山东大学耳鼻喉眼学报 ›› 2016, Vol. 30 ›› Issue (3): 52-55.doi: 10.6040/j.issn.1673-3770.0.2015.308

• 论著 • 上一篇    下一篇

异种(牛)脱细胞真皮基质-游离空肠复合体构建实验研究

常江沂,马玲国,李建裕,周敬淳,周舟,曾凡倩,徐杨   

  1. 深圳市人民医院/暨南大学第二附属医院耳鼻咽喉头颈外科, 广东 深圳 518020
  • 收稿日期:2015-07-26 出版日期:2016-06-16 发布日期:2016-06-16
  • 通讯作者: 马玲国. E-mail:ljymax@163.com E-mail:changjhiangyi2015@sohu.co
  • 作者简介:常江沂. E-mail:changjhiangyi2015@sohu.co
  • 基金资助:
    深圳市科技计划项目(201202131)

Experimental study on the construction of heterogeneous decellularized dermal matrix-free jejunal complex.

CHANG Jiangyi, MA Lingguo, LI Jianyu, ZHOU Jingchun, ZHOU Zhou, ZENG Fanqian, XU Yang   

  1. Department of Otolaryngology &Head and Neck Surgery, Shenzhen Peoples Hospital/Second Clinical College of Jinan University, Shenzhen 518020, Guangdong, China
  • Received:2015-07-26 Online:2016-06-16 Published:2016-06-16

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摘要： 目的 研究异种(牛)脱细胞真皮基质-游离空肠复合体的构建技术,以期解决游离空肠移植重建长段气管术后肠腺分泌的问题。 方法 选择10只杂种犬,制备去上皮游离空肠段,将剪成合适大小的异种(牛)脱细胞真皮基质覆盖于游离空肠基底膜表面,并用覆膜镍钛合金支架支撑固定,将构建好的复合体移至腹膜外与腹部皮肤之间,分别于术后7 d、14 d、1个月、3个月和6个月后取出,查看复合体的生长情况。 结果 10只实验犬中,有1只因肠粘连和伤口感染死亡而未能完成实验,余9只实验犬术后存活良好。术后7 d、14 d肉眼观察和病理检查,在游离空肠内表面均见有异种(牛)脱细胞真皮基质成分,表面黏膜层可见部分鳞状上皮化生;术后1个月、3个月、6个月肉眼和病理检查均未再发现异种(牛)脱细胞真皮基质成分,游离空肠内表面几乎全部化生为复层鳞状上皮。 结论 合适大小的异种(牛)脱细胞真皮基质覆盖在去上皮游离空肠内表面,可以促进游离空肠内表面的鳞状上皮化生,抑制肠腺分泌。覆膜镍钛记忆合金支架有一定的弹性,可以很好的对异种(牛)脱细胞真皮基质-去上皮游离空肠复合体起到支撑固定作用。

关键词: 气管重建, 动物实验, 游离空肠, 异种(牛)脱细胞真皮基质, 抑制, 肠腺分泌

Abstract: Objective To study the construction of heterogeneous decellularized dermal matrix-free jejunal complex, in order to solve problem of intestinal secretion after the reconstruction of long-segment trachea with free jejunal graft. Methods A total of 10 adult mongrel dogs were involved. We cut the heterogeneous decellularized dermal matrix into suitable diameter, put it on the inner face of the jejunum whose mucous layer had been removed, and then used the Shape-memory Titanium-nickel Alloy stand to support it. On day 7, 14, 30, 90 and 180, we harvested the complex to observe the growth of epithelium with microscope. Results Of all 10 dogs, one dog died of intestinal adhesion and wound infection. The other 9 dogs lived to the end of the experiment. On day 7 and 14, we detected heterogeneous decellularized dermal matrix components, and part of free jejunal inner surface was squamous metaplasia. On day 30, 90 and 180, we did not detect any heterogeneous decellularized dermal matrix components, and almost all free jejunal inner surface was squamous metaplasia. Conclusion Heterogeneous decellularized dermal matrix with appropriate size could survive on the inner surface of the jejunum whose mucous layer had been removed and promote squamous metaplasia, thus inhibiting intestinal secretion. The Shape-memory Titanium-nickel Alloy stand could serve as a good support.

Key words: Intestinal secretion, Heterogeneous decellularized dermal matrix, Inhibition, Animal experiment, Free jejunum, Tracheal reconstruction

中图分类号: 

  • R739.6
[1] 寇秀娟, 陈小红. 长段气管缺损后重建的研究进展[J]. 国际耳鼻咽喉头颈外科杂志, 2012, 39(5):266-269. KOU Xiujuan, CHEN Xiaohong. The research progress of reconstruction of long tracheal defect[J]. Int J Otolaryngology Head Neck Surgery, 2012, 39(5):266-269.
[2] Du X F, Kwon S K, Song J J, et al. Tracheal reconstruction by mesenchymal stem cells with small intestine submucosa in rabbits [J]. Int J Pediatr Otorhinolaryngol, 2012, 76(3):345-351.
[3] 马玲国, 王跃建, 何发尧, 等. 记忆合金支架与游离空肠移植联合重建长段气管动物实验研究[J]. 中华耳鼻咽喉科杂志, 2004, 39(10):612-614. MA Lingguo, WANG Yuejian, HE Fayao, et al. Experimental reconstruction of the canine trachea with shape-memory titanium-nickel alloy stent coupled with free jejunal graft [J]. Chin J Otorhinolarynol, 2004, 39(10):612-614.
[4] 郑德育, 崔鹏程, 罗家胜, 等. 游离空肠复合镍钛合金网重建犬气管的实验研究[J]. 第四军医大学学报, 2008, 29(20):1707-1710. ZHENG Deyu, CUI Pengcheng, LUO Jiasheng, et al. Experimental reconstruction of the canine trachea with free jejunum coupled with nickel titanium mesh[J]. J Fourth Military Med Univ, 2008, 29(20):1707-1710.
[5] Nakayama M. Experimental reconstruction of the trachea with free jejunal graft [J]. Nippon Kyobu Ceka Cakkai zasshi, 1990, 38:1429-1435.
[6] 陈恩宽, 王跃建, 宋文光, 等. 去部分黏膜游离空肠重建气管缺损肠黏膜组织学变化研究[J]. 山东大学耳鼻喉眼学报, 2011, 25(1):72-76. CHEN Enkuan, WANG Yuejian, SONG Wenguang, et al. Histological research of tracheal reconstruction by free jejunal graft with the mucosa partially preserved[J]. J Otolaryngol Ophthalmol Shandong Univ, 2011, 25(1):72-76.
[7] Rainer C, Wechselberger G, Bauer T, et al. Tansplantation of tracheal epithelial cells onto a prefabricated capsule pouch with fibrin glue as a delivery vehicle[J]. J Thorac Cardiovasc Surg, 2001, 121(June): 1187-1193.
[8] 徐辉, 王岩. 镍钛记忆合金棒用于脊柱畸形的研究进展[J]. 脊柱外科杂志, 2014, 12(2):120-122. XU Hui, WANG Yan. The research progress of Nickel-titanium memory alloy rods used in of spinal deformity [J]. J Spinal Surgery, 2014, 12(2):120-122.
[9] Artins J, Silva H, Certal V, et al. Ossiculo plasty with titanium prosthesis [J]. Acta Oto Mnolaringol Esp, 2011, 62(10):295-299.
[10] 孙岩, 张庆泉, 张华, 等. 异种(牛)脱细胞真皮基质修复膜在耳鼻咽喉头颈外科术后缺损修复中的应用[J]. 山东大学耳鼻喉眼学报, 2008, 22(4):316-319. SUN Yan, ZHANG Qingquan, ZHANG Hua, et al. Heterogenerty(cattle)acellular dermal matrix repairing mucosa in otolaryngology reconstruction surgeries [J]. J Otolaryngol Ophthalmol Shandong Univ, 2008, 22(4):316-319.
[11] 任烨, 邓辰亮, 杨松林, 等. 脱细胞真皮基质在整形外科领域的研究及应用进展[J]. 中国美容整形外科杂志, 2014, 25(8):474-478. REN Ye, DENG Chenliang, YANG Songlin, et al. The research and application progress of acellular dermal matrix in the field of plastic surgery [J]. Chin J Aesthetic Plastic Surgey, 2014, 25(8):474-478.
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