喉癌组织中核表皮生长因子受体与Aurora-A激酶的表达及其临床意义

山东大学耳鼻喉眼学报 ›› 2012, Vol. 26 ›› Issue (5): 16-21.

喉癌组织中核表皮生长因子受体与Aurora-A激酶的表达及其临床意义

许海艳1，彭解人1，区永康1，张琼霞2，张存良1，汤智平1，关中1

1.中山大学孙逸仙纪念医院耳鼻咽喉-头颈外科，广州 510120；2.中山大学肿瘤防治中心，广州 510060

收稿日期:2012-03-19 发布日期:2012-10-16
通讯作者: 关中，硕士生导师。E-mail: dr-guanzhong@yahoo.com.cn
作者简介:许海艳, 硕士。E-mail：xuhy2369@gmail.com.cn
基金资助:
国家自然科学基金面上项目(30873009)

Expression and clinicopathological significance of nuclear epidermal growth factor receptor, Aurora-A kinase in laryngeal squamous cell carcinoma

XU Hai-yan1, PENG Jie-ren1, OU Yong-kang1, ZHANG Qiong-xia2, ZHANG Cun-liang1, TANG Zhi-ping1, GUAN Zhong1

1. Department of Otorhinolaryngology & Head and Neck Surgery, Sun Yatsen Memorial Hospital, Sun Yatsen University, Guangzhou 510120, China; 2. State Key Laboratory of Oncology in South China, Cancer Center, Sun Yatsen University, Guangzhou 510060, China

Received:2012-03-19 Published:2012-10-16

摘要/Abstract

摘要：

目的分析喉癌组织中核表皮生长因子受体(nuclear EGFR)、Aurora-A激酶的表达及其在评价喉癌生物学行为和预后中的价值。方法回顾性分析50例喉癌患者临床病理资料及随访结果，免疫组化法检测肿瘤组织中核表皮生长因子受体(EGFR)、Aurora-A激酶的表达。结果 50例喉癌患者的组织中，核EGFR、Aurora-A激酶阳性表达率分别为50%(25/50)、58%(29/50)，均高于癌旁组织(P<0.05)；核EGFR的表达与喉癌的原发灶分期、区域淋巴结转移、复发有关(P均<0.05)；Aurora-A的表达与原发灶分期、区域淋巴结转移有关(P值均<0.05)；核EGFR与Aurora-A表达的相关分析差异有统计学意义(r=0.446，P<0.05)；KaplanMeier生存分析显示患者3年和5年生存率分别为84.0%和67.8%，Log-rank检验显示原发灶分期、区域淋巴结转移、核EGFR、Aurora-A的表达和患者预后有关(P<0.05)。Cox比例分析模型分析显示原发灶分期、区域淋巴结转移、核EGFR表达为影响喉癌患者预后的独立危险因素(P<0.05)。结论在喉癌发生和发展过程中存在核EGFR、Aurora-A激酶的异常表达，并与肿瘤的生物学行为和预后有关。

关键词: 喉鳞状细胞癌；核表皮生长因子受体；Aurora-A激酶

Abstract:

Objective To investigate expressions of nuclear EGFR and Aurora-A kinase in laryngeal squamous cell carcinoma, and to explore their associations with the clinicopathological feathers. Methods Expressions of nuclear EGFR and Aurora-A kinase were analyzed in 50 laryngeal squamous cell carcinoma samples and 10 normal tissues adjacent to the carcinomas by immunohistochemistry. Results Nuclear EGFR and Aurora-A kinase were up-regulated in most laryngeal squamous cell carcinoma samples but not in normal tissues. The difference of weighted scores between these two groups was significant(P<0.05). Although there were no correlations between expression of nuclear EGFR and some tumor parameters, such as age, gender, tumor site, expression of nuclear EGFR was significantly associated with tumor T staging(P<0.05), lymph node metastasis(P<0.05) and relapse(P<0.05). There were no correlations between expression of Aurora-A kinase and some tumor parameters, such as age, gender, tumor site, relapse (P>0.05), while expressions of Aurora-A kinase was significantly associated with T staging (P<0.05), lymph node metastasis (P<0.05). In laryngeal squamous cell carcinoma tissues, there were positive correlation between expressions of nuclear EGFR and Aurora-A kinase (P<0.05). Kaplan-Meier survival analysis showed the 3, 5year survival rates of high nuclear EGFR and Aurora-A kinase were significantly lower than in the contrary groups(84.0%, P=0.020; 67.8%, P=0.006, respectively). Log-rank test demonstrated that patients with high tumor T staging, lymph node metastasis, and expressions of nuclear EGFR and Aurora-A kinase had a poor outcome (P<0.05). While Cox multiple factors analysis showed that tumor T staging, lymph node metastasis and nuclear EGFR were independent prognostic factors of laryngeal squamous cell cancer(P=0.045, 0.018, 0.088, respectively). Conclusion Nuclear EGFR and Aurora-A kinase are associated with biological behaviors and prognosis of laryngeal squmous cell cancer.

Key words: Laryngeal squamous cell carcinoma; Nuclear EGFR; AuroraA kinase

中图分类号:

R739.65

许海艳1，彭解人1，区永康1，张琼霞2，张存良1，汤智平1，关中1. 喉癌组织中核表皮生长因子受体与Aurora-A激酶的表达及其临床意义[J]. 山东大学耳鼻喉眼学报, 2012, 26(5): 16-21.

XU Hai-yan1, PENG Jie-ren1, OU Yong-kang1, ZHANG Qiong-xia2, ZHANG Cun-liang1, TANG Zhi-ping1, GUAN Zhong1. Expression and clinicopathological significance of nuclear epidermal growth factor receptor, Aurora-A kinase in laryngeal squamous cell carcinoma[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2012, 26(5): 16-21.

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