Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2024, Vol. 38 ›› Issue (3): 1-11.doi: 10.6040/j.issn.1673-3770.0.2023.497

• Original Article •    

Single cell sequencing analysis of RPN2 expression pattern in laryngeal squamous cell carcinoma cells

WU Bin, ZHOU Jingchun   

  1. Department of Otorhinolaryngology, the Second Clinical College of Jinan University, Shenzhen 518020, Guangdong, China
  • Published:2024-06-04

Abstract: Objective This study aims to explore the expression pattern of RPN2 in the microenvironment of laryngeal squamous cell carcinoma at the single-cell level. Methods By acquiring and preprocessing the GSE150321 dataset of laryngeal squamous cell carcinoma single-cell data, dimensionality reduction and clustering algorithms were applied to classify and define cell types, followed by an overview and analysis of RPN2 expression in each cell type. Differential expression genes between RPN2-positive and RPN2-negative tumor cells were screened and subjected to functional enrichment analysis. A cell-cell interaction network was constructed for RPN2-positive and RPN2-negative tumor cells. Results After single-cell sequencing of laryngeal squamous cell carcinoma cells, cells were classified into subgroups such as tumor cells, immune cells, fibroblasts, endothelial cells, and epithelial cells based on cell marker molecules. RPN2 showed relatively higher expression levels in tumor cells and immune cells, while lower expression levels were observed in endothelial cells, fibroblasts, and epithelial cells. Differential expression analysis identified genes associated with RPN2 expression, and functional enrichment analysis revealed that highly expressed genes in RPN2-positive tumor cells were mainly enriched in biological processes such as ribonucleoprotein complex biogenesis and cytoplasmic translation signaling pathways. The constructed cell-cell interaction network demonstrated strong interactions between RPN2-positive tumor cells and fibroblasts, endothelial cells, and immune cells. Conclusion This study reveals the expression pattern of RPN2 in the microenvironment of laryngeal squamous cell carcinoma and the characteristics of RPN2-positive and RPN2-negative tumor cells, as well as their associations with tumor occurrence, development, and prognosis. These findings provide a theoretical basis for the treatment and further research of laryngeal squamous cell carcinoma.

Key words: Laryngeal squamous cell carcinoma, Single-cell sequencing, RibophorinⅡ, Microenvironment, Cell interactome network

CLC Number: 

  • R767.19
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