JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2016, Vol. 30 ›› Issue (2): 50-55.doi: 10.6040/j.issn.1673-3770.0.2016.064

Previous Articles     Next Articles

Study of autophagy-related genes atg3, ambra1 expressions in chronic rhinosinusitis without or with nasal polyps accompanied with asthma.

ZHANG Jinling, CAI Xiaolan, LI Xuezhong, FENG Xin, QI Junjun, LIU Dayu   

  1. Department of Otorhinolaryngology, Head and Neck Surgery, Qilu Hospital of Shandong University, Key Laboratory of Otolaryngology of Health Ministry, Shandong University, Jinan 250012, Shandong, China
  • Received:2016-02-19 Online:2016-04-16 Published:2016-04-16

PDF (PC)

1193

Abstract: Objective Autophagy was an important mechanism of protection and defense in organisms. The purpose of the study was observed the expressions of autophagy-related genes Atg3 and Ambra1, investigated the relevance between autophagy, chronic rhinosinusitis(CRS)without or with nasal polyps accompanied with asthma and discussed the probable mechanisms. Methods Collection the mucosa membrane tissue of inferior turbinate in patients with nasal septum deviation(n=24)as control group, research 3 groups included mucous in ostiomeatal complex with chronic rhinosinusitis without nasal polyps(CRSsNP)(n=21), chronic rhinosinusitis with nasal polyps(CRSwNP)(n=22)and chronic rhinosinusitis with nasal polyps accompanied with asthma(CRSwNP accompanied with asthma)(n=18). The expressions of Ambra1 and Atg3 in each group were detected by Immunohistochemistry. Relevance of the autophagy-related 山东大学耳鼻喉眼学报30卷2期 -张金陵,等.自噬相关基因Atg3、Ambra1与慢性单纯性鼻窦炎、鼻息肉及鼻息肉伴发哮喘的相关性研究 \=-genes among the groups was analyzed. Results (1)The expressions of autophagy-related genes Atg3 in control group, CRSsNP group, CRSwNP group and CRSwNP accompanied with asthma group were increased gradually(χ2=31.080, P<0.001). Further pairwise comparisons in the control group with CRSsNP group(P=0.002), the control group and CRSwNP group(P<0.001), control group and CRSwNP accompanied with asthma group(P<0.001), CRSsNP group and CRSwNP accompanied with asthma group(P=0.002), CRSwNP and CRSwNP accompanied with asthma group(P=0.024)expression were statistically significant differences. However, there was not any statistically significant difference between CRSsNP group and CRSwNP group(P=0.304).(2)The expressions of autophagy-related genes Ambra1 in control group, CRSsNP group, CRSwNP group and CRSwNP accompanied with asthma group were all gradually increased(χ2=33.000, P<0.001). Further pairwise comparisons in the control group with CRSsNP group(P=0.009), control group and CRSwNP group(P<0.001), control group and CRSwNP accompanied with asthma group(P<0.001), CRSsNP group and CRSwNP accompanied with asthma group(P<0.001), CRSwNP group and CRSwNP accompanied with asthma group(P=0.009)expression were statistically significant difference, There was not any statistically significant difference between CRSsNP group and CRSwNP group(P=0.205).(3)The expressions of Atg3 and Ambra1 were positively correlated in CRSsNP group(r=0.619, P=0.003), CRSwNP group(r=0.392, P=0.022)and CRSwNP accompanied with asthma group(r=0.552, P= 0.033)(P<0.05). However, there was no statistical correlation between Atg3 and Ambra1 in the contrast group(r=0.316, P=0.133)(P>0.05). Conclusion Autophagy is closely related with the CRS pathogenesis and may be involved in the occurrence of CRSwNP accompanied with asthma. Autophagy related genes Atg3 and Ambra1 may play a synergistic role in the development of CRS. Autophagy can be considered as a potential target for the diagnosis and therapeutic treatment in CRS.

Key words: Chronic rhinosinusitis, Ambra1, Autophagy, Atg3, Asthma

CLC Number: 

