人头颈鳞癌细胞中CDH13的表达及其甲基化状态研究

doi:10.6040/j.issn.1673-3770.0.2016.362

山东大学耳鼻喉眼学报 ›› 2017, Vol. 31 ›› Issue (4): 60-63.doi: 10.6040/j.issn.1673-3770.0.2016.362

人头颈鳞癌细胞中CDH13的表达及其甲基化状态研究

赵锦成,石颖,张颖,贾占红,马新,张京秋,吴再军,王宇

陆军总医院二六三临床部耳鼻咽喉头颈外科, 北京 101149

收稿日期:2016-08-17 出版日期:2017-08-16 发布日期:2017-08-16
通讯作者: 王宇. E-mail:wangyumike@126.com

Expression and methylation patterns of CDH13 in human head and neck squamous carcinoma cells.

ZHAO Jincheng, SHI Ying, ZHANG Ying, JIA Zhanhong, MA Xin, ZHANG Jingqiu, WU Zaijun, WANG Yu

Department of Otolaryngology-Head and Neck Surgery, 263 Clinical Department of General Army Hospital, Beijing 101149, China

Received:2016-08-17 Online:2017-08-16 Published:2017-08-16

摘要/Abstract

摘要： 目的研究抑癌基因H-钙黏素(CDH13)在人头颈部鳞癌细胞系SCC10A和PCI-37B中的表达水平及其启动子区域的甲基化状态。方法定量 PCR和Western blotting检测CDH13在头颈鳞癌细胞系SCC10A和PCI-37B及两种正常组织中的mRNA和蛋白表达变化,并通过甲基化特异性PCR和硫化测序检测CDH13基因启动子区域的甲基化情况。结果与正常组织比较,头颈鳞癌细胞SCC10A和PCI-37B中CDH13的mRNA和蛋白表达水平均降低。SCC10A和PCI-37B细胞中CDH13基因甲基化水平高于口腔黏膜正常组织,人头颈鳞癌细胞系中CDH13基因启动子区域呈高甲基化状态。结论 CDH13启动子区域高甲基化可能是导致人头颈鳞癌中CDH13表达沉默的重要因素,但其影响肿瘤发生发展的具体机制仍需进一步探究。

关键词: 头颈鳞癌, 表达水平, CDH13, 甲基化

Abstract: Objective To explore the expression levels and methylation patterns of the tumor-suppressor gene H-Cadherin 13(CDH13)in the head and neck squamous cell carcinoma(HNSCC)SCC10A and PCI-37B cell lines. Methods We selected 2 normal oral mucosa tissues as the control group, and used quantitative PCR and western blotting, respectively, to detect the relative mRNA and protein expression levels of CDH13 in SCC10A and PCI-37B cells. We then analyzed the methylation status of the promoter region of CDH13 using methylation-specific PCR(MSP)and bisulfite sequencing PCR(BSP). Results The mRNA and protein expression levels of CDH13 in SCC10A and PCI-37B were significantly reduced compared to the control group. Methylation of the CDH13 promoter was considerably higher in SCC10A and PCI-37B cells than in normal tissues; BSP confirmed this finding. Conclusion The high methylation levels of the CDH13 gene promoter may play an important role in down-regulating the expression of CDH13 in HNSCC cells, but further study is required to determine its influence on HNSCC.

Key words: Head and neck squamous cell carcinoma, Expression, Methylation, CDH13

中图分类号:

R739.91

赵锦成,石颖,张颖,贾占红,马新,张京秋,吴再军,王宇. 人头颈鳞癌细胞中CDH13的表达及其甲基化状态研究[J]. 山东大学耳鼻喉眼学报, 2017, 31(4): 60-63.

ZHAO Jincheng, SHI Ying, ZHANG Ying, JIA Zhanhong, MA Xin, ZHANG Jingqiu, WU Zaijun, WANG Yu. Expression and methylation patterns of CDH13 in human head and neck squamous carcinoma cells.[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(4): 60-63.

参考文献

[1] Schmitz S, Machiels JP. Molecular biology of squamous cell carcinoma of the head and neck relevance and therapeutic implications[J]. Expert Rev Anticancer Ther, 2010, 10(9):1471-1484.
[2] Rezende TM, De Souza Freire M, Franco OL. Head and neck cancer: Proteomic advances and biomarker achievements[J]. Cancer, 2010, 116(21):4914-4925.
[3] Chen CY, Huang CC, Cheng JT, et al. The clincopathological significance of p53 and p21 expression in squamous cell carcinoma of hypopharyngealcancer[J]. Cancer Lett, 2003, 201(2):217-223.
[4] Poetsch M, Lorenz G, Kleist B. Detection of new PTEN/MMAC1 mutation in head and neck squamous cell carcinoma with loss ofchromosome10[J]. Cancer Genet Cytogenet, 2002, 132(1):20-24.
[5] Coppoia D, Szabo M, Boulware D, et al. Correlation of osteopontin protein expression and pathological stage across a wide variety of tumor histologies[J]. Clin Cancer Res, 2004, 10(1):184-190.
[6] Wu W, Tang X, Hu W, et al. Identification and validation of metastasis-associated proteins in head and neck cancer cell lines by two-dimensional electrophoresis and mass spectrometry[J]. Clin Exp Metastasis, 2002, 19(4):319-326.
[7] Carvalho AL, Jeronimo C, Kim MM, et al. Evaluation of promoter hypermethylation detection in body fluids as a screening /diagnosis tool for head and neck squamous cell carcinoma[J]. Clin Cancer Res, 2008, 14(1):97-107.
[8] Sanchez-Cespedes M, Esteller M, Wu L, et al. Gene promoter hypermethylation in tumors and serum of head and neck cancer patients[J]. Cancer Res, 2000, 60(4):892-895.
[9] Kaur J, Demokan S, Tripathi SC. Promoter hypermethylation in Indian primary oral squamous cell carcinoma[J]. Int J Cancer, 2010, 127(10):2367-2373.
[10] Wu JC, Santi DV. Kinetic and catalytic mechanism of HhaI methyltransferase[J]. J Biol Chem, 1987, 262(10):4778-4786.
[11] 陈浩明, 梅晓春. 表观遗传现象[M] //薛京伦. 表观遗传学. 上海:上海科学技术出版社, 2006: 61-62.
[12] Shen Z, Chen X, Li Q, et al. Elevated methylation of CMTM3 promoter in the male laryngeal squamous cell carcinoma patients[J]. Clin Biochem, 2016, 49(16-17):1278-1282.
[13] Lim Y, Wan Y, Vagenas D, et al. Salivary DNA methylation panel to diagnose HPV-positive and HPV-negative head and neck cancers[J]. BMC Cancer, 2016, 16(1):749.
[14] Gu X, Boldrup L, Coates PJ, et al. Epigenetic regulation of OAS2 shows disease-specific DNA methylation profiles at individual CpG sites[J]. Sci Rep, 2016, 6:32579.
[15] Chen LH, Hsu WL, Tseng YJ, et al. Involvement of DNMT 3B promotes epithelial-mesenchymal transition and gene expression profile of invasive head and neck squamous cell carcinomas cell lines[J]. BMC Cancer, 2016, 16:431.
[16] Scarpa M, Scarpa M, Castagliuolo I, et al. Aberrant gene methylation in non-neoplastic mucosa as a predictive marker of ulcerative colitis-associated CRC[J]. Oncotarget, 2016, 7(9):10322-10331.
[17] Sheng Y, Wang H, Liu D, et al. Methylation of tumor suppressor gene CDH13 and SHP1 promoters and their epigenetic regulation by the UHRF1/PRMT5 complex in endometrial carcinoma[J]. Gynecol Oncol, 2016, 140(1):145-151.
[18] Huang KT, Mikeska T, Li J, et al. Assessment of DNA methylation profiling and copy number variation as indications of clonal relationship in ipsilateral and contralateral breast cancers to distinguish recurrent breast cancer from a second primary tumour[J]. BMC Cancer, 2015, 15:669.
[19] Kitamoto A, Kitamoto T, Nakamura T, et al. CDH13 polymorphisms are associated with adiponectin levels and metabolic syndrome traits independently of visceral fat mass[J]. J Atheroscler Thromb, 2016, 23(3):309-319.
[20] Cho CH, Lee HJ, Woo HG, et al. CDH13 and HCRTR2 may be associated with hypersomnia symptom of bipolar depression:a genome-wide functional enrichment pathway analysis[J]. Psychiatry Investig, 2015, 12(3):402-407.

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人头颈鳞癌细胞中CDH13的表达及其甲基化状态研究

Expression and methylation patterns of CDH13 in human head and neck squamous carcinoma cells.

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