半乳糖凝集素10与慢性鼻窦炎伴鼻息肉

doi:10.6040/j.issn.1673-3770.0.2020.163

摘要/Abstract

摘要： 半乳糖凝集素10(Galectin-10)被发现已有很长的历史,但是探索其病理作用的研究很少。近几年,半乳糖凝集素10受到越来越多的关注,特别是其在气道嗜酸性粒细胞疾病中研究越来越多。综述了Galectin-10历史回顾及其在慢性鼻窦炎中的相关研究,Galectin-10/CLC蛋白不仅仅可作为以2型免疫反应为特征的CRSwNP的生物标志物,而且可能在其发病机制中发挥一定作用,并且很有希望作为以2型免疫反应为特征的CRSwNP的药物靶点。

关键词: Galectin-10, CLC, 嗜酸性粒细胞, CRSwNP, EETosis

Abstract: Galectin-10(CLC, Charcot-Leyden crystal)was discovered a long ago, but until recently, few studies have explored its role in pathophysiology. In recent years, galectin-10 has received considerable attention, especially for its role in airway eosinophil diseases. Here, we provide a brief historical review of galectin-10 and related research focused on chronic rhinosinusitis. Galectin-10 is a biomarker for chronic rhinosinusitis with nasal polyps characterized by a type 2 inflammatory response and may play an important role in its pathogenesis. Thus, it is a promising potential drug target for this type of CRSwNP.

Key words: Galectin-10, CLC, Eosinophils, CRSwNP, EETosis

中图分类号:

R765.4

李春花,刘肖,刘红兵. 半乳糖凝集素10与慢性鼻窦炎伴鼻息肉[J]. 山东大学耳鼻喉眼学报, 2021, 35(3): 106-111.

LI Chunhua, LIU Xiao,LIU Hongbing. Galectin-10 and chronic rhinosinusitis with nasal polyps[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2021, 35(3): 106-111.

参考文献

[1] Fokkens W J, Lund V J, Hopkins C, et al. European Position Paper on Rhinosinusitis and Nasal Polyps 2020[J]. Rhinology, 2020, 58(Suppl S29):1-464. doi:10.4193/Rhin20.600.
[2] Su JY. A brief history of Charcot-Leyden crystal protein/galectin-10 research[J]. Molecules, 2018, 23(11): E2931. doi:10.3390/molecules23112931.
[3] Johannes L, Jacob R, Leffler H. Galectins at a glance[J]. J Cell Sci, 2018, 131(9): jcs208884. doi:10.1242/jcs.208884.
[4] Rao SP, Ge XN, Sriramarao P. Regulation of eosinophil recruitment and activation by galectins in allergic asthma[J]. Front Med(Lausanne), 2017, 4: 68. doi:10.3389/fmed.2017.00068.
[5] Grozdanovic MM, Doyle CB, Liu L, et al. Charcot-Leyden crystal protein/galectin-10 interacts with cationic ribonucleases and is required for eosinophil granulogenesis[J]. J Allergy Clin Immunol, 2020,146(2):377-389. doi:10.1016/j.jaci.2020.01.013.
[6] Yang RY, Rabinovich GA, Liu FT. Galectins: structure, function and therapeutic potential[J]. Expert Rev Mol Med, 2008, 10: e17. doi:10.1017/s1462399408000719.
[7] Boscher C, Dennis JW, Nabi IR. Glycosylation, galectins and cellular signaling[J]. Curr Opin Cell Biol, 2011, 23(4): 383-392. doi:10.1016/j.ceb.2011.05.001.
[8] Swaminathan GJ, Leonidas DD, Savage MP, et al. Selective recognition of mannose by the human eosinophil Charcot-Leyden crystal protein(galectin-10): a crystallographic study at 1.8 A resolution[J]. Biochemistry, 1999, 38(46): 15406. doi:10.1021/bi995093f.
[9] Su JY, Gao J, Si YL, et al. Galectin-10: a new structural type of prototype galectin dimer and effects on saccharide ligand binding[J]. Glycobiology, 2018, 28(3): 159-168. doi:10.1093/glycob/cwx107.
[10] Charcot JM, Robin C. Observation de leucocythemie[J]. Mem. Soc. Biol, 1853(5):44-50.
[11] Leyden E. Zur kenntniss Des bronchial-asthma[J]. Archiv F Pathol Anat, 1872, 54(4): 324-344. doi:10.1007/bf01997025.
[12] Thompson JH, Paddock FK. The significance of Charcot-Leyden crystals[J]. N Engl J Med, 1940, 223(23): 936-939. doi:10.1056/nejm194012052232304.
[13] Neumann A. über Die natur der Charcot-Leyden-böttcher-Neumann-krystalle.(bemerkungen zur gleichnamigen arbeit von wrede-boldt-buch.)[J]. Hoppe-Seyler's Zeitschrift Für Physiol Chemie, 1928, 173(1/2): 69-71. doi:10.1515/bchm2.1928.173.1-2.69.
[14] Ayres WW, Starkey NM. Studies on Charcot-Leyden crystals[J]. Blood, 1950, 5(3): 254-266. doi:10.1182/blood.v5.3.254.254.
[15] Guo L, Johnson RS, Schuh JC. Biochemical characterization of endogenously formed eosinophilic crystals in the lungs of mice[J]. J Biol Chem, 2000, 275(11): 8032-8037. doi:10.1074/jbc.275.11.8032.
[16] Weller PF, Goetzl EJ, Austen KF. Identification of human eosinophil lysophospholipase as the constituent of Charcot-Leyden crystals[J]. Proc Natl Acad Sci USA, 1980, 77(12): 7440-7443. doi:10.1073/pnas.77.12.7440.
[17] Huffnagle GB, Boyd MB, Street NE, et al. IL-5 is required for eosinophil recruitment, crystal deposition, and mononuclear cell recruitment during a pulmonary Cryptococcus neoformans infection in genetically susceptible mice(C57BL/6)[J]. J Immunol, 1998, 160(5): 2393-2400.
[18] Hoenerhoff MJ, Starost MF, Ward JM. Eosinophilic crystalline pneumonia as a major cause of death in 129S4/SvJae mice[J]. Vet Pathol, 2006, 43(5): 682-688. doi:10.1354/vp.43-5-682.
[19] Wilkerson EM, Johansson MW, Hebert AS, et al. The peripheral blood eosinophil proteome[J]. J Proteome Res, 2016, 15(5): 1524-1533. doi:10.1021/acs.jproteome.6b00006.
[20] Ackerman SJ, Corrette SE, Rosenberg HF, et al. Molecular cloning and characterization of human eosinophil Charcot-Leyden crystal protein(lysophospholipase). Similarities to IgE binding proteins and the S-type animal lectin superfamily[J]. J Immunol, 1993, 150(2): 456-468.
[21] Ackerman SJ, Weil GJ, Gleich GJ. Formation of Charcot-Leyden crystals by human basophils[J]. J Exp Med, 1982, 155(6): 1597-1609. doi:10.1084/jem.155.6.1597.
[22] Dvorak AM, Letourneau L, Weller PF, et al. Ultrastructural localization of Charcot-Leyden crystal protein(lysophospholipase)to intracytoplasmic crystals in tumor cells of primary solid and papillary epithelial neoplasm of the pancreas[J]. Lab Invest, 1990, 62(5): 608-615.
[23] Kubach J, Lutter P, Bopp T, et al. Human CD4⁺CD25⁺ regulatory T cells: proteome analysis identifies galectin-10 as a novel marker essential for their anergy and suppressive function[J]. Blood, 2007, 110(5): 1550-1558. doi:10.1182/blood-2007-01-069229.
[24] Noh S, Jin S, Park CO, et al. Elevated galectin-10 expression of IL-22-producing T cells in patients with atopic dermatitis[J]. J Invest Dermatol, 2016, 136(1): 328-331. doi:10.1038/JID.2015.369.
[25] Ali ND, Weissmann D. Charcot-Leyden crystals in T-cell lymphoblastic lymphoma[J]. Blood, 2017, 129(3): 394. doi:10.1182/blood-2016-10-743179.
[26] Lingblom C, Andersson J, Andersson K, et al. Regulatory eosinophils suppress T cells partly through galectin-10[J]. J Immunol, 2017, 198(12): 4672-4681. doi:10.4049/jimmunol.1601005.
[27] Zhou Z, Tenen DG, Dvorak AM, et al. The gene for human eosinophil Charcot-Leyden crystal protein directs expression of lysophospholipase activity and spontaneous crystallization in transiently transfected COS cells[J]. J Leukoc Biol, 1992, 52(6): 588-595. doi:10.1002/jlb.52.6.588.
[28] Dvorak AM, Letourneau L, Login GR, et al. Ultrastructural localization of the Charcot-Leyden crystal protein(lysophospholipase)to a distinct crystalloid-free granule population in mature human eosinophils[J]. Blood, 1988, 72(1): 150-158.
[29] Calafat J, Janssen H, Knol EF, et al. Ultrastructural localization of Charcot-Leyden crystal protein in human eosinophils and basophils[J]. Eur J Haematol, 1997, 58(1): 56-66. doi:10.1111/j.1600-0609.1997.tb01411.x.
[30] Su JY, Gao J, Si YL, et al. Galectin-10: a new structural type of prototype galectin dimer and effects on saccharide ligand binding[J]. Glycobiology, 2018, 28(3): 159-168. doi:10.1093/glycob/cwx107.
[31] Ueki S, Miyabe Y, Yamamoto Y, et al. Charcot-Leyden crystals in eosinophilic inflammation: active cytolysis leads to crystal formation[J]. Curr Allergy Asthma Rep, 2019, 19(8): 35. doi:10.1007/s11882-019-0868-0.
[32] Grozdanovic MM, Doyle CB, Liu L, et al. Charcot-Leyden crystal protein/galectin-10 interacts with cationic ribonucleases and is required for eosinophil granulogenesis[J]. J Allergy Clin Immunol, 2020: S0091-S6749(20)30100-7. doi:10.1016/j.jaci.2020.01.013.
[33] Ueki S, Tokunaga T, Melo RCN, et al. Charcot-Leyden crystal formation is closely associated with eosinophil extracellular trap cell death[J]. Blood, 2018, 132(20): 2183-2187. doi:10.1182/blood-2018-04-842260.
[34] Su JY, Song CY, Si YL, et al. Identification of key amino acid residues determining ligand binding specificity, homodimerization and cellular distribution of human galectin-10[J]. Glycobiology, 2019, 29(1): 85-93. doi:10.1093/glycob/cwy087.
[35] Dyer KD, Rosenberg HF. Eosinophil Charcot-Leyden crystal protein binds to beta-galactoside sugars[J]. Life Sci, 1996, 58(23): 2073-2082. doi:10.1016/0024-3205(96)00201-9.
[36] Negrete-Garcia MC, Jiménez-Torres CY, Alvarado-Vásquez N, et al. Galectin-10 is released in the nasal lavage fluid of patients with aspirin-sensitive respiratory disease[J]. Sci World J, 2012: 474020. doi:10.1100/2012/474020.
[37] Chua JC, Douglass JA, Gillman A, et al. Galectin-10, a potential biomarker of eosinophilic airway inflammation[J]. PLoS One, 2012, 7(8): e42549. doi:10.1371/journal.pone.0042549.
[38] Nyenhuis SM, Alumkal P, Du J, et al. Charcot-Leyden crystal protein/galectin-10 is a surrogate biomarker of eosinophilic airway inflammation in asthma[J]. Biomark Med, 2019, 13(9): 715-724. doi:10.2217/bmm-2018-0280.
[39] Persson EK, Verstraete K, Heyndrickx I, et al. Protein crystallization promotes type 2 immunity and is reversible by antibody treatment[J]. Science, 2019, 364(6442): eaaw4295. doi:10.1126/science.aaw4295.
[40] Liu C, Yan B, Qi SH, et al. Predictive significance of Charcot-Leyden crystals for eosinophilic chronic rhinosinusitis with nasal polyps[J]. Am J Rhinol Allergy, 2019, 33(6): 671-680. doi:10.1177/1945892419860646.
[41] Wu D, Yan B, Wang Y, et al. Charcot-Leyden crystal concentration in nasal secretions predicts clinical response to glucocorticoids in patients with chronic rhinosinusitis with nasal polyps[J]. J Allergy Clin Immunol, 2019, 144(1): 345-348.e8. doi:10.1016/j.jaci.2019.03.029.
[42] Qi S, Yan B, Liu C, et al. Predictive significance of Charcot-Leyden Crystal mRNA levels in nasal brushing for nasal polyp recurrence[J]. Rhinology, 2020, 58(2): 166-174. doi:10.4193/Rhin19.296.
[43] Ueki S, Konno Y, Takeda M, et al. Eosinophil extracellular trap cell death-derived DNA traps: Their presence in secretions and functional attributes[J]. J Allergy Clin Immunol, 2016, 137(1): 258-267. doi:10.1016/j.jaci.2015.04.041.
