 283(1):18-28.
[5] 李京佳,林相国,许涛,等.VEGF家族及其在肿瘤生长中作用的研究[J].现代生物医学进展,2012,12(4):777-780. LI Jingjia, LIN Xiangguo, XU Tao, et al. VEGF and the role of VEGF in tumor growth[J]. Prog Mod Biomed, 2012, 12(4):777-780.
[6] 李文光,张娟,陈永平,等.鼻咽癌VEGF-C和Flt-4的表达与淋巴管生成及淋巴结转移的相关性[J].中国耳鼻咽喉颅底外科杂志,2013,19(3):196-203. LI Wenguang, ZHANG Juan, CHEN Yongping, et al. Expression of VEGF-C and its receptor Flt-4 in nasopharyngeal carcinoma and their relationship with lymph node metastasis[J]. Chin J Otorhinolaryngol Skull Base Surgery, 2013, 19(3):196-203.
[7] 王益民,吴奎,诸禹平,等.EGFL7和VEGF在肾透明细胞癌血管生成及转移中的相关性研究及临床意义[J].临床泌尿外科杂志, 2011,26(10):732-736. WANG Yimin, WU Kui, ZHU Yuping, et al. The correlative research of EGFL7, VEGF Expression in clear cell renal cell carcinoma with angiogenesis and metastasis[J]. J Clin Urol, 2013, 19(3):196-203.
[8] 高永超,杨新明,李仕晟,等.EGFL7在喉癌组织中的表达及临床意义[J].临床耳鼻咽喉头颈外科杂志,2013,27(3):147-150. GAO Yongchao, YANG Xinming, LI Shisheng, et al. The study on clinical significance of the expression of EGFL7 in laryngeal squamous cell carcinoma[J]. J Clin Otorhinolaryngol Head Neck Surgery, 2013, 27(3):147-150.
[9] Chatni S S, Thankappan K. Lymph node status cannot be ignored in survival analysis of laryngeal cancer[J]. Arch Otolaryngol Head Neck Surg, 2008, 134(7):786-787.
[10] 王晓侠,姚小宝,嵇宪生,等.EGFL7基因过度表达与喉癌分化、转移及预后的关系[J].华西医学,2011,26(5):710-713. WANG Xiaoxia, YAO Xiaobao, JI Xiansheng, et al. Association of EGFL7 mRNA overexpression with differentiation, metastasis and prognosis of laryngocarcinoma[J]. West China Med J, 2011, 26(5):710-713.
[11] 王启广,曹友德,刘琼,等. EGFL7在胃癌组织中的表达研究[J].湖南师范大学学报,2013,3(1):53-56. WANG Qiguang, CAO Youde, LIU Qiong, et al. The expression of EGFL7 in gastric carcinoma[J]. J Hunan Normal Univ (Med Sci), 2013, 3(1):53-56.
[12] 刘志勇,欧阳忠,邹小明.EGFL7基因沉默对裸鼠胃癌血管生成的影响[J].贵阳医学院学报,2010,35(3):259-264. LIU Zhiyong, OUYANG Zhong, ZOU Xiaoming. Effect of epidermal growth factor-like domain 7 gene silencing on angiogenesis of stomach carcinoma in nude mice[J]. J Guiyang Med Coll, 2010, 35(3):259-264.
[13] 刘志勇,欧阳忠,邹小明.EGFL7基因沉默对裸鼠乳腺癌血管生成的影响[J]. 国际免疫学杂志,2010, 33(6):476-480. LIU Zhiyong, OUYANG Zhong, ZOU Xiaoming. Effect of RNA interference targeting at EGFL7 on angiogenesis of breast carcinoma in nude mice[J]. Inter J Immunol, 2010, 33(6):476-480.
[14] 刘凤娟,张春玲.非小细胞肺癌Egfl7表达与肿瘤转移及预后的关系[J].齐鲁医学杂志,2012,27(3):104-106. LIU Fengjuan, ZHANG Chunling. Relationship between expression of egfl7 and both metastatis and prognosis in non-small-cell lung cancer[J]. Med J Qilu, 2012, 27(3):104-106.
[15] 李学军,黄纯海,李萃,等.EGFL7在人脑胶质瘤中的表达及其意义[J].肿瘤防治研究,2011,38(2):148-152. LI Xuejun, HUANG Chunhai, LI Cui, et al. Expression of EGFL7 in human gliomas and its correlation with glioma grade[J]. Cancer Res Prev Treat, 2011, 38(2):148-152.
[16] Huang C H, Li X J, Zhou Y Z, et al. Expression and clinical significance of EGFL7 in malignant glioma[J]. J Cancer Res Clin Oncol, 2010, 136(11):1737-1743.

