山东大学耳鼻喉眼学报 ›› 2024, Vol. 38 ›› Issue (2): 7-12.doi: 10.6040/j.issn.1673-3770.0.2023.233
张永财1,张江1,卢慧1,邢可欣1,吴靖芳1,薛刚2,李燕萍2,刘延彬2
ZHANG Yongcai1, ZHANG Jiang1, LU Hui1, XING Kexin1, WU Jingfang1, XUE Gang2, LI Yanping2, LIU Yanbin2
摘要: 目的 探讨炎性细胞、CXC趋化因子配体10(CXC chemokine ligand 10, CXCL10)及干扰素-γ(interferon-γ,IFN-γ)在上颌窦后鼻孔息肉(antrochoanal polyp, ACP)中的表达及意义。 方法 收集2015年10月至2020年7月于河北北方学院附属第一医院耳鼻咽喉科就诊的58例患者,其中40例ACP患者、18例上颌窦囊肿患者;分别收集手术样本和术前血清;对手术切除标本进行石蜡包埋、HE染色,计数各类炎性细胞的数量与百分比,对ACP患者上皮增生情况进行综合评分。免疫组织化学染色观察CXCL10和IFN-γ在实验组和对照组的表达及差异情况;酶联免疫吸附实验检测两组血清CXCL10的蛋白浓度,并分析蛋白浓度与两组外周血炎细胞间的相关程度。 结果 HE染色显示ACP组织上皮重构,呈异质性,并有炎细胞浸润,以中性粒细胞、淋巴细胞以及浆细胞为主。血常规炎细胞分析显示中性粒细胞与淋巴细胞呈正相关。免疫组化显示ACP组CXCL10和IFN-γ阳性表达强于对照组。ELISA测定ACP组 CXCL10浓度为(32.15±4.60)pg/mL,对照组为(12.11±3.54)pg/mL(P<0.05)。 结论 CXCL10及IFN-γ在ACP组中的表达含量明显上调,推测与非Ⅱ型炎症(尤其与Th1炎症因子)有关。
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