山东大学耳鼻喉眼学报 ›› 2016, Vol. 30 ›› Issue (2): 50-55.doi: 10.6040/j.issn.1673-3770.0.2016.064

• 论著 • 上一篇    下一篇

自噬相关基因Atg3、Ambra1与慢性单纯性鼻窦炎、鼻息肉及鼻息肉伴发哮喘的相关性研究

张金陵,蔡晓岚,李学忠,冯昕,齐君君,刘大昱   

  1. 山东大学齐鲁医院耳鼻咽喉头颈外科, 卫生部耳鼻咽喉科学重点实验室, 山东 济南 250012
  • 收稿日期:2016-02-19 出版日期:2016-04-16 发布日期:2016-04-16
  • 通讯作者: 李学忠. E-mail:lxzebyh@163.com; 蔡晓岚. E-mail:cxlsd@sdu.edu.cn E-mail:zhangjinling120@sina.co
  • 作者简介:张金陵. E-mail:zhangjinling120@sina.co
  • 基金资助:
    山东省自然科学基金资助项目(ZR2014HM006);山东大学齐鲁医院科研基金资助项目(2015QLMS25);山东大学基本科研业务费专项资金资助(2015QLMS25)

Study of autophagy-related genes atg3, ambra1 expressions in chronic rhinosinusitis without or with nasal polyps accompanied with asthma.

ZHANG Jinling, CAI Xiaolan, LI Xuezhong, FENG Xin, QI Junjun, LIU Dayu   

  1. Department of Otorhinolaryngology, Head and Neck Surgery, Qilu Hospital of Shandong University, Key Laboratory of Otolaryngology of Health Ministry, Shandong University, Jinan 250012, Shandong, China
  • Received:2016-02-19 Online:2016-04-16 Published:2016-04-16

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摘要： 目的 自噬是机体的一种重要的保护和防御机制。研究拟通过对自噬相关基因Atg3、Ambra1表达的观察,探讨自噬与慢性鼻窦炎(CRS)及哮喘之间的关联性及可能的作用机制。 方法 收集24例鼻中隔偏曲患者的下鼻甲黏膜作为正常对照组,选取21例慢性单纯性鼻窦炎患者(CRSsNP组)、22例慢性鼻窦炎鼻息肉患者(CRSwNP组)及18例慢性鼻窦炎鼻息肉伴发哮喘患者(CRSwNP伴哮喘组)的鼻腔黏膜组织为实验组。采用免疫组织化学染色技术检测Atg3、Ambra1在各组中的表达强度及分布,分析自噬相关基因在各组间表达的差异,并分析各组中两基因之间的相关性。 结果 (1) 自噬相关基因Atg3在对照组、CRSsNP组、CRSwNP组、CRSwNP伴哮喘组中表达逐渐增强,在各组间表达差异有统计学意义(χ2=31.080, P<0.001);进一步两两比较,在对照组与CRSsNP组之间(P=0.002)、对照组与CRSwNP组之间(P<0.001)、对照组与CRSwNP伴哮喘组之间(P<0.001)、CRSsNP组与CRSwNP伴哮喘组之间(P=0.002)、CRSwNP组与CRSwNP伴哮喘组之间(P=0.024)表达差异均有统计学意义,在CRSsNP组与CRSwNP组之间(P=0.304)表达差异无统计学意义。(2) 自噬相关基因Ambra1在对照组、CRSsNP组、CRSwNP组、CRSwNP伴哮喘组中表达逐渐增强,在各组间表达差异有统计学意义(χ2=33.000, P<0.001);进一步两两比较,在对照组与CRSsNP组之间(P=0.009)、对照组与CRSwNP组之间(P<0.001)、对照组与CRSwNP伴哮喘组之间(P<0.001)、CRSsNP组与CRSwNP伴哮喘组之间(P<0.001)、CRSwNP组与CRSwNP伴哮喘组之间(P=0.009)表达差异均有统计学意义,在CRSsNP组与CRSwNP组之间(P=0.205)表达差异无统计学意义。(3)8. Atg3与Ambra1在CRSsNP组(r=0.619, P=0.003)、CRSwNP组(r=0.392, P=0.022)和CRSwNP伴哮喘组(r=0.552, P=0.033)中表达呈正相关性,在对照组中无相关性(r=0.316, P=0.133)。 结论 细胞自噬与CRS疾病的发生发展密切相关,可能参与了CRSwNP伴哮喘的发生,且自噬相关基因Atg3与Ambra1在CRS的发生发展过程中可能存在协同作用,自噬可成为CRS诊疗新的研究方向。

关键词: Ambra1, 自噬, 哮喘, Atg3, 慢性鼻窦炎

Abstract: Objective Autophagy was an important mechanism of protection and defense in organisms. The purpose of the study was observed the expressions of autophagy-related genes Atg3 and Ambra1, investigated the relevance between autophagy, chronic rhinosinusitis(CRS)without or with nasal polyps accompanied with asthma and discussed the probable mechanisms. Methods Collection the mucosa membrane tissue of inferior turbinate in patients with nasal septum deviation(n=24)as control group, research 3 groups included mucous in ostiomeatal complex with chronic rhinosinusitis without nasal polyps(CRSsNP)(n=21), chronic rhinosinusitis with nasal polyps(CRSwNP)(n=22)and chronic rhinosinusitis with nasal polyps accompanied with asthma(CRSwNP accompanied with asthma)(n=18). The expressions of Ambra1 and Atg3 in each group were detected by Immunohistochemistry. Relevance of the autophagy-related 山东大学耳鼻喉眼学报30卷2期 -张金陵,等.自噬相关基因Atg3、Ambra1与慢性单纯性鼻窦炎、鼻息肉及鼻息肉伴发哮喘的相关性研究 \=-genes among the groups was analyzed. Results (1)The expressions of autophagy-related genes Atg3 in control group, CRSsNP group, CRSwNP group and CRSwNP accompanied with asthma group were increased gradually(χ2=31.080, P<0.001). Further pairwise comparisons in the control group with CRSsNP group(P=0.002), the control group and CRSwNP group(P<0.001), control group and CRSwNP accompanied with asthma group(P<0.001), CRSsNP group and CRSwNP accompanied with asthma group(P=0.002), CRSwNP and CRSwNP accompanied with asthma group(P=0.024)expression were statistically significant differences. However, there was not any statistically significant difference between CRSsNP group and CRSwNP group(P=0.304).(2)The expressions of autophagy-related genes Ambra1 in control group, CRSsNP group, CRSwNP group and CRSwNP accompanied with asthma group were all gradually increased(χ2=33.000, P<0.001). Further pairwise comparisons in the control group with CRSsNP group(P=0.009), control group and CRSwNP group(P<0.001), control group and CRSwNP accompanied with asthma group(P<0.001), CRSsNP group and CRSwNP accompanied with asthma group(P<0.001), CRSwNP group and CRSwNP accompanied with asthma group(P=0.009)expression were statistically significant difference, There was not any statistically significant difference between CRSsNP group and CRSwNP group(P=0.205).(3)The expressions of Atg3 and Ambra1 were positively correlated in CRSsNP group(r=0.619, P=0.003), CRSwNP group(r=0.392, P=0.022)and CRSwNP accompanied with asthma group(r=0.552, P= 0.033)(P<0.05). However, there was no statistical correlation between Atg3 and Ambra1 in the contrast group(r=0.316, P=0.133)(P>0.05). Conclusion Autophagy is closely related with the CRS pathogenesis and may be involved in the occurrence of CRSwNP accompanied with asthma. Autophagy related genes Atg3 and Ambra1 may play a synergistic role in the development of CRS. Autophagy can be considered as a potential target for the diagnosis and therapeutic treatment in CRS.

Key words: Chronic rhinosinusitis, Ambra1, Autophagy, Atg3, Asthma

中图分类号: 

  • R765.2
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