CXCL10及IFN-γ在上颌窦后鼻孔息肉中的表达及意义

doi:10.6040/j.issn.1673-3770.0.2023.233

摘要/Abstract

摘要： 目的探讨炎性细胞、CXC趋化因子配体10(CXC chemokine ligand 10, CXCL10)及干扰素-γ(interferon-γ,IFN-γ)在上颌窦后鼻孔息肉(antrochoanal polyp, ACP)中的表达及意义。方法收集2015年10月至2020年7月于河北北方学院附属第一医院耳鼻咽喉科就诊的58例患者,其中40例ACP患者、18例上颌窦囊肿患者;分别收集手术样本和术前血清;对手术切除标本进行石蜡包埋、HE染色,计数各类炎性细胞的数量与百分比,对ACP患者上皮增生情况进行综合评分。免疫组织化学染色观察CXCL10和IFN-γ在实验组和对照组的表达及差异情况;酶联免疫吸附实验检测两组血清CXCL10的蛋白浓度,并分析蛋白浓度与两组外周血炎细胞间的相关程度。结果 HE染色显示ACP组织上皮重构,呈异质性,并有炎细胞浸润,以中性粒细胞、淋巴细胞以及浆细胞为主。血常规炎细胞分析显示中性粒细胞与淋巴细胞呈正相关。免疫组化显示ACP组CXCL10和IFN-γ阳性表达强于对照组。ELISA测定ACP组 CXCL10浓度为(32.15±4.60)pg/mL,对照组为(12.11±3.54)pg/mL(P<0.05)。结论 CXCL10及IFN-γ在ACP组中的表达含量明显上调,推测与非Ⅱ型炎症(尤其与Th1炎症因子)有关。

关键词: 上颌窦后鼻孔息肉, 上皮重塑, 内型, CXC趋化因子配体10, 干扰素-γ

Abstract: Objective To investigate the expression and significance of inflammatory cells,CXC chemokine ligand 10(CXCL10), and interferon-γ(IFN-γ)in the antrochoanal polyp(ACP). Methods A total of 58 samples were collected from the First Affiliated Hospital of Hebei North University between October 2015 and July 2020, including 40 cases of ACP and 18 case of maxillary sinus cysts. Preoperative serum and surgical samples were collected. Paraffin embedding and HE staining were performed on surgical specimens, and the number and percentage of various inflammatory cells were counted. The epithelial hyperplasia of patients with ACP was comprehensively scored. The expression of CXCL10 and IFN-γ in ACP and control groups was observed by immunohistochemical staining. The enzyme-linked immunosorbent assay detected the protein concentration of serum CXCL10 in two groups, and the correlation between protein concentration and inflammatory cells in peripheral blood of the two groups was analyzed. Results HE staining revealed obvious epithelial remodeling and heterogeneity with inflammatory cell infiltration, primarily neutrophils, lymphocytes, and plasma cells in group ACP. A positive correlation was found between ACP neutrophils and lymphocytes with the analysis by routine blood examination. Immunohistochemistry showed that CXCL10 and IFN-γ in the experimental group were strongly positive compared with those in the control group. The concentration of CXCL10 was(32.15±4.60)pg/mL vs(12.11± 3.54)pg/mL in ACP and control groups as determined by ELISA(P<0.05). Conclusion The expression of CXCL10 and IFN-γ were significantly upregulated in the ACP group, which may be related to non-type Ⅱ inflammation, especially Th1 inflammatory factors.

Key words: Antrochoanal polyp, Epithelial remodeling, Endotype, CXC chemokine ligand 10, Interferon-γ

中图分类号:

R765.4

张永财,张江,卢慧,邢可欣,吴靖芳,薛刚,李燕萍,刘延彬. CXCL10及IFN-γ在上颌窦后鼻孔息肉中的表达及意义[J]. 山东大学耳鼻喉眼学报, 2024, 38(2): 7-12.

ZHANG Yongcai, ZHANG Jiang, LU Hui, XING Kexin, WU Jingfang, XUE Gang, LI Yanping, LIU Yanbin. Expression and significance of CXCL10 and IFN-γ in antrochoanal polyp[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(2): 7-12.

参考文献 19

[1]	Garaycochea O, van Strahlen CR, Alobid I, et al. Pheno-endotyping antrochoanal nasal polyposis[J]. Curr Allergy Asthma Rep, 2023, 23(3): 165-180. doi:10.1007/s11882-023-01066-1
[2]	Chen CL, Wang YT, Yao Y, et al. Inflammatory endotypes and tissue remodeling features in antrochoanal polyps[J]. Allergy Asthma Immunol Res, 2021, 13(6): 863-881. doi:10.4168/aair.2021.13.6.863
[3]	Jin P, Zi X, Charn TC, et al. Histopathological features of antrochoanal polyps in Chinese patients[J]. Rhinology, 2018, 56(4): 378-385. doi:10.4193/Rhin18.057
[4]	Warman M, Kamar Matias A, Yosepovich A, et al. Inflammatory profile of antrochoanal polyps in the Caucasian population-A histologic study[J]. Am J Rhinol Allergy, 2021, 35(5): 664-673. doi:10.1177/1945892421990529
[5]	Karin N. CXCR3 ligands in cancer and autoimmunity, chemoattraction of effector T cells, and beyond[J]. Front Immunol, 2020, 11: 976. doi:10.3389/fimmu.2020.00976
[6]	闫冰, 刘畅, 王阳, 等. CXCL10在嗜酸性粒细胞型与非嗜酸性粒细胞型慢性鼻-鼻窦炎伴鼻息肉的差异表达及意义[J]. 解剖学报, 2020, 51(5): 688-692. doi:10.16098/j.issn.0529-1356.2020.05.009 YAN Bing, LIU Chang, WANG Yang, et al. Differential expression and significance of CXCL10 in eosinophilic and non-eosinophilic chronic rhinosinusitis with nasal polyps[J]. Acta Anatomica Sinica, 2020, 51(5): 688-692. doi:10.16098/j.issn.0529-1356.2020.05.009
[7]	Sitzia E, Santarsiero S, Marini G, et al. Endotypes of nasal polyps in children: a multidisciplinary approach[J]. J Pers Med, 2023, 13(5): 707. doi:10.3390/jpm13050707
[8]	杨瑞瑞. 白介素-38在慢性鼻-鼻窦炎伴鼻息肉中的表达及临床意义[D]. 太原: 山西医科大学, 2022. doi:10.27288/d.cnki.gsxyu.2022.001247
[9]	于克娜. 上颌窦后鼻孔息肉中纤毛结构及功能异常的研究[D]. 济南: 山东大学, 2022. doi:10.27272/d.cnki.gshdu.2022.000296
[10]	朱玉, 朱新华. TH2细胞因子在2型慢性鼻窦炎伴鼻息肉中的作用机制研究进展[J]. 山东大学耳鼻喉眼学报, 2023, 37(5): 156-161. doi: 10.6040 /1.9sn.1673-3770.0.2022.258 ZHU Yu, ZHU Xinhua. Research progress on the role of TH2 cytokines in Type2 chronic rhinosinusitis with nasal polyps[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2023, 37(5): 156-161. doi: 10.6040 /1.9sn.1673-3770.0.2022.258
[11]	Yuan T, Zheng R, Liu J, et al. Role of yes-associated protein in interleukin-13 induced nasal remodeling of chronic rhinosinusitis with nasal polyps[J]. Allergy, 2021, 76(2): 600-604. doi:10.1111/all.14699
[12]	Carsuzaa F, Béquignon É, Bainaud M, et al. Oncostatin M counteracts the fibrotic effects of TGF-β1 and IL-4 on nasal-polyp-derived fibroblasts: a control of fibrosis in chronic rhinosinusitis with nasal polyps?[J]. Int J Mol Sci, 2022, 23(11): 6308. doi:10.3390/ijms23116308
[13]	熊攀辉, 沈暘, 杨玉成. 基于表型和内在型的慢性鼻窦炎诊治进展[J]. 山东大学耳鼻喉眼学报, 2022, 36(3): 15-19. doi:10.6040/j.issn.1673-3770.0.2022.034 XIONG Panhui, SHEN Yang, YANG Yucheng. Advancements in the diagnosis and treatment of chronic sinusitis based on phenotypes and endotypes[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(3): 15-19. doi:10.6040/j.issn.1673-3770.0.2022.034
[14]	敖天. 慢性鼻窦炎伴鼻息肉的内型研究及其指导下的精准控制与治疗[J]. 山东大学耳鼻喉眼学报, 2022, 36(3): 7-14. doi:10.6040/j.issn.1673-3770.1.2022.005 AO Tian. An endotype study of chronic rhinosinusitis with nasal polyps and precise control and treatment under the guidance[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(3): 7-14. doi:10.6040/j.issn.1673-3770.1.2022.005
[15]	Förster-Ruhrmann U, Szczepek AJ, Pierchalla G, et al. Chemokine expression-based endotype clustering of chronic rhinosinusitis[J]. J Pers Med, 2022, 12(4): 646. doi:10.3390/jpm12040646
[16]	Chen C, Park B, Ragonnaud E, et al. Cancer co-opts differentiation of B-cell precursors into macrophage-like cells[J]. Nat Commun, 2022, 13(1): 5376. doi:10.1038/s41467-022-33117-y
[17]	刘堃, 张杰, 严进, 等. 外周血中性粒细胞数与淋巴细胞数比值、白细胞介素-6预测慢性鼻窦炎鼻内镜术后复发的价值[J]. 蚌埠医学院学报, 2022, 47(4): 514-516. doi:10.13898/j.cnki.issn.1000-2200.2022.04.021 LIU Kun, ZHANG Jie, YAN Jin, et al. Value of neutrophil to lymphocyte ratio and interleukin-6 level in peripheral blood in predicting the recurrence of chronic rhino sinusitis after endoscopic sinus surgery[J]. Journal of Bengbu Medical College, 2022, 47(4): 514-516. doi:10.13898/j.cnki.issn.1000-2200.2022.04.021
[18]	Bayer K, Hamidovic S, Brkic FF, et al. Peripheral eosinophil count and eosinophil-to-lymphocyte ratio are associated with revision sinus surgery[J]. Eur Arch Otorhinolaryngol, 2023, 280(1): 183-190. doi:10.1007/s00405-022-07497-2
[19]	陆汉强, 王慧蓉, 蒋华平, 等. 外周血炎性细胞比值对鼻息肉患者术后复发的判断价值[J]. 齐齐哈尔医学院学报, 2021, 42(9): 744-747. doi:10.3969/j.issn.1002-1256.2021.09.002 LU Hanqiang, WANG Huirong, JIANG Huaping, et al. The value of variation of peripheral blood inflammatory cell ratio in determining postoperative recurrence in patients with nasal polyps[J]. Journal of Qiqihar Medical University, 2021, 42(9): 744-747. doi:10.3969/j.issn.1002-1256.2021.09.002

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