山东大学耳鼻喉眼学报 ›› 2020, Vol. 34 ›› Issue (6): 72-76.doi: 10.6040/j.issn.1673-3770.0.2019.618

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CPS1在喉鳞癌组织中的表达及临床意义

范义燕1,2,张肖林1,刘秀珍3,尹晶晶1,袁进1,王延飞1,陈军1   

  1. 1. 滨州医学院附属医院 耳鼻咽喉头颈外科, 山东 滨州 256603;
    2. 胜利油田中心医院 耳鼻喉科, 山东 东营 257000;
    3. 滨州医学院附属医院 临床医学实验室, 山东 滨州 256603
  • 发布日期:2021-01-11
  • 通讯作者: 陈军. E-mail:bzchenjun@126.com

Expression and clinical significance of CPS1 in laryngeal squamous cell carcinoma

FAN Yiyan1,2, ZHANG Xiaolin1, LIU Xiuzhen3, YIN Jingjing1, YUAN Jin1, WANG Yanfei1, CHEN Jun1   

  1. 1. Otorhinolaryngology & Head and Neck Surgery, Binzhou Medical University Hospital, Binzhou 256603, Shandong, China;
    2. Department of Otolaryngology, Shengli Oilfield Central Hospital, Dongying 257000, Shandong, China;
    3. Clinical Medical Laboratory, Binzhou Medical University Hospital, Binzhou 256603, Shandong, China
  • Published:2021-01-11

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摘要： 目的 研究氨基甲酰磷酸合成酶1(CPS1)在喉鳞癌患者组织中的表达水平及与临床病理指标的相关性,探讨CPS1对喉鳞癌的临床意义。 方法 采用逆转录实时定量聚合酶链反应(RT-qPCR)及Western blot免疫印迹技术,分别检测喉鳞癌组织及癌旁组织中CPS1 mRNA及蛋白表达水平,同时采用免疫组织化学法检测 CPS1在喉鳞癌组织中的表达水平及其结构中的分布情况。 结果 RT-qPCR 和Western blot结果显示,与癌旁组织相比,喉鳞癌组织CPS1 mRNA 和蛋白表达明显增高,表达差异有统计学意义(P<0.01);免疫组化结果显示CPS1表达与肿瘤分化程度、临床分期、淋巴结转移等因素密切相关(P<0.05),而与患者年龄、性别、吸烟史、饮酒史及肿瘤部位无关(P>0.05)。 结论 在喉鳞癌组织中CPS1的mRNA和蛋白表达明显升高,且与临床进展密切相关,说明CPS1在喉癌发生及转移过程中起到一定的作用,可能作为喉癌诊断的重要指标。

关键词: 氨基甲酰磷酸合成酶1, 喉鳞状细胞癌, 临床进展

Abstract: Objective To study the expression levels of carbamoyl phosphate synthase I(CPS1)in laryngeal squamous cell carcinoma and adjacent non-cancerous tissues and reveal its possible correlation with clinical parameters, and explore the clinical significance of CPS1 in the disease. Methods The real-time quantitative polymerase chain reaction(RT-qPCR)was used to measure the expression of CPS1 mRNA in laryngeal squamous cell carcinoma and adjacent tissues. Western blotting and immunohistochemistry were used to detect expression levels of the CPS1 protein and its distribution in laryngeal squamous cell carcinoma tissues. Results The results of RT-qPCR and western blot analysis showed that the mRNA and protein expression levels of CPS1 in laryngeal squamous cell carcinoma were significantly higher than that in adjacent tissues(P<0.01). Immunohistochemistry showed the same trend in CPS1 protein levels. The expression of CPS1 was closely related to the degree of tumor differentiation, clinical stage, and lymph node metastasis(P<0.05), but not to the patients' age, gender, smoking history, drinking history, and tumor site(P>0.05). Conclusion The expression of CPS1 in laryngeal squamous cell carcinoma is significantly higher than in the adjacent tissues, and is closely related to clinical progression. These results suggest that CPS1 may play a role in the occurrence and metastasis of laryngeal cancer, and is a diagnostic indicator for laryngeal cancer.

Key words: Carbamyl phosphate synthase 1, Laryngeal squamous cell carcinoma, Clinical progress

中图分类号: 

  • R739.65
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