J Otolaryngol Ophthalmol Shandong Univ ›› 2018, Vol. 32 ›› Issue (5): 66-70.doi: 10.6040/j.issn.1673-3770.0.2018.105

Previous Articles     Next Articles

Expression of IL-35 in allergic rhinitis and its effect on the immunoregulation of T cells

LU Hangui, LIN Xinsheng, YAO Danmian, WEI Yongxin, LI Chuangwei   

  1. Department of Otorhinolaryngology Head and Neck Surgery, Shantou Central Hospital, Shantou 515031, Guangdong, China
  • Received:2018-02-28 Online:2018-09-20 Published:2018-09-20

PDF (PC)

392

Abstract: Objective To investigate the expression of IL-35 in allergic rhinitis(AR)and to explore its effect on the immunoregulation of T cells. Methods A rat model of AR was established by using ovalbumin as the allergen. Rats were randomly divided into the control group, model group, dexamethasone group, and IL-35 intervention group. Anaphylaxis score of rats was analyzed and compared. RT-PCR was used to analyze the mRNA expressions of IL-35, IFN-γ, IL-4, and IL-17 in the nasal mucosa. The expression levels of IL-35, IFN-γ, IL-4, and IL-17 in peripheral blood were measured by enzyme linked immunosorbent assay. Flow cytometry was used to detect the percentage of Th1, Th2, and Th17 cells in peripheral blood. Results IL-35 intervention can significantly reduce the symptoms of AR rats. The expression levels of IL-35 and IFN-γ in the AR model group and in peripheral blood were significantly lower than in the control group, whereas the expression levels were higher in the IL-35 intervention group than in the AR group. IL-4 and IL-17 expression levels were significantly higher in the AR model group than in the control group, whereas the expression levels were lower in the IL-35 intervention group than in the AR model group. The percentage of Th1 cells in the AR model group was significantly lower than that in the control group, whereas the percentage of Th1 cells in the IL-35 intervention group was significantly higher than that in the AR model group. The percentage of Th2 and Th17 cells was significantly higher in the AR model group than that in the control group. The percentage of Th2 and Th17 cells in the IL-35 intervention group was significantly lower than that in the AR model group(P<0.05). Conclusion IL-35 expression was downregulated in AR rats. IL-35 intervention can reduce the inflammatory response by upregulation of IFN-γ and downregulation of IL-4 and IL-17. The mechanism may be related to the regulation of Th1, Th2, and Th17 balance.

Key words: IL-35, Helper T cells, Allergic rhinitis

CLC Number: 

  • R765.4
[1] Seo JH, Kim HY, Jung YH, et al. Interactions between innate immunity genes and early-life risk factors in allergic rhinitis[J]. Allergy, Asthma Immunol Res, 2015, 7(3):241-248.
[2] Yokota M, Suzuki M, Nakamura Y, et al. Cytokine modulation by IL-35 in mice with allergic rhinitis[J]. Am J Rhinol Allergy, 2015, 29(4):251-256.
[3] Shi Y, Dai M, Wu G, et al. Levels of interleukin-35 and its relationship with regulatory T-cells in chronic hepatitis B patients[J]. Viral Immunol, 2015, 28(2):93-100.
[4] Vaughan RA, Gannon NP, Mermier CM, et al. Irisin, a unique non-inflammatory myokine in stimulating skeletal muscle metabolism[J]. J Physiol Biochem, 2015, 71(4):679-689.
[5] 安云芳, 赵长青. 变应性鼻炎鼻黏膜P物质受体的研究[J]. 中华耳鼻咽喉科杂志, 1998, 33(3):139-142. AN Yunfang, ZHAO Changqing. Distribution of substance P receptors in rat nasal mucosa of allergic rhinitis[J]. Chin J Otolaryngol, 1998, 33(3):139-142.
[6] 中华医学会耳鼻咽喉科学分会. 变应性鼻炎诊断标准及疗效评定标准[J]. 中华耳鼻咽喉科杂志,1998,33(3):134-135.
[7] Dixon KO, der Kooij SW, Vignali DAA, et al. Human tolerogenic dendritic cells produce IL-35 in the absence of other IL-12 family members[J]. Eur J Immunol, 2015, 45(6):1736-1747.
[8] Ozkan ZS, Simsek M, Ilhan F, et al. Plasma IL-17, IL-35, interferon-γ, SOCS3 and TGF-βlevels in pregnant women with preeclampsia, and their relation with severity of disease[J]. J Mater Fetal Neonatal Med, 2014, 27(15):1513-1517.
[9] Dong J, Wong CK, Cai Z, et al. Amelioration of allergic airway inflammation in mice by regulatory IL-35 through dampening inflammatory dendritic cells[J]. Allergy, 2015, 70(8):921-932.
[10] Shen P, Roch T, Lampropoulou V, et al. IL-35-producing B cells are critical regulators of immunity during autoimmune and infectious diseases[J]. Nature, 2014, 507(7492):366-370.
[11] Kochetkova I, Thornburg T, Callis G, et al. Oral Escherichia coli colonization factor antigen I fimbriae ameliorate arthritis via IL-35, not IL-27[J]. J Immunol, 2014, 192(2):804-816.
[12] Zongyi Y, Funian Z, Hao L, et al. Interleukin-35 mitigates the function of murine transplanted islet cells via regulation of Treg/Th17 ratio[J]. PloS one, 2017, 12(12):e0189617.
[13] 刘仁慧, 王秀娟, 许利平, 等. 培本中药及其拆方调节哮喘大鼠Th1/Th2平衡的实验研究[J]. 北京中医药大学学报, 2014, 37(3):165-170. LIU Renhui, WANG Xiujuan, XU Liping, et al. Peiben Fang and its disassembled formulas for regulating Th1/Th2 balance in rats with asthma[J]. J Beijing Univ Trad Chin Med, 2014, 37(3):165-170.
[14] Gu ZW, Wang YX, Cao ZW. Neutralization of interleukin-9 ameliorates symptoms of allergic rhinitis by reducing Th2, Th9, and Th17 responses and increasing the Treg response in a murine model[J]. Oncotarget, 2017, 8(9):14314-14324.
[15] Wong CK, Leung TF, Chu I MT, et al. Aberrant expression of regulatory cytokine IL-35 and pattern recognition receptor NOD2 in patients with allergic asthma[J]. Inflammation, 2015, 38(1):348-360.
[16] 唐瑜健. IL-35 蛋白对小鼠铜绿假单胞菌肺炎的干预作用[D]. 泸州:四川医科大学, 2015.
[17] Yin X, Ren X, Liu C, et al. The regulatory effect of IL-35 on the balance of Treg/Th17 cells in allergic rhinitis patients[J]. J Clin Otorhinolaryngol Head Neck Surgery, 2016, 30(3):213-216.
[1] LYU Qian, TANG Yuan, GU Zijun, SHI Sailei, LIU Ping, WAN Wenjin. Current status of benefit finding among patients with allergic rhinitis and its influence factors [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2026, 40(1): 21-28.
[2] GU Min, LU Meiping. Advances in the application of nanomedicine delivery systems in allergen immunotherapy for allergic rhinitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2026, 40(1): 106-111.
[3] QIN Nana, LI Yufen, SUN Yuhao, WEI Jian, LI Qin. Effect of interleukin-13 receptor-α2 on nasal mucosal remodeling in rats with allergic rhinitis by TGF-β1/Smad signaling pathway [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(6): 71-77.
[4] DU Kangli, ZHENG Zhenyu, XU Zhanjiang, ZHANG Yu, CHEN Lu, LU Mengyao. Construction and validation of risk prediction model for nasal septal deviation complicated with chronic sinusitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(6): 78-86.
[5] XIONG Qin, ZHANG Yan, WU Rina, LI Feng, TANG Lixing. Application of intranasal corticosteroids in pediatrics [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(6): 160-167.
[6] XU Xuemeng, FAN Lei, YU Wangbo, JIANG Zhiyue, PAN Chen, HUANG Yongqin. Meta-analysis of the efficacy of omalizumab in combination with specific immunotherapy for allergic rhinitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(5): 26-33.
[7] HUANG Huan, HUA Hongli, DENG Yuqin, JIANG Chengyang, WANG Yuwei, YANG Xinghai. Correlation of allergic rhinitis, tonsil adenoid hypertrophy, and sinusitis in children and analysis of its clinical guiding value [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(5): 34-41.
[8] LIU Yijun, GU Yue, GUAN Dayu, YANG Yucheng, SHEN Yang. The long-term clinical efficacy and safety of vidian neurectomy in refractory allergic rhinitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(5): 42-48.
[9] ZHANG Ting, WANG Meilan, GAO Yingqin. Research progress of IL-35 in allergic rhinitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(5): 139-147.
[10] ZHANG Zhuping, PENG Zican, XIAO Zhenlong, LI Cheng, YU Di, WANG Xinglong, CHEN Wei, Guo Bei. The value of a novel nasal secretion eosinophil cationic protein-myeloperoxidase test paper assay in allergic rhinitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(3): 129-134.
[11] LIU Chang, YANG Jingpu, GAO Yu, WANG Wenjia. Analysis of allergen detection results in children with allergic rhinitis in the Changchun area [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(2): 51-58.
[12] ZHOU Heqing, SHEN Qi. Research progress on biomarkers of traditional Chinese medicine in the treatment of allergic rhinitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(2): 168-176.
[13] WANG Manxian, ZHENG Quan, YANG Liang. Research progress in the treatment of allergic rhinitis with bacterial lysates [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(1): 141-145.
[14] LIU Chang, FANG Hongyan, LIU Xiao, FU Dongna, WANG He, WANG Jing, YANG Jingpu. Analysis of sensitization characteristics of Artemisia pollen in autumn allergic rhinitis in the Changchun area of China [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(5): 13-19.
[15] ZHANG Jie, CHEN Min, SHEN Zhengzheng, WU Yuhua, LIU Yuanhu, SUN Hao, TAN Xinhua, NI Shuren, YANG Shuxun, SHI Xuezheng, NI Xin. Study on the correlation between allergic rhinitis and coronavirus disease-2019 infection and symptoms in children [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(4): 36-42.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd