山东大学耳鼻喉眼学报 ›› 2015, Vol. 29 ›› Issue (2): 74-80.doi: 10.6040/j.issn.1673-3770.0.2014.351
张营春, 杜祥阁, 颜昕, 王翠, 赵博军
ZHANG Ying-chun, DU Xiang-ge, YAN Xin, WANG Cui, ZHAO Bo-jun
摘要: 目的 探讨尼古丁对人视网膜色素上皮(RPE)细胞和血管内皮细胞(HUVECs)中血管内皮生长因子(VEGF)表达及形成新生血管能力的影响。方法 MTT法检测尼古丁对两种细胞增殖的影响;划痕及成管实验检测其对HUVECs迁移、成管的影响;RT-PCR和Western blotting法检测两种细胞经尼古丁处理后VEGF、PEDF mRNA及蛋白的表达。结果 尼古丁促进HUVECs增殖、迁移、成管(P<0.05), 但对ARPE-19细胞增殖无影响(P>0.05), 且高浓度(100 μmol/L)时抑制细胞增殖(P<0.01)。尼古丁以剂量和时间依赖方式促进两种细胞VEGF mRNA及蛋白的表达, 抑制PEDF mRNA及蛋白的表达, 上调VEGF/PEDF基因表达的比率(P<0.05)。结论 尼古丁可上调ARPE-19细胞和HUVECs中VEGF/PEDF基因表达的比率, 促进HUVECs增殖、迁移、成管。
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