喉鳞癌组织与相邻正常黏膜的基因表达谱差异

山东大学耳鼻喉眼学报

喉鳞癌组织与相邻正常黏膜的基因表达谱差异

刘蕊¹，时文杰²，刘吉祥²

1. 山东大学生命科学学院生物科学系，山东济南 250100；2. 天津市第一中心医院耳鼻咽喉-头颈外科，天津 300192

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-02-24 发布日期:2007-02-24
通讯作者: 刘蕊

Differentially expressed genes in laryngeal squamous cell carcinoma and normal tissue by cDNA microarray

LIU Rui¹, SHI Wen-jie², LIU Ji-xiang²

1. Department of Biology Science, Shandong University, Jinan 250100, Shandong, China;2. Department of Otolaryngology, Tianjin First Central Hospital, Tianjin 300192

Received:1900-01-01 Revised:1900-01-01 Online:2007-02-24 Published:2007-02-24
Contact: LIU Rui

摘要/Abstract

摘要： 目的：研究喉鳞癌组织与相邻正常黏膜的基因表达谱差异。方法：对2例经病理证实的喉鳞状细胞癌标本和邻近的正常黏膜组织标本与基因表达谱芯片进行杂交，利用激光共聚焦荧光检测系统扫描芯片杂交信号，获得样品中基因序列及表达信息。结果：对比2例喉癌和邻近正常组织的基因表达图谱，发现癌组织和正常组织存在差异表达，2例标本重复出现且差异表达的基因共74个，其中喉癌组织表达上调的基因(R>=5)27个，表达下调的基因(0＜R＜0.2)47个。结论：利用基因表达谱芯片可以在喉鳞状细胞癌组织和邻近的正常组织之间一次比较7000多个基因表达变化，并能找出病理组织中表达异常的基因。

关键词: 喉肿瘤, 鳞状细胞癌, 基因表达, 基因芯片

Abstract: Objective: To screen for the differentially expressed genes in laryngeal squamous carcinoma cells and normal laryngeal tissues using cDNA microarray. Methods: The samples from two laryngeal squamous carcinoma tissues were tested using DNA microarrays. The DNAs were fixed on a glass plate by a series of treatments. The mixed probes were then hybridized to DNA microarrays and scanned for fluorescent signals. Differences between the two tissues were produced. Results: Ran belonging to Ras gene family increased 12.207 times, RAB32 belonging to Ras gene family increased 4.904 times and BIRC5 (Survivin) increased 15.495 times. They were significantly highly expressed in laryngeal cancer. The GFI1B gene decreased 0.0265 times. The GATA1 gene was significantly lowly expressed in one laryngeal cancer and was not expressed in carcinoma cells of another patient. Conclusion: cDNA microarray made in our Lab can recognize more than 7000 gene differences between cancer tissues and normal tissues at the same time and find gene variation. These results accord with other studys.

Key words: Squamous cell carcinoma, Laryngeal neoplasms, Gene expression, DNA microarray

中图分类号:

R739.65

刘蕊,时文杰,刘吉祥 . 喉鳞癌组织与相邻正常黏膜的基因表达谱差异[J]. 山东大学耳鼻喉眼学报, 2007, 21(1): 68-70 .

LIU Rui,SHI Wen-jie,LIU Ji-xiang . Differentially expressed genes in laryngeal squamous cell carcinoma and normal tissue by cDNA microarray[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2007, 21(1): 68-70 .

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