Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2024, Vol. 38 ›› Issue (3): 18-25.doi: 10.6040/j.issn.1673-3770.0.2022.219

• Original Article • Previous Articles    

Expression of abnormal spindle-like microcephaly associated protein in nasopharyngeal carcinoma and its clinical significance

YANG Kaiyan1, TANG Fengzhu2, QIN Qicai1, LI Xuxiang3, FENG Dayi3, NONG Fengjing1, YANG Qiuyun1   

  1. 1. Department of Otorhinolaryngology & Head and Neck Surgery, People's Hospital of Baise, Baise 533000, Guangxi, China2. Department of Otorhinolaryngology & Head and Neck Surgery, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning 530000, Guangxi, China3. Department of Pathology, People's Hospital of Baise, Baise 533000, Guangxi, China
  • Published:2024-06-04

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Abstract: Objective This paper investigated the expression of abnormal spindle-like microcephaly associated protein(ASPM)in nasopharyngeal carcinoma tissue samples using immunohistochemical staining, and further analyzed the relationship between ASPM protein expression and clinicopathological parameters and the prognosis of patients with nasopharyngeal carcinoma, and then discussed the expression of ASPM mRNA in nasopharyngeal carcinoma. Methods The microarray dataset of nasopharyngeal carcinoma was downloaded from the GEO database, and the differential expression of the nasopharyngeal carcinoma microarray dataset was analyzed using R language. The differential expression of ASPM protein in nasopharyngeal carcinoma was analyzed using immunohistochemical staining, the correlation between ASPM expression and clinicopathological parameters of patients with nasopharyngeal carcinoma was analyzed using the chi-square test, the relationship between ASPM expression and prognosis of patients with nasopharyngeal carcinoma was analyzed by survival curves, the risk factors affecting prognosis of patients with nasopharyngeal carcinoma were analyzed using the Cox regression model, and the receiver operator characteristic(ROC)curve was used to evaluate the performance of various prognostic parameters in predicting the prognosis of patients with nasopharyngeal carcinoma. Results ASPM mRNA was found to be highly expressed in nasopharyngeal carcinoma microarray data set through analysis of nasopharyngeal carcinoma microarray data(P<0.001). Immunohistochemical staining showed that expression of ASPM in nasopharyngeal carcinoma tissues was significantly higher than in nasopharyngitis tissues(P<0.001). ASPM expression correlated with T stage(P=0.048), N stage(P=0.050), and total clinical stage(P=0.029). The overall survival time of the high expression ASPM protein group was shorter than that of the low expression ASPM protein group(P<0.001). Multivariate Cox regression model analysis showed that ASPM expression level may be an independent risk factor affecting prognosis of patients with nasopharyngeal carcinoma(P=0.015). ROC curve analysis showed that total clinical stage combined with ASPM expression achieved a higher accuracy in predicting prognosis of patients with nasopharyngeal carcinoma(P<0.001). Conclusion ASPM is highly expressed in nasopharyngeal carcinoma tissue samples, and this high expression is related to malignancy, disease progression, and poor prognosis of nasopharyngeal carcinoma, and may become a valuable biomarker for predicting prognosis in nasopharyngeal carcinoma. Combining total clinical stage and ASPM expression may be a promising method for predicting prognosis of patients with nasopharyngeal carcinoma.

Key words: Nasopharyngeal carcinoma, Immunohistochemistry, Tumor prognosis, Abnormal spindle-like microcephaly associated protein, Differential expression

CLC Number: 

  • R766.3
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