[44] Ueki S, Tokunaga T, Fujieda S, et al. Eosinophil ETosis and DNA traps: a new look at eosinophilic inflammation[J]. Curr Allergy Asthma Rep, 2016, 16(8): 54. doi:10.1007/s11882-016-0634-5.
[45] Gevaert E, Zhang N, Krysko O, et al. Extracellular eosinophilic traps in association with Staphylococcus aureus at the site of epithelial barrier defects in patients with severe airway inflammation[J]. J Allergy Clin Immunol, 2017, 139(6): 1849-1860.e6. doi:10.1016/j.jaci.2017.01.019.
[46] Ueki S, Miyabe Y, Yamamoto Y, et al. Charcot-Leyden crystals in eosinophilic inflammation: active cytolysis leads to crystal formation[J]. Curr Allergy Asthma Rep, 2019, 19(8): 35. doi:10.1007/s11882-019-0868-0.
[47] Franklin BS, Mangan MS, Latz E. Crystal Formation in Inflammation[J]. Annu Rev Immunol,2016,34:173-202. doi: 10.1146/annurev-immunol-041015-055539.
[48] Mulay SR, Desai J, Kumar SV, et al. Cytotoxicity of crystals involves RIPK3-MLKL-mediated necroptosis[J]. Nat Commun, 2016, 7: 10274. doi:10.1038/ncomms10274.
[49] Klion AD. Charcot-Leyden crystals: solving an Enigma[J]. Blood, 2018, 132(20): 2111-2112. doi:10.1182/blood-2018-09-873653.
[50] Bachert C, Humbert M, Hanania NA, et al. Staphylococcus aureus and its IgE-inducing enterotoxins in asthma: current knowledge[J]. Eur Respir J, 2020, 55(4): 1901592. doi:10.1183/13993003.01592-2019.
[51] Lin H, Li ZP, Lin D, et al. Role of NLRP3 inflammasome in eosinophilic and non-eosinophilic chronic rhinosinusitis with nasal polyps[J]. Inflammation, 2016, 39(6): 2045-2052. doi:10.1007/s10753-016-0442-z.
[52] Rodríguez-Alcázar JF, Ataide MA, Engels G, et al. Charcot-Leyden crystals activate the NLRP3 inflammasome and cause IL-1β inflammation in human macrophages[J]. J Immunol, 2019, 202(2): 550-558. doi:10.4049/jimmunol.1800107.
[53] Kim DK, Kim JY, Han YE, et al. Elastase-positive neutrophils are associated with refractoriness of chronic rhinosinusitis with nasal polyps in an Asian population[J]. Allergy Asthma Immunol Res, 2020, 12(1): 42-55. doi:10.4168/aair.2020.12.1.42.
[54] Lan F, Zhang L. Understanding the role of neutrophils in refractoriness of chronic rhinosinusitis with nasal polyps[J]. Allergy Asthma Immunol Res, 2020, 12(1): 1-3. doi:10.4168/aair.2020.12.1.1.
[55] Gevaert E, Delemarre T, De Volder J, et al. Charcot-Leyden crystals promote neutrophilic inflammation in patients with nasal polyposis[J]. J Allergy Clin Immunol, 2020, 145(1): 427-430.e4. doi:10.1016/j.jaci.2019.08.027.
[56] Udell IJ, Gleich GJ, Allansmith MR, et al. Eosinophil granule major basic protein and Charcot-Leyden crystal protein in human tears[J]. Am J Ophthalmol, 1981, 92(6): 824-828. doi:10.1016/s0002-9394(14)75637-5.
[57] Murakami A, Tutumi T, Watanabe K. Middle ear effusion and fungi[J]. Ann Otol Rhinol Laryngol, 2012, 121(9): 609-614. doi:10.1177/000348941212100908.
[58] Lao LM, Kumakiri M, Nakagawa K, et al. The ultrastructural findings of Charcot-Leyden crystals in stroma of mastocytoma[J]. J Dermatol Sci, 1998, 17(3): 198-204. doi:10.1016/s0923-1811(98)00013-9.
[59] Strauchen JA, Gordon RE. Crystalline inclusions in granulocytic sarcoma[J]. Arch Pathol Lab Med, 2002, 126(1): 85-86. doi:10.1043/0003-9985(2002)126<0085:CIIGS>2.0.CO;2.
[60] Khrizman P, Altman JK, Mohtashamian A, et al. Charcot-Leyden crystals associated with acute myeloid leukemia: case report and literature review[J]. Leuk Res, 2010, 34(12): e336-e338. doi:10.1016/j.leukres.2010.08.014.
[61] Kanitakis J. Charcot-Leyden crystals in Pemphigus vegetans[J]. J Cutan Pathol, 1987, 14(2): 127.
[62] Kumar PV, Mousavi A, Karimi M, et al. Fine needle aspiration of Langerhans cell Histiocytosis of the lymph nodes. A report of six cases[J]. Acta Cytol, 2002, 46(4): 753-756. doi:10.1159/000326991
[63] De Re V, Simula M P, Caggiari L, et al. Proteins specifically hyperexpressed in a coeliac disease patient with aberrant T cells[J]. Clin Exp Immunol,2007,148(3):402-409. doi: 10.1111/j.1365-2249.2007.03348.x.
[64] 李华斌,赖玉婷,姜文秀. 慢性鼻窦炎的内表型研究进展及精准治疗[J]. 山东大学耳鼻喉眼学报,2019,33(3):9-13. doi:10.6040/j.issn.1673-3770.1.2018.043 LI Huabin, LAI Yuting, JIANG Wenxiu. Endotypes and precision medicine in chronic sinusitis treatment[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(3):9-13. doi:10.6040/j.issn.1673-3770.1.2018.043.

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed