急性高眼压大鼠外侧膝状体神经元细胞自噬与凋亡的关系

doi:10.6040/j.issn.1673-3770.0.2017.550

摘要/Abstract

摘要： 目的研究急性高眼压大鼠外侧膝状体神经元细胞自噬与凋亡的关系。方法健康SD大鼠90只,分为假手术组、实验组及3-MA干预组,每组30只,每组又分5个亚时间组,每亚时间组6只。假手术组仅给予前房穿刺,实验组及3-MA组采用前房持续灌注法制作急性青光眼大鼠模型,3-MA组给予3-MA 10 μL玻璃体注射。每组分别于6 h、12 h、24 h、3 d、5 d处死动物,外侧膝状体石蜡切片行免疫组化法检测LC3-Ⅱ及Beclin-1表达量的变化,TUNEL检测神经元凋亡水平,比较自噬与凋亡因子的表达量变化。结果实验组LC3-Ⅱ及Beclin-1阳性细胞表达在6 h出现,24 h表达达高峰,之后逐渐回归至较低水平。3-MA组得到了相似曲线,但各组表达量均低于实验组,组间比较差异有统计学意义(P<0.05)。TUNEL检测凋亡细胞表达在6 h均呈低表达,12 h少量表达,3 d时表达量达峰值,5 d仍可见较高表达,实验组在24 h之后各时间表达量低于3-MA组,差异有统计学意义(P<0.05)。结论大鼠急性高眼压可以导致外侧膝状体神经元自噬与凋亡表达的发生,自噬可能对神经元细胞起到保护作用。

关键词: 外侧膝状体神经元, 凋亡, 急性高眼压, 自噬

Abstract: Objective To investigate the relationship between acute intraocular hypertension and lateral geniculate neurons autophagy and apoptosis in rats. Methods Ninety Sprague-Dawley rats were randomly divided into control, experimental, and 3-methyladenine(3-MA)groups. Each group was divided into 5 subgroups. The control group only received anterior chamber penetration. An acute intraocular hypertension rat model was established using the anterior chamber perfusion technique. The rats in the 3-MA group were injected with 3-MA. At 6 h, 12 h, 24 h, 3 d, and 5 d after the injection, LC3-Ⅱ and Beclin-1 expression in the lateral geniculate nucleus were detected using immunohistochemistry. The apoptotic level in the nerve cells was measured using the dUTP-digoxigenin nick end labeling(TUNEL)assay. Results LC3-Ⅱ and Beclin-1 was expressed in the experimental and 3-MA groups at 6 h, and the levels peaked at 24 h and then gradually returned to baseline levels. However, the expression levels of LC3-Ⅱ and Beclin-1 in the 3-MA group were lower than those in the experimental group at each time point(P<0.05). On the basis of the TUNEL assay results, we found that the apoptosis rate peaked at 3 d in the experimental and 3-MA groups. The apoptosis rate remained high at 5 d. The apoptosis rate was lower in the experimental group than in the 3-MA group at each time point after 24 h(P<0.05). Conclusion Acute intraocular hypertension in rats can lead to lateral geniculate neuron autophagy and apoptosis. Autophagy in lateral geniculate neurons may be a protective factor for neurons.

Key words: Lateral geniculate neurons, Acute intraocular hypertension, Autophagy, Apoptosis

中图分类号:

R774

苏杰,艾昕,马春梅,杨馥宇,黄帅. 急性高眼压大鼠外侧膝状体神经元细胞自噬与凋亡的关系[J]. 山东大学耳鼻喉眼学报, 2018, 32(4): 68-71.

SU Jie, AI Xin, MA Chunmei, YANG Fuyu, HUANG Shuai. Relationship between acute intraocular hypertension and lateral geniculate neuron autophagy and apoptosis in rats[J]. J Otolaryngol Ophthalmol Shandong Univ, 2018, 32(4): 68-71.

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