JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY)

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Expression and mutation analysis of tumor suppressor PTEN in nasopharyngeal carcinoma

CHENG Jin-mei1, XIE Zhen-yu1, LIN Qin1, ZHANG Peng-fei2, LIN Ying1, YI Zi-xiang1， TIAN Wei-zhong2   

  1. 1. Department of Otolaryngology, First Affiliated Hospital of Fujian Medical University， Fuzhou 350005， China；
    2. Department of Pathology, School of Basic Medical Sciences, Fujian Medical University， Fuzhou 350004， China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-10-16 Published:2008-10-16
  • Contact: CHENG Jin-mei

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Abstract: To detect the expression and mutation of tumor suppressor PTEN in nasopharyngeal carcinoma（NPC）， and to　investigate the relationship of the PTEN gene to pathogenesis and development of NPC． MethodsExpression levels of PTEN in 52 cases of NPC were determined by an immunohistochemistry method， and polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP), while silver staining and DNA sequencing were used to detect the mutation in exon 5 and 8 of the PTEN gene in NPC. ResultsDifferences of the PTEN expression were significant between NPC and nasopharyngitis (P＜0.05)． Expressions of PTEN were closely related to lymph node metastasis and clinical TNM stage(P＜0.05). None of 52 cases showed mutation in exon5 and 8 of the PTEN gene in NPC． ConclusionPTEN may have some effects on the pathogenesis and development of NPC, and mutation in exon 5 and 8 of PTEN may not play a main role in the genesis and development of NPC.

Key words: Genes, PTEN, Nasopharyngeal neoplasms, Point mutation, Immunohistochemistry

CLC Number: 

  • R739.6
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