双氢青蒿素对下咽癌Fadu细胞内质网应激途径的作用

doi:10.6040/j.issn.1673-3770.1.2019.031

摘要/Abstract

摘要： 目的

探讨双氢青蒿素(DHA)抑制下咽癌Fadu细胞增殖的机制，为其应用于临床抗肿瘤治疗提供理论依据。

方法

应用含不同浓度葡萄糖(5.5、10、15、25、50、100 mM)培养基，培养Fadu细胞36 h，Western blot检测p-STAT3及内质网应激相关蛋白GRP78的表达情况。应用不同浓度(10、20、40、80、160 μM)DHA在培养基糖浓度为25 mM条件下处理下咽癌Fadu细胞，24 h后用MTT比色法检测对细胞增殖的影响；Western blot检测p-STAT3、GRP78的表达情况。

结果

DHA抑制下咽癌Fadu细胞增殖，且具剂量依赖性；低糖培养基可以降低p-STAT3的表达，增加GRP78的表达；DHA抑制p-STAT3表达，低浓度DHA刺激GRP78表达，随着浓度逐渐增高则抑制其表达。

结论

DHA可以显著抑制下咽癌Fadu细胞的增殖，抑制p-STAT3的表达，诱导内质网应激，其作用机制可能与干扰细胞糖代谢密切相关。

关键词: 双氢青蒿素, 下咽癌, Fadu细胞, 内质网应激

Abstract: Objective

To investigate the mechanism of inhibition by dihydroartemisinin (DHA) of the proliferation of FaDu cells in hypopharyngeal cancer, and provide a theoretical basis for its clinical application in anti-tumor therapy.

Methods

FaDu cells were cultured in different concentrations of glucose (5.5, 10, 15, 25, 50, 100 mM) for 36 h, then the expression of p-STAT3 and endoplasmic reticulum stress-related protein GRP78 were detected by western blot. FaDu cells of hypopharyngeal carcinoma were treated with DHA at different concentrations (10, 20, 40, 80, 160 M) in a culture medium with 25 mM glucose, and the effect on cell proliferation was detected by MTT assay 24 h later. Western blot was used to detect the expression of p-STAT3 and GRP78.

Results

DHA inhibited the proliferation of FaDu hypopharyngeal carcinoma cells in a concentration-dependent manner. Low-glucose concentration medium reduced the expression of p-STAT3 and increased the expression of GRP78. DHA inhibited the expression of p-STAT3; GRP78 expression was stimulated at low DHA concentrations, but was inhibited as the DHA concentration increased.

Conclusions

DHA significantly inhibits the proliferation of FaDu hypopharyngeal carcinoma cells, inhibits the expression of p-STAT3, and induces endoplasmic reticulum stress, which may be closely related to the interference of cellular glucose metabolism.

Key words: Dihydroartemisinin, Hypopharyngeal cance, Fadu cells, Endoreticulum stress

中图分类号:

R739.6

黄泽雷,宋琦,路秀英,崔兰珍,李晓明. 双氢青蒿素对下咽癌Fadu细胞内质网应激途径的作用[J]. 山东大学耳鼻喉眼学报, 2019, 33(4): 31-35.

Zelei HUANG,Qi SONG,Xiuying LU,Lanzhen CUI,Xiaoming LI. Effects of dihydroartemisinin on endoreticulum stress pathway in FaDu hypopharyngeal carcinoma cells[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(4): 31-35.

图/表 3

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