Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2019, Vol. 33 ›› Issue (3): 56-60.doi: 10.6040/j.issn.1673-3770.1.2019.004

• Original Article • Previous Articles     Next Articles

Differential expression of synaptic vesicle glycoprotein 2A in central auditory system of rats

Pengli LIU,Lu ZHANG,Xiaolu LI()   

  1. Department of Otorhinolaryngology, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, Jiangsu, China
  • Received:2019-01-01 Revised:2019-03-16 Online:2019-05-20 Published:2019-08-07
  • Contact: Xiaolu LI E-mail:lixlnj@qq.com

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Abstract: Objective

To study the differential expression of synaptic vesicle glycoprotein 2A (SV2A) in various subregions of the central auditory system (CAS) of SD rats and to search for region-specific proteins.

Methods

Western blotting and immunohistochemistry were used to detect the expression of SV2A in the inferior colliculus (IC), cochlear nucleus (CN), superior olivary nucleus complex (SOC), and the remaining brain tissues including brain and cerebellum (Rest) of SD rats. One-way analysis of variance was used to compare the differences in protein expression between the groups.

Results

The results of western blotting showed that the expression of SV2A in CN was higher than that in Rest, IC, and SOC, and the differences were statistically significant (P = 0.006, P = 0.019, and P = 0.013, respectively). However, there were no statistically significant differences between IC and Rest (P = 0.802), SOC and Rest (P = 0.928), and IC and SOC (P = 0.99). The immunohistochemistry results also indicated that the expression of SV2A in CN was higher than that in Rest, IC, and SOC, and the differences were statistically significant (P = 0.003, P = 0.016, and P＜0.001, respectively). However, there were no statistically significant differences between IC and Rest (P = 0.94), SOC and Rest (P = 0.863), and IC and SOC (P = 0.536). Therefore, SV2A can be considered as a region-specific protein of CN.

Conclusion

The high expression of SV2A in CN of rats suggests that SV2A may be involved in the processing of auditory information in the CN, which provides a new clue for further exploring the physiological function of CN and the etiology of central auditory processing disorder (CAPD) at the molecular level.

Key words: Synaptic vesicle glycoprotein 2A, Protein, Cochlear nucleus, Central auditory processing

CLC Number: 

  • R764.35

Fig.1

SV2A expression in IC, CN, SOC, and Rest using western blotting"

Fig.2

SV2A expression in different regions of CAS by immunohistochemistry (×400)"

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