相关文章 15

[1]	林小雪,林葆睿,李佩珊,卢标清. 电子鼻咽镜联合窄带成像技术在鼻咽癌中医辨证中的应用[J]. 山东大学耳鼻喉眼学报, 2026, 40(3): 40-46.
[2]	王盛,李银丹,杨金姬. microRNA在鼻咽癌中的作用及其研究进展[J]. 山东大学耳鼻喉眼学报, 2025, 39(5): 125-131.
[3]	邱前辉,肖旭平,杨钦泰,叶菁,邓泽义,王德生,谭国林,蒋卫红,卢永田,唐隽,石照辉,邓晓聪,刘遗斌,王跃武,段传志,杜德坤,白小欣,陈文伙,莫立根,蔡楚伟,曾鹏,何旭英,杨一梅,赵洲洋,陈健龙,赵充,林志雄,李先明,李曙平,陈冬平,陈勇,黄莹,陈春燕,韩非,黄理金,瞿申红. 鼻咽癌治疗后并发颈动脉爆裂综合征的临床处理专家共识[J]. 山东大学耳鼻喉眼学报, 2025, 39(4): 1-18.
[4]	王思权,朱洪申,张晓斌,赵洲洋,马跃,杨一梅,黄理金. 放射治疗后鼻咽癌患者单侧颈内动脉栓塞术后脑卒中及颅神经麻痹的相关因素分析[J]. 山东大学耳鼻喉眼学报, 2025, 39(4): 19-25.
[5]	黄巧,任毅,侯涛,廖行伟,朱子昂,詹晓琳,刘盈,尹时华. 鼻咽癌组织中EphB2表达及与临床病理特征的相关性[J]. 山东大学耳鼻喉眼学报, 2025, 39(4): 26-30.
[6]	孙春晓,王文晴,岳田,刘济生. 高低累积顺铂剂量同步放化疗治疗鼻咽癌的疗效分析[J]. 山东大学耳鼻喉眼学报, 2025, 39(4): 31-41.
[7]	徐飞,朱光熹,王可心. 基于决策树算法构建鼻咽癌患者放疗后发生放射性口腔黏膜炎风险的预测模型[J]. 山东大学耳鼻喉眼学报, 2025, 39(4): 42-48.
[8]	王再兴,唐志元,李定波,石照辉,曾宪海,张秋航. 鼻咽癌放疗后肿瘤复发及颅底骨坏死引起颈内动脉破裂的治疗方案[J]. 山东大学耳鼻喉眼学报, 2025, 39(4): 49-58.
[9]	孙芳,谢楚波,邱前辉. 营养指标对鼻咽癌放射性颅底坏死患者创面修复影响的回顾性分析[J]. 山东大学耳鼻喉眼学报, 2025, 39(4): 59-68.
[10]	朱瑞楷,吴家荣,孙芳,谢楚波,邱前辉. 基于计算机断层扫描血管造影术评估鼻咽癌放疗后引起颈内动脉狭窄状况及其影响因素的研究[J]. 山东大学耳鼻喉眼学报, 2025, 39(4): 77-84.
[11]	覃德波,薛建成,杨文月,胡兵,陈涛,俞艳萍,孟庆国,孙焕吉,苗北平,卢永田. 鼻咽癌诊疗变革:生物标志物与鼻内镜手术协同推进早期治疗发展[J]. 山东大学耳鼻喉眼学报, 2025, 39(4): 85-92.
[12]	吴家荣,邱前辉. 颅底筋膜组织在早期复发性鼻咽癌内镜手术中的临床意义[J]. 山东大学耳鼻喉眼学报, 2025, 39(4): 108-113.
[13]	杨鸣,刘雪霞,张华. m6A识别蛋白IGF2BPs家族在头颈肿瘤中的研究进展[J]. 山东大学耳鼻喉眼学报, 2025, 39(3): 153-161.
[14]	吴敏,李正阳,孟杰,叶惠平. 程序性细胞死亡的分子机制和其在鼻咽癌中的作用[J]. 山东大学耳鼻喉眼学报, 2025, 39(2): 152-157.
[15]	张茂华,魏日富,朱忠寿,刘平,高尚,李慧凤. LncRNA PCAT-1对鼻咽癌细胞生物学行为及化疗敏感性的影响[J]. 山东大学耳鼻喉眼学报, 2025, 39(1): 68-76.

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed