JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2017, Vol. 31 ›› Issue (6): 74-78.doi: 10.6040/j.issn.1673-3770.0.2017.374

Previous Articles     Next Articles

Delayed corneal wound healing is related to impaired IGF-1 expression in diabetic rats.

ZHANG Ying, CHEN Hongmei, WU Xinyi.   

  1. Department of Ophthalmology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2017-09-06 Online:2017-12-16 Published:2017-12-16

PDF (PC)

220

Abstract: Objective To explore the expression of IGF-1 and IGF-1R in corneal wound healing in diabetic rats. Method Male Wistar rats were injected with streptozocin; serum glucose levels and body weight were monitored. Following corneal abrasion, healing rate was evaluated. Real-time PCR and immunofluorescence were used to explore the expression of IGF-1 and IGF-1R. Result Low weights and high glucose levels were observed in diabetic rats. Healing rate was significantly lower in the diabetic rats than in normal rats. mRNA and protein expression of IGF-1 and IGF-1R was significantly lower in the diabetic rat group than in the normal group. Conclusion In diabetic rats, delayed corneal epithelial wound healing might be associated with impaired IGF-1 and IGF-1R expression.

Key words: Diabetes mellitus, Wound healing, Cornea, Insulin-like growth factor-1

CLC Number: 

  • R771
[1] Tripathy K, Chawla R, Sharma YR, et al. Corneal changes in diabetes mellitus[J]. Curr Diabetes Rev, 2012, 8(4):294-302.
[2] Abdelkader H, Patel DV, McGhee CNJ, et al. New therapeutic approaches in the treatment of diabetic keratopathy: a review[J]. Clin Experiment Ophthalmol, 2011, 39(3):259-270.
[3] Chen WL, Lin CT, Ko PS, et al. In vivo confocal microscopic findings of corneal wound healing after corneal epithelial debridement in diabetic vitrectomy[J]. Ophthalmol, 2009, 116(6):1038-1047.
[4] Ko JA, Yanai R, Nishida T. IGF-1 released by corneal epithelial cells induces up-regulation of N-cadherin in corneal fibroblasts[J].J Cell Physiol, 2009, 221(1):254-261.
[5] Robertson DM, Ho SI, Hansen BS, et al. Insulin-like growth factor binding protein-3 expression in the human corneal epithelium[J]. Exp Eye Res, 2007, 85(4):492-501.
[6] Robertson DM, Zhu M, Wu YC. Cellulardistribution of the IGF-1R in corneal epithelial cells[J].Exp Eye Res, 2012, 94(1):179-186.
[7] Ogurtsova K, Rocha Fernandes JD, Huang Y, et al. IDF diabetes atlas: global estimates for the prevalence of diabetes for 2015 and 2040[J]. Diabetes Res Clin Pract, 2017, 128(1):40-50.
[8] Xu K, Yu FS. Impaired epithelial wound healing and EGFR signaling pathways in the corneas ofdiabetic rats[J]. Invest Ophthalmol Vis Sci, 2011, 52(6):3301-3308.
[9] Sun H, Mi X, Gao N, et al. Hyperglycemia-suppressed expression of Serpine1 contributes to delayed epithelialwound healing in diabetic mouse corneas[J]. Invest Ophthalmol Vis Sci, 2015, 56(5):3383-3392.
[10] Yu FS, Yin J, Xu K, et al. Growth factors and corneal epithelial wound healing[J]. Brain Res Bull, 2010, 81(2-3):229-235.
[11] Velander P, Theopold C, Hirsch T, et al. Impaired wound healing in an acute diabetic pig model and the effects of local hyperglycemia[J].Wound Repair Regen, 2008, 16(2):288-293.
[12] Clemmons D, Maile L, Xi G, et al. Igf-1 signaling in response to hyperglycemia and the development of diabetic complications[J]. Curr Diabetes Rev, 2011, 7(4):235-245.
[13] Wang XQ, Lee S, Wilson H, et al. Ganglioside GM3 depletion reverses impaired wound healing in diabetic mice by activating IGF-1 and insulin receptors[J]. J Invest Dermatol, 2014, 134(5):1446-1455.
[14] Hirsch T, Spielmann M, Velander P, et al. Insulin-like growth factor-1 gene therapy and cell transplantation in diabetic wounds[J]. J Gene Med, 2008, 10(11):1247-1252.
[15] Chen QQ, Wang WM. Expression of FGF-2 and IGF-1 in diabetic rats with fracture[J]. Asian Pac J Trop Med, 2014, 7(1):71-75.
[16] Peplow PV, Baxter GD. Gene expression and release of growth factors during delayed wound healing: a review of studies in diabetic animals and possible combined laser phototherapy and growth factor treatment to enhance healing[J]. Photomed Laser Surg, 2012, 30(11):617-636.
[1] YANG Xue, LI Ying. Impact of dry eye on corneal endothelial cell density and morphology [J]. J Otolaryngol Ophthalmol Shandong Univ, 2018, 32(4): 72-76.
[2] YAN Bin, ZHOU Huaisheng, PI Liuqing, DUAN Hucheng, YAN Shigang. Effects of using corneal bandage lens on the cornea after strabismus surgery [J]. J Otolaryngol Ophthalmol Shandong Univ, 2018, 32(4): 87-90.
[3] WANG Lei, ZHANG Han. The calculation of intraocular lens refractive power [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(4): 21-28.
[4] XU Xiao. Clinical observations of Ex-PRESS glaucoma filtration device implantation in patients with glaucoma. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(4): 90-93.
[5] WENG Biyan, LIU Can. Impact of scleral tunnel incision and clear corneal incision on the tear film of patients with cataract after phacoemulsification. [J]. J Otolaryngol Ophthalmol Shandong Univ, 2017, 31(4): 80-83.
[6] LIU Wenjing. Influence of TransPRK and LASEK on postoperative vision, diopter and corneal biomechanical properties in myopic eyes with thin cornea. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(6): 70-74.
[7] LIU Lu, LIANG Hao. Distribution of corneal astigmatism among middle aged and senior citizens with cataract. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(6): 75-77.
[8] SHENG Xia, CHEN Hui, DENG Hong, XIE Qing. Analysis of efficacy of corneal bandage lens after pterygium resection. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(4): 109-112.
[9] LI Dongmei. Influence of long-term use of prostaglandin drugs on meibomian gland function and corneal structure in patients with primary open angle glaucoma. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(3): 89-92.
[10] LIN Li, YANG Xu. Analysis on efficacy of treatment of corneal epithelial dysfunction after cataract surgery. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(2): 84-86.
[11] WANG Dan, WANG Rui, ZHANG Qiming, WANG Liwen. Clinical observation on the effects of three surgical methods for pterygium. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(2): 94-97.
[12] ZHANG Xiao, JIANG Liangzhu, XU Yanyun, MU Guoying. The result of riboflavin/UVA corneal collagen cross-linking with isotonic riboflavin solution and hypotonic riboflavin solution in the treatment of keratoconus. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(2): 87-90.
[13] WANG Junying, LI Xiaofeng. Effect of deproteinized extract of calf blood gel eye on the injury of corneal endothelial cells after nasopharyngeal carcinoma radiotherapy. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(1): 76-79.
[14] SHAO Na, ZHANG Han. Variation of visual acuity and fundus in type 2 diabetic patients after phacoemulsification surgery. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(1): 83-87.
[15] WANG Luping, WU Xinyi. Effect of glycosylated hemoglobin on dry eye in patients with type 2 diabetes mellitus [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2015, 29(6): 71-73.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd