MAPK、PI3K-AKT、NF-κB在小鼠过敏性鼻炎中的表达及意义

doi:10.6040/j.issn.1673-3770.0.2021.174

摘要/Abstract

摘要： 目的通过构建小鼠过敏性鼻炎模型探讨多信号通路蛋白在过敏性鼻炎发生发展中的机制。方法 40只昆明小鼠随机分为过敏性鼻炎组和对照组(每组20只)。分离两组小鼠头部各8只,4%多聚甲醛固定、石蜡包埋、切片、HE染色法观察小鼠鼻黏膜病理改变、免疫组化检测MAPK、NF-κB、PI3K-AKT信号通路蛋白的表达,24只小鼠鼻黏膜蛋白用于Western blotting分析。结果过敏性鼻炎组小鼠的抓挠、喷嚏、流鼻涕等均远多于对照组,差异具有统计学意义(P<0.05);HE染色过敏性鼻炎组黏膜出现假复层纤毛柱状上皮坏死,上皮结构重构,嗜酸性粒细胞浸润等典型鼻炎病理改变。过敏性鼻炎组三条通路关键蛋白的免疫阳性信号AOD值不同程度的高于对照组(P<0.05)。Western blotting结果显示过敏性鼻炎组与对照组相比MAPK、PI3K-AKT、NF-κB三条信号通路蛋白表达升高,差异有统计学意义(P<0.05)。结论在小鼠过敏性鼻炎发病过程中MAPK、NF-κB、PI3K-AKT三条信号通路蛋白的表达均升高,其激活在小鼠过敏性鼻炎过程中可能存在协同效应。

关键词: 多条信号通路, 过敏性鼻炎, MAPK, AKT, NF-κB

Abstract: Objective To explore the mechanism of multi-signaling pathways proteins in the development of allergic rhinitis(AR)in mice. Methods Forty Kunming mice were randomly divided into allergic rhinitis group(AR group)and control group(Con group)each 20 mice. The nasal of 8 mice in each group was fixed with 4% paraformaldehyde, embedded in paraffin, sectioned and stained by HE method to observe the pathological changes of nasal mucosa. And the expression of MAPK, NF-κB and PI3K-AKT pathways proteins were detected by immunohistochemistry(IHC). The other 24 mice of each group were prepared for Western blot(WB)analysis. Results The behavioral results showed that scratching, sneezing and runny nose of the AR group were far more than Con group(P<0.05). In the HE staining, the mucosa showed pseudostratified ciliated columnar epithelial necrosis, epithelial structural remodeling and eosinophil infiltration etc. typical pathological changes of rhinitis. IHC showed that the positive proteins of three key pathways in the AR group was higher than that of the Con group. The proteins expression of MAPK, PI3K-AKT and NF-κB in the AR group were increased compared with the Con group and had a significant differences by western blot(P<0.05). Conclusion The increased proteins of MAPK, NF-κB and PI3K-AKT signaling pathways may have a synergistic effect on the pathogenesis of AR in mice.

Key words: Multiple signaling pathways, Allergic rhinitis, MAPK, AKT, NF-κB

中图分类号:

R765.21

张雅琪,刘慧敏,曹淋曼,王子钰,林旭,李燕萍,薛刚,吴靖芳. MAPK、PI3K-AKT、NF-κB在小鼠过敏性鼻炎中的表达及意义[J]. 山东大学耳鼻喉眼学报, 2022, 36(3): 254-259.

ZHANG Yaqi, LIU Huimin, CAO Linman, WANG Ziyu, LIN Xu, LI Yanping, XUE Gang, WU Jingfang. Expression and significance of the MAPK,PI3K-AKT,NF-κB pathways of allergic rhinitis in mice[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(3): 254-259.

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