JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2016, Vol. 30 ›› Issue (1): 5-8.doi: 10.6040/j.issn.1673-3770.0.2015.320

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Differentially expressed proteins between nasal polyps and normal nasal mucosa by the iTRAQ technology.

ZHANG Jian, LU Xiaoqing, WU Jian.   

  1. Department of Otolaryngology, Shanghai Changzheng Hospital, Shanghai 200003, China
  • Received:2015-08-03 Online:2016-02-16 Published:2016-02-16

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Abstract: Objective To investigate the pathogenic mechanisms of nasal polyp(NP)by analyzing the protein expression profiles of nasal polyps. Methods 4 samples of NP tissues and 4 samples of normal nasal mucosa were retrospectively collected. The protein expression profile of each sample was detected by isobaric tags for relative and absolute quantitation(iTRAQ)technology. Results Out of 311 differentially expressed proteins detected, 88 cases were up-regulated, including Charcot-Leyden crystal protein(CLC)and PLEKHA7. Another 223 proteins were down-regulated, including S100A1 mainly. There were 18 cases and 65 cases of protein, with up-regulated expression more than two times and down-regulated more than 50%, respectively. Conclusion The protein expression profiles vary between NP and normal nasal mucosa up-regulation of CLC may involve in inflammation and immune response of nasal mucosa, and PLEKHA7 may be associated with mucosa reconstruction.

Key words: Nasal mucosa, Nasal polyps, Differentially expressed protein, iTRAQ

CLC Number: 

  • R319
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