山东大学耳鼻喉眼学报 ›› 2020, Vol. 34 ›› Issue (6): 72-76.doi: 10.6040/j.issn.1673-3770.0.2019.618

• • 上一篇    下一篇

CPS1在喉鳞癌组织中的表达及临床意义

范义燕1,2,张肖林1,刘秀珍3,尹晶晶1,袁进1,王延飞1,陈军1   

  1. 1. 滨州医学院附属医院 耳鼻咽喉头颈外科, 山东 滨州 256603;
    2. 胜利油田中心医院 耳鼻喉科, 山东 东营 257000;
    3. 滨州医学院附属医院 临床医学实验室, 山东 滨州 256603
  • 发布日期:2021-01-11
  • 通讯作者: 陈军. E-mail:bzchenjun@126.com

Expression and clinical significance of CPS1 in laryngeal squamous cell carcinoma

FAN Yiyan1,2, ZHANG Xiaolin1, LIU Xiuzhen3, YIN Jingjing1, YUAN Jin1, WANG Yanfei1, CHEN Jun1   

  1. 1. Otorhinolaryngology & Head and Neck Surgery, Binzhou Medical University Hospital, Binzhou 256603, Shandong, China;
    2. Department of Otolaryngology, Shengli Oilfield Central Hospital, Dongying 257000, Shandong, China;
    3. Clinical Medical Laboratory, Binzhou Medical University Hospital, Binzhou 256603, Shandong, China
  • Published:2021-01-11

PDF (PC)

211

摘要： 目的 研究氨基甲酰磷酸合成酶1(CPS1)在喉鳞癌患者组织中的表达水平及与临床病理指标的相关性,探讨CPS1对喉鳞癌的临床意义。 方法 采用逆转录实时定量聚合酶链反应(RT-qPCR)及Western blot免疫印迹技术,分别检测喉鳞癌组织及癌旁组织中CPS1 mRNA及蛋白表达水平,同时采用免疫组织化学法检测 CPS1在喉鳞癌组织中的表达水平及其结构中的分布情况。 结果 RT-qPCR 和Western blot结果显示,与癌旁组织相比,喉鳞癌组织CPS1 mRNA 和蛋白表达明显增高,表达差异有统计学意义(P<0.01);免疫组化结果显示CPS1表达与肿瘤分化程度、临床分期、淋巴结转移等因素密切相关(P<0.05),而与患者年龄、性别、吸烟史、饮酒史及肿瘤部位无关(P>0.05)。 结论 在喉鳞癌组织中CPS1的mRNA和蛋白表达明显升高,且与临床进展密切相关,说明CPS1在喉癌发生及转移过程中起到一定的作用,可能作为喉癌诊断的重要指标。

关键词: 氨基甲酰磷酸合成酶1, 喉鳞状细胞癌, 临床进展

Abstract: Objective To study the expression levels of carbamoyl phosphate synthase I(CPS1)in laryngeal squamous cell carcinoma and adjacent non-cancerous tissues and reveal its possible correlation with clinical parameters, and explore the clinical significance of CPS1 in the disease. Methods The real-time quantitative polymerase chain reaction(RT-qPCR)was used to measure the expression of CPS1 mRNA in laryngeal squamous cell carcinoma and adjacent tissues. Western blotting and immunohistochemistry were used to detect expression levels of the CPS1 protein and its distribution in laryngeal squamous cell carcinoma tissues. Results The results of RT-qPCR and western blot analysis showed that the mRNA and protein expression levels of CPS1 in laryngeal squamous cell carcinoma were significantly higher than that in adjacent tissues(P<0.01). Immunohistochemistry showed the same trend in CPS1 protein levels. The expression of CPS1 was closely related to the degree of tumor differentiation, clinical stage, and lymph node metastasis(P<0.05), but not to the patients' age, gender, smoking history, drinking history, and tumor site(P>0.05). Conclusion The expression of CPS1 in laryngeal squamous cell carcinoma is significantly higher than in the adjacent tissues, and is closely related to clinical progression. These results suggest that CPS1 may play a role in the occurrence and metastasis of laryngeal cancer, and is a diagnostic indicator for laryngeal cancer.

Key words: Carbamyl phosphate synthase 1, Laryngeal squamous cell carcinoma, Clinical progress

中图分类号: 

  • R739.65
[1] Zhu L, Qin G, Ye L, et al. Epithelial cell transforming sequence 2 expression is associated with the progression of laryngeal squamous cell carcinoma[J]. Oncol Lett, 2019, 17(6): 5699-5704. doi:10.3892/ol.2019.10226.
[2] 邹良玉,李连贺,岳文慧,等. MIF、GSK-3β在喉鳞状细胞癌中表达的临床意义及相关性研究[J]. 山东大学耳鼻喉眼学报, 2019, 33(2): 76-80. doi:10.6040/j.issn.1673-3770.0.2018.423. ZOU Liangyu, LI Lianhe, YUE Wenhui, et al. Clinical significance and correlation between MIF and GSK-3βexpression in laryngeal squamous cell carcinoma[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(2): 76-80. doi:10.6040/j.issn.1673-3770.0.2018.423.
[3] 许莉, 王志颐, 陈伟,等. MicroRNA-24对人喉鳞癌细胞生物学行为的影响[J]. 山东大学耳鼻喉眼学报, 2018, 32(6): 30-37. doi:10.6040/j.issn.1673-3770.0.2018.021. XU Li, WANG Zhiyi, CHEN Wei, et al. Effect of MicroRNA-24 on the proliferation, migration, invasion, and apoptosis of LSCC cells[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2018, 32(6): 30-37. doi:10.6040/j.issn.1673-3770.0.2018.021.
[4] Markou K, Christoforidou A, Karasmanis I, et al. Laryngeal cancer: epidemiological data from Νorthern Greece and review of the literature[J]. Hippokratia, 2013, 17(4): 313-318.
[5] Yan ZY, Liu G, Liang M, et al. Ophiopogonin D inhibits cell proliferation and induces apoptosis of human laryngocarcinoma through downregulation of cyclin B1 and MMP-9 and upregulation of p38-MAPK signaling[J]. Oncol Lett, 2019, 17(2): 1877-1882. doi:10.3892/ol.2018.9788.
[6] Zhang B, Fu T, Zhang L. MicroRNA-153 suppresses human laryngeal squamous cell carcinoma migration and invasion by targeting the SNAI1 gene[J]. Oncol Lett, 2018, 16(4): 5075-5083. doi:10.3892/ol.2018.9302.
[7] Zhuang S, Liu F, Wu P. Upregulation of long noncoding RNA TUG1 contributes to the development of laryngocarcinoma by targeting miR-145-5p/ROCK1 axis[J]. J Cell Biochem, 2019, 120(8): 13392-13402. doi:10.1002/jcb.28614.
[8] Butler SL, Dong H, Cardona D, et al. The antigen for Hep Par 1 antibody is the urea cycle enzyme carbamoyl phosphate synthetase 1[J]. Lab Invest, 2008, 88(1): 78-88. doi:10.1038/labinvest.3700699.
[9] Diez-Fernandez C, Martínez AI, Pekkala S, et al. Molecular characterization of carbamoyl-phosphate synthetase(CPS1)deficiency using human recombinant CPS1 as a key tool[J]. Hum Mutat, 2013, 34(8): 1149-1159. doi:10.1002/humu.22349.
[10] Yang C, Fu R, Zhuang ZH, et al. Studies on the biological functions of CPS1 in AFB1 induced hepatocarcinogenesis[J]. Gene, 2016, 591(1): 255-261. doi:10.1016/j.gene.2016.07.031.
[11] Chu PG, Weiss LM. Immunohistochemical characterization of signet-ring cell carcinomas of the stomach, breast, and colon[J]. Am J Clin Pathol, 2004, 121(6): 884-892. doi:10.1309/A09E-RYMF-R64N-ERDW.
[12] Thamboo TP, Wee A. Hep Par 1 expression in carcinoma of the cervix: implications for diagnosis and prognosis[J]. J Clin Pathol, 2004, 57(1): 48-53. doi:10.1136/jcp.57.1.48.
[13] Palaniappan A, Ramar K, Ramalingam S. Computational identification of novel stage-specific biomarkers in colorectal cancer progression[J]. PLoS One, 2016, 11(5): e0156665. doi:10.1371/journal.pone.0156665.
[14] Daniel P, Halada P, Jelínek M, et al. Differentially expressed mitochondrial proteins in human MCF7 breast cancer cells resistant to paclitaxel[J]. Int J Mol Sci, 2019, 20(12): E2986. doi:10.3390/ijms20122986.
[15] Çeliktas M, Tanaka I, Tripathi SC, et al. Role of CPS1 in cell growth, metabolism and prognosis in LKB1-inactivated lung adenocarcinoma[J]. J Natl Cancer Inst, 2017, 109(3): 1-9. doi:10.1093/jnci/djw231.
[16] Li J, Chen L, Zhang XF, et al. Detection of circulating tumor cells in hepatocellular carcinoma using antibodies against asialoglycoprotein receptor, carbamoyl phosphate synthetase 1 and Pan-cytokeratin[J]. PLoS One, 2014, 9(4): e96185. doi:10.1371/journal.pone.0096185.
[17] Milinkovic V, Bankovic J, Rakic M, et al. Identification of novel genetic alterations in samples of malignant glioma patients[J]. PLoS One, 2013, 8(12): e82108. doi:10.1371/journal.pone.0082108.
[18] Kinoshita M, Miyata M. Underexpression of mRNA in human hepatocellular carcinoma focusing on eight loci[J]. Hepatology, 2002, 36(2): 433-438. doi:10.1053/jhep.2002.34851.
[19] Liu HY, Dong HJ, Robertson K, et al. DNA methylation suppresses expression of the urea cycle enzyme carbamoyl phosphate synthetase 1(CPS1)in human hepatocellular carcinoma[J]. Am J Pathol, 2011, 178(2): 652-661. doi:10.1016/j.ajpath.2010.10.023.
[20] Lee YY, Li CF, Lin CY, et al. Overexpression of CPS1 is an independent negative prognosticator in rectal cancers receiving concurrent chemoradiotherapy[J]. Tumour Biol, 2014, 35(11): 11097-11105. doi:10.1007/s13277-014-2425-8.
[21] Liu X, Zhang XT, Bi JB, et al. Caspase recruitment domain family member 10 regulates carbamoyl phosphate synthase 1 and promotes cancer growth in bladder cancer cells[J]. J Cell Mol Med, 2019, 23(12): 8128-8138. doi:10.1111/jcmm.14683.
[22] Li L, Mao YX, Zhao LN, et al. p53 regulation of ammonia metabolism through urea cycle controls polyamine biosynthesis[J]. Nature, 2019, 567(7747): 253-256. doi:10.1038/s41586-019-0996-7.
[23] 刘义文, 廖春娇, 黄顺东. 肝癌组织中SIRT5的表达及其临床病理意义[J]. 中国医学创新, 2019, 16(4): 136-139. doi:10.3969/j.issn.1674-4985.2019.04.035. LIU Yiwen, LIAO Chunjiao, HUANG Shundong. The expressions and clinicopathological significance of SIRT5 in hepatocellular carcinoma[J]. Medical Innovation of China, 2019, 16(4): 136-139. doi:10.3969/j.issn.1674-4985.2019.04.035.
[24] 李佳峰, 孙宇哲, 李庆昌. Sirtuin 4和Sirtuin 5蛋白在代谢性疾病与肿瘤中的研究进展[J]. 现代肿瘤医学, 2018, 26(21): 3516-3519. doi:10.3969/j.issn.1672-4992.2018.21.043. LI Jiafeng, SUN Yuzhe, LI Qingchang. Progress in the research of Sirtuin 4/5 proteins in metabolic diseases and tumors: a review[J]. Journal of Modern Oncology, 2018, 26(21): 3516-3519. doi:10.3969/j.issn.1672-4992.2018.21.043.
[25] Cardona DM, Zhang XK, Liu C. Loss of carbamoyl phosphate synthetase I in small-intestinal adenocarcinoma[J]. Am J Clin Pathol, 2009, 132(6): 877-882. doi:10.1309/ajcp74xgrfwtflju.
[1] 刘勇,袁存立,曹慧,郑成彩,晁方, 许风雷. CHD1L通过EMT促进喉鳞状细胞癌细胞的增殖、侵袭和转移[J]. 山东大学耳鼻喉眼学报, 2022, 36(2): 32-39.
[2] 陈慧君,宋圣花,董伟达,周涵. 术前外周血纤维蛋白原水平对喉癌预后的影响[J]. 山东大学耳鼻喉眼学报, 2020, 34(2): 110-114.
[3] 朱晓城,钱晓云,顾亚军,沈晓辉,宋盼盼,李惠,高下. MGMT和EGFR蛋白在喉鳞状细胞癌中的表达及临床意义[J]. 山东大学耳鼻喉眼学报, 2017, 31(4): 68-72.
[4] 安永明,穆文利,田艳勋,李伟,吴彦桥. 喉鳞癌组织中抑癌基因Maspin和转录因子Ets1的表达及意义[J]. 山东大学耳鼻喉眼学报, 2017, 31(3): 91-94.
[5] 李富, 赵书佑. 肺癌肿瘤抑制因子-1在喉鳞癌组织中的表达及其意义[J]. 山东大学耳鼻喉眼学报, 2015, 29(6): 36-38.
[6] 许海艳1，彭解人1，区永康1，张琼霞2，张存良1，汤智平1，关中1. 喉癌组织中核表皮生长因子受体与Aurora-A激酶的表达及其临床意义[J]. 山东大学耳鼻喉眼学报, 2012, 26(5): 16-21.
[7] 吴桂卿1，田军2，孙静1，陈琪1. Tiam1和MTA1在喉鳞癌组织中的表达及意义[J]. 山东大学耳鼻喉眼学报, 2012, 26(1): 41-43.
[8] 刘明秋1，赵书佑2. KAI1基因在喉癌及转移淋巴结中的表达[J]. 山东大学耳鼻喉眼学报, 2010, 24(2): 11-13.
[9] 徐志坚,彭解人,黄晓明,关 中,祝晓芬,廖艳萍 . G3BP在喉鳞状细胞癌的表达及其临床意义[J]. 山东大学耳鼻喉眼学报, 2008, 22(5): 401-404 .
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] 林彬,王挥戈 . 功能性内镜鼻窦手术后鼻黏膜纤毛转归的研究[J]. 山东大学耳鼻喉眼学报, 2006, 20(6): 481 -487 .
[2] 公 蕾,孙 洁,薛子超,李敬华,薛卫国 . 鼻腔鼻窦恶性肿瘤细胞周期的DNA分析[J]. 山东大学耳鼻喉眼学报, 2008, 22(3): 193 -195 .
[3] 陈文文 . 1例T/NK淋巴瘤17年演进[J]. 山东大学耳鼻喉眼学报, 2006, 20(5): 472 -472 .
[4] 栾建刚,梁传余,文艳君,李炯 . 抑制表皮生长因子受体基因表达的pSIREN-ShuttleRNAi表达载体的构建[J]. 山东大学耳鼻喉眼学报, 2006, 20(1): 4 -8 .
[5] 马敬, 钟翠萍 . 手术治疗侵犯翼腭窝的鼻咽纤维血管瘤的方法（附5例报告）[J]. 山东大学耳鼻喉眼学报, 2006, 20(1): 30 -32 .
[6] 刘强和,罗香林,耿宛平,陈 晨,雷 迅,刘芳贤,邓 明 . 快速老化小鼠的听功能和耳蜗螺旋神经元的增龄性变化[J]. 山东大学耳鼻喉眼学报, 2008, 22(3): 215 -217 .
[7] 郑鹏凌,陈卫国,易笃友,黄清秀,卢 俊 . 耳内镜下吸引清除耳道耵聍55例并文献复习[J]. 山东大学耳鼻喉眼学报, 2008, 22(3): 223 -226 .
[8] 马 敬,钟翠萍,严 星,安 飞 . 耳屏软骨修补无残余软骨的鼻中隔穿孔15例[J]. 山东大学耳鼻喉眼学报, 2008, 22(3): 246 -247 .
[9] 崔哲洙,严永峰,崔春莲,金顺吉 . 嗜酸性粒细胞在变应性鼻炎合并慢性鼻窦炎的分布特点[J]. 山东大学耳鼻喉眼学报, 2008, 22(3): 250 -252 .
[10] 赵鲁新,翟 洪,潘 洁 . 超声乳化吸除联合晶状体植入治疗急性闭角型青光眼伴白内障23例[J]. 山东大学耳鼻喉眼学报, 2008, 22(3): 260 -262 .
网站版权所有 © 2018 《山东大学耳鼻喉眼学报》编辑部　
地址：山东省济南市山大南路27号（250100）电话：+86-0531-88366912 E-mail: ebhxb@sdu.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发