山东大学耳鼻喉眼学报 ›› 2015, Vol. 29 ›› Issue (3): 16-19.doi: 10.6040/j.issn.1673-3770.0.2015.087

• 论著 • 上一篇    下一篇

雪旺细胞及神经干细胞与Chitosan-PLGA导管的细胞相容性

门永芝, 程宇晟, 李煜, 董频, 於子卫   

  1. 上海交通大学附属上海市第一人民医院耳鼻咽喉-头颈外科, 上海 200080
  • 收稿日期:2015-02-10 修回日期:2015-04-10 发布日期:2015-06-16
  • 通讯作者: 於子卫。E-mail:david545yu@163.com E-mail:david545yu@163.com
  • 作者简介:门永芝。E-mail:menlady2012@163.com
  • 基金资助:
    上海交通大学王宽诚医学奖励基金及国家自然基金面上项目(81170925)

Cytocompatibility research of schwann cells and neural stem cells co-seeded on electron spinning chitosan-PLGA

MEN Yongzhi, CHENG Yusheng, LI Yu, DONG Pin, YU Ziwei   

  1. Department of Otolaryngology & Head and Neck Surgery, Shanghai General Hospital, Shanghai Jiao Tong University Affiliated First People's Hospital, Shanghai 200080, China
  • Received:2015-02-10 Revised:2015-04-10 Published:2015-06-16

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摘要： 目的 研究雪旺细胞(SCs)、神经干细胞(NSCs)与内置纵行排列纳米纤维丝的静电纺丝壳聚糖-聚羟基乙酸 (Chitosan-PLGA)纳米导管材料的细胞相容性, 探讨细胞在导管材料上的黏附及定向生长情况。方法 实验分为SCs组、NSCs组、SCs-NSCs共培养组, 3组细胞分别与Chitosan-PLGA纳米导管材料复合培养, 四甲基偶氮唑蓝(MTT)比色法观察各组细胞存活率, 扫描电镜下观察SCs、NSCs在Chitosan-PLGA上的生长。结果 细胞存活率排序由高至低依次为共培养细胞组、SCs组、NSCs组。SCs及NSCs能贴附在Chitosan-PLGA 内置纳米纤维丝上生长并沿纤维丝定向生长。结论 雪旺细胞及神经干细胞共培养可促进细胞与Chitosan-PLGA材料的相容性。内置纵行排列的纳米纤维丝有引导细胞定向生长与迁移的作用。

关键词: 静电纺丝, Chitosan-PLGA, 神经干细胞, 雪旺细胞, 定向生长

Abstract: Objective To investigate cytocompatibility of the electron spinning chitosan-PLGA nerve conduits with Schwann cells (SCs)and neural stem cells(NSCs) as well as cells adhesion and migration on the arranged nanofibers inside the lumen. Methods Experiment was divided into three groups: the SCs group, the NSCs group and the SCs-NSCs co-culture group. Cells in these groups were co-seeded with Chitosan-PLGA. The biocompatibility and absorbability of SCs and NSCs were detected by MTT assays. Scanning electron microscopy(SEM) was used to detect cells adhesion and migration on the axially arranged nanofibers inside the lumen. Results Cell survival rate of the co-culture group was the highest, the higher in the SCs group, and the lowest in the NSCs group. SCs and NSCs had good compatibility and adhesion with Chitosan-PLGA, and the axial nanofibers in the lumen guided the growth of neurites in a particular direction. Conclusion Co-culture of SCs and NSCs can facilitate cells compatibility and adhesion with 3D Chitosan-PLGA, and the axial arranged nanofibers within the lumen has the effect of directional guide cell growth and migration.

Key words: Electron spinning, Chitosan-PLGA, Schwann cells, Neural stem cells, Directional growth

中图分类号: 

  • R745.1
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