  • R765.2
[1] 中华耳鼻咽喉头颈外科杂志编委会鼻科组,中华医学会耳鼻咽喉头颈外科学分会鼻科学组.慢性鼻-鼻窦炎诊断和治疗指南(2012年,昆明)[J].中华耳鼻咽喉头颈外科杂志,2013,48(2):92-94.
[2] Levine B, Mizushima N, Virgin H W. Autophagy in immunity and inflammation[J]. Nature, 2011, 469(7330):323-335.
[3] Jones S A, Mills K H G, Harris J. Autophagy and inflammatory diseases[J]. Immunol Cell Biol, 2013, 91(3):250-258.
[4] Boya P, Reggiori F, Codogno P. Emerging regulation and functions of autophagy[J]. Nat Cell Biol, 2013, 15(7):713-720.
[5] Jong-Ok Pyo, Jihoon Nah, Yong-Keun Jung. Molecules and their functions in autophagy[J]. Exp Mol Med Vol, 2012, 44(2):73-80.
[6] Kadija Abounit, Tiziano M Scarabelli, Roy B McCauley. Autophagy in mammalian cells[J]. World J Biol Chem, 2012, 3(1):1-6.
[7] Fimia G M, Stoykova A, Romagnoli A, et al. Ambral regulates autophagy and development of the nervous system[J]. Nature, 2007, 447(7148):1121-1125
[8] Xu Y, Jagannath C, Liu X D, et al. Toll-like receptor 4 is a sensor for autophagy associated with innate immunity[J]. Immunity, 2007, 27(1):135-144.
[9] Glick D, Barth S, Macleod K F. Autophagy: cellular and molecular mechanisms[J]. J Pathol, 2010, 221(1):3-12.
[10] Poon A H, Chouiali F, Tse S M, et al. Genetic and histologic evidence for autophagy in asthma pathogenesis[J]. Allergy Clin lmmunol, 2012, 129(2):569-571.
[11] Jyothula S S, Eissa N T. Autophagy and role in asthma[J]. Curr Opin Pulm Med, 2013, 19(1):30-35.
[1] FENG Sicong, YU Xiaolan, LOU Dan. Evaluation of the effect of Messerklinger middle turbinate plasty on chronic rhinosinusitis with nasal polyps based on the expression of Ki67 and GM-CSF in vesicular tissue [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2026, 40(3): 31-39.
[2] LI Kai, LUO Dan. Runmu ling formula ameliorates ocular surface damage in dry eye rats by activating the LC3-ATG5 autophagy pathway and inhibiting inflammatory factor expression [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2026, 40(3): 92-101.
[3] ZHAI Xuechun, BIAN Xin, CHEN Jingcai, DENG Jiayu, YE Zi, YANG Pingli. Characteristics and influencing factors of anxiety and depression in patients with chronic frontal sinusitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2026, 40(2): 35-43.
[4] LI Yangsong, YUAN Fayang, YANG Yan, ZHANG Tian, YU Guodong. Bibliometric analysis of biologic agents for the treatment of chronic rhinosinusitis based on the Web of Science database [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(6): 54-64.
[5] PAN Linlin, WAN Jiaming, LI Yue, HE Long. Autophagy-related long noncoding RNA is a prognostic indicator for head and neck squamous cell carcinoma [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(6): 97-107.
[6] ZHANG Jiaqi, YUAN Ye, HONG Chen, GU Min, CHENG Lei, LU Meiping. Mendelian randomization study of gut microbiota, chronic sinusitis, and nasal polyps: Causal relationships and metabolite-mediated effects [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(5): 49-60.
[7] ZHANG Guangling, CHEN Xingxue, WU Tianyi, SUN Zhanwei, WANG Weiwei, LI Shichao,WANG Guangke. The expression and function of Tespa1 in chronic rhinosinusitis with nasal polyps [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(2): 35-42.
[8] CAO Zhengyong, LI Xiaobo. Comparison of the safety and efficacy of short-course postoperative topical glucocorticoid adjuvant therapy for eCRSwNP combined with asthma [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(2): 43-50.
[9] WU Min, LI Zhengyang, MENG Jie, YE Huiping. Molecular mechanisms of programmed cell death and its role in nasopharyngeal carcinoma [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(2): 152-157.
[10] WANG Aowei, SHI Wenjie. Study on correlation between traditional Chinese medicine syndrome types and TFH cell related factors in CRSwNP [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(1): 54-60.
[11] ZHANG Jie, NIMA Jizong, XU Xiaodong, ZHOU Jing, LIU Jianmin, LUO Yirui, DU Jintao, BA Luo. Effect of Crocin on Type 2 inflammation in eosinophilic chronic rhinosinusitis with nasal polyps [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(1): 61-67.
[12] LI Ting, ZHAO Chong, WU Xian. The relationship between miR-34a expression in nasal mucosa tissue and postoperative recurrence in patients with chronic sinusitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(5): 20-25.
[13] CHEN Xingxue, ZHANG Guangling, WU Tianyi, WANG Weiwei, SUN Zhanwei, LI Shichao, WANG Guangke. Analysis of anti-IL-4Rα monoclonal antibody and endoscopic sinus surgery in the treatment of eosinophilic chronic rhinosinusitis with nasal polyps [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(4): 43-54.
[14] ZHANG Shihan, LIU Hongbing. Effect of matrix metalloproteinase on tissue remodeling in chronic rhinosinusitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(3): 116-123.
[15] CHONG Weikun, WANG Juan. Efficacy of Omalizumab in children with moderate and severe allergic asthma and chronic sinusitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(1): 21-26.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd