Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2019, Vol. 33 ›› Issue (3): 56-60.doi: 10.6040/j.issn.1673-3770.1.2019.004

• Original Article • Previous Articles     Next Articles

Differential expression of synaptic vesicle glycoprotein 2A in central auditory system of rats

Pengli LIU,Lu ZHANG,Xiaolu LI()   

  1. Department of Otorhinolaryngology, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, Jiangsu, China
  • Received:2019-01-01 Revised:2019-03-16 Online:2019-05-20 Published:2019-08-07
  • Contact: Xiaolu LI E-mail:lixlnj@qq.com

RichHTML

3

PDF (PC)

505

Abstract: Objective

To study the differential expression of synaptic vesicle glycoprotein 2A (SV2A) in various subregions of the central auditory system (CAS) of SD rats and to search for region-specific proteins.

Methods

Western blotting and immunohistochemistry were used to detect the expression of SV2A in the inferior colliculus (IC), cochlear nucleus (CN), superior olivary nucleus complex (SOC), and the remaining brain tissues including brain and cerebellum (Rest) of SD rats. One-way analysis of variance was used to compare the differences in protein expression between the groups.

Results

The results of western blotting showed that the expression of SV2A in CN was higher than that in Rest, IC, and SOC, and the differences were statistically significant (P = 0.006, P = 0.019, and P = 0.013, respectively). However, there were no statistically significant differences between IC and Rest (P = 0.802), SOC and Rest (P = 0.928), and IC and SOC (P = 0.99). The immunohistochemistry results also indicated that the expression of SV2A in CN was higher than that in Rest, IC, and SOC, and the differences were statistically significant (P = 0.003, P = 0.016, and P＜0.001, respectively). However, there were no statistically significant differences between IC and Rest (P = 0.94), SOC and Rest (P = 0.863), and IC and SOC (P = 0.536). Therefore, SV2A can be considered as a region-specific protein of CN.

Conclusion

The high expression of SV2A in CN of rats suggests that SV2A may be involved in the processing of auditory information in the CN, which provides a new clue for further exploring the physiological function of CN and the etiology of central auditory processing disorder (CAPD) at the molecular level.

Key words: Synaptic vesicle glycoprotein 2A, Protein, Cochlear nucleus, Central auditory processing

CLC Number: 

  • R764.35

Fig.1

SV2A expression in IC, CN, SOC, and Rest using western blotting"

Fig.2

SV2A expression in different regions of CAS by immunohistochemistry (×400)"

1 DelphiM, ZamiriA F. Dichotic training in children with auditory processing disorder[J]. Int J Pediatr Otorhinolaryngol, 2018, 110: 114-117.doi:10.1016/j.ijporl.2018. 05. 014
doi: 10.1016/j.ijporl.2018. 05. 014
2 李宛桐, 徐莹, 李晓璐, 等. 听觉传导通路下丘区域特异性蛋白分析[J]. 中国耳鼻咽喉头颈外科, 2016, 23(6): 331-334. doi:10.16066/j.1672-7002.2016.06.007.
doi: 10.16066/j.1672-7002.2016.06.007
LIWantong, XUYing, LIXiaolu, et al. Analysis of inferior colliculus region typical proteins in auditory pathway[J]. Chinese Archives of Otolaryngology-Head and Neck Surgery, 2016, 23(6): 331-334. doi:10.16066/j.1672-7002.2016.06.007.
doi: 10.16066/j.1672-7002.2016.06.007
3 徐莹, 李宛桐, 李晓璐. 大鼠上橄榄复合体中枢听觉信息处理相关蛋白的研究[J]. 南京医科大学学报(自然科学版), 2016, 36(9): 1068-1071,1078. doi: 10.7655/NYDXBNS20160909.
doi: 10.7655/NYDXBNS20160909
XUYing, LIWantong, LIXiaolu. A study on the proteins of superior olivary complex related to central auditory processing in SD rats[J]. Acta Universitatis Medicinalis Nanjing, 2016, 36(9): 1068-1071,1078. doi: 10.7655/NYDXBNS20160909.
doi: 10.7655/NYDXBNS20160909
4 KaltwaßerB, SchulenborgT, BeckF, et al. Developmental changes of the protein repertoire in the rat auditory brainstem: a comparative proteomics approach in the superior olivary complex and the inferior colliculus with DIGE and iTRAQ[J]. J Proteomics, 2013, 79: 43-59. doi:10.1016/j.jprot.2012.11.018.
doi: 10.1016/j.jprot.2012.11.018
5 PanneseA, GrandjeanD, FrühholzS. Subcortical processing in auditory communication[J]. Hear Res, 2015, 328: 67-77. doi:10.1016/j.heares.2015.07.003.
doi: 10.1016/j.heares.2015.07.003
6 NothwangHG. Evolution of mammalian sound localization circuits: A developmental perspective[J]. Prog Neurobiol, 2016, 141: 1-24. doi:10.1016/j.pneurobio.2016. 02.003.
doi: 10.1016/j.pneurobio.2016. 02.003
7 RobertsPD, PortforsCV. Responses to social vocalizations in the dorsal cochlear nucleus of mice[J]. Front Syst Neurosci, 2015, 9: 172. doi:10.3389/fnsys.2015.00172.
doi: 10.3389/fnsys.2015.00172
8 SaylesM, StasiakA, WinterIM. Neural segregation of concurrent speech: effects of background noise and reverberation on auditory scene analysis in the ventral cochlear nucleus[J]. Adv Exp Med Biol, 2016, 894: 389-397. doi:10.1007/978-3-319-25474-6_41.
doi: 10.1007/978-3-319-25474-6_41
9 PironeA, KurtS, ZuccottiA, et al. α2δ3 is essential for normal structure and function of auditory nerve synapses