Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2023, Vol. 37 ›› Issue (1): 41-46.doi: 10.6040/j.issn.1673-3770.0.2021.502

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Expression and clinical significance of miR-181b-5P and EPB41L3 protein in laryngeal squamous cell carcinoma

HUANG Hengfeng1,2, MA Kunpeng2, YANG Di1,2, ZHANG Lijun2, ZHANG Shenglin3   

  1. 1. School of Clinical Medicine, Weifang Medical University, Weifang 261031, Shandong, China;
    2. Department of Otorhinolaryngology & Head and Neck Surgery, Affiliated Hospital of Weifang Medical University, Weifang 261031, Shandong, China;
    3. Department of Network Information Center, Weifang Medical University, Weifang 261042, Shandong, China
  • Published:2023-02-06

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Abstract: Objective Currently, the expression relationship and mechanism of miR-181b-5P and Erythrocyte Membrane Protein Band 4.1-like 3(EPB41L3)in laryngeal cancer are still unclear. This paper mainly studied the expression levels of miR-181b-5P and EPB41L3 protein in laryngeal squamous cell carcinoma tissues and adjacent normal tissues, and than conducted correlation analysis. To explore the effects of miR-181b-5P targeted regulation of EPB41L3 on the proliferation, invasion and metastasis of laryngeal neoplasms. Methods Using bioinformatics databases predicted the expression of miR-181b-5P in laryngeal cancer, and quantitative real-time PCR was used to determine the expression of miR-181b-5P in laryngeal squamous cell carcinoma and adjacent normal tissues. The target genes of miR-181b-5P were predicted and analyzed by bioinformatics. The relative expression of EPB41L3 protein was detected by immunohistochemistry. Results The expression of miR-181b-5P in laryngeal carcinoma tissues was significantly higher than that in adjacent normal tissues(P<0.001), and there was significant difference in laryngeal carcinoma tissues with or without lymph node metastasis(P=0.027 3).The expression of EPB41L3 protein in laryngeal carcinoma tissues was significantly lower than that in adjacent normal tissues(P<0.001).The positive expression rate of EPB41L3 protein was obviously different in different pathological differentiation degrees, clinical stages and lymph node metastasis(P<0.05). The expressions of miR-181b-5p and EPB41L3 protein in laryngeal carcinoma tissues were negatively correlated(r=-0.420 8, P=0.002 3). Conclusion miR-181b-5p may negatively regulate the expression of EPB41L3 and than affect the proliferation, invasion and metastasis of laryngeal cancer. This study maybe provide new ideas for clinical targeted therapy of laryngeal cancer.

Key words: Laryngeal cancer, miR-181b-5P, EPB41L3, Immunohistochemistry, Quantitative real-time PCR

CLC Number: 

  • R767.1
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