p-ERK2及ERK2在鼻息肉组织的表达及其意义

doi:10.6040/j.issn.1673-3770.0.2017.522

摘要/Abstract

摘要： 目的检测磷酸化细胞外信号调节激酶2(p-ERK2)及细胞外信号调节激酶2(ERK2)在鼻息肉(NP)组织的定位及表达情况;初步探讨p-ERK2/ERK2在NP发病中的临床意义。方法应用间接免疫荧光技术(IIF)及蛋白免疫印记技术(WB)检测p-ERK2/ERK2在NP及正常鼻黏膜组织的定位及表达水平,并探讨其临床意义。结果 NP:p-ERK2主要表达在增生的上皮细胞、炎症细胞以及腺上皮细胞,主要位于胞核;ERK2主要表达在腺上皮细胞、炎症细胞,主要位于胞质。正常鼻黏膜组织:p-ERK2主要表达在炎症细胞、腺上皮细胞,主要位于胞核;ERK2主要位于炎症细胞,主要位于胞质。荧光强度值:p-ERK2在NP和正常鼻黏膜组织的表达有统计学差异(P<0.05);ERK2在NP和正常鼻黏膜组织的表达无统计学差异(P>0.05)。WB灰度值:p-ERK2在NP和正常鼻黏膜组织的表达有统计学差异(P<0.05);ERK2在NP和正常鼻黏膜组织的表达无统计学差异(P>0.05)。结论 p-ERK2在NP的发病机制中可能具有重要作用,而ERK2与NP的发生可能无关。ERK2在NP组及正常鼻黏膜组织均表达,提示ERK2可能参与鼻黏膜正常生理功能的维持。

关键词: 细胞外信号调节激酶2, ERK信号通路, 磷酸化细胞外信号调节激酶2, 鼻窦炎, 鼻息肉

Abstract: Objective To detect the expression of p-ERK2/ ERK2 in nasal polyps(NPs)and to explore the clinical significance of p-ERK2/ ERK2 in the pathogenesis of NPs. Methods Indirect immunofluorescence and western blotting were used to detect the expression of p-ERK2/ ERK2 in NPs and normal nasal mucosa. Results In the NP group, p-ERK2 was primarily expressed in the proliferative epithelial cells, inflammatory cells, and glandular epithelial cells, and was localized predominantly in the nucleus; ERK2 was mainly expressed in the glandular epithelial cells and inflammatory cells, and was localized predominantly in the cytoplasm. In the normal nasal mucosa group, p-ERK2 was primarily expressed in the inflammatory cells and glandular epithelial cells, and was localized predominantly in the nucleus; ERK2 was mainly expressed in the inflammatory cells, and was localized predominantly in the cytoplasm. Considering fluorescence intensity as an index for expression, p-ERK2 expression showed a significant difference between the NP and normal nasal mucosa groups(P<0.05), while ERK2 expression did not(P>0.05). Using the gray values from Western blotting, p-ERK2 expression showed a significant difference between the two groups(P<0.05), while ERK2 expression did not(P>0.05). Conclusion p-ERK2 might play an important role in the pathogenesis of nasal polyps. However, ERK2 might not be involved in the initiation of nasal polyps; ERK2 was not only expressed in nasal polyps, but also in the normal nasal mucosa, suggesting that ERK2 might be involved in the maintenance of the normal physiological function of the nasal mucosa.

Key words: Sinusitis, ERK signal pathway, p-ERK2, ERK2, Nasal polyps

中图分类号:

R765.2

李宏梅,王兰,高照慧,王莹,薛金梅. p-ERK2及ERK2在鼻息肉组织的表达及其意义[J]. 山东大学耳鼻喉眼学报, 2018, 32(3): 42-46.

LI Hongmei, WANG Lan, GAO Zhaohui, WANG Ying, XUE Jinmei. The expression and significance of p-ERK2/ ERK2 in nasal polyps[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2018, 32(3): 42-46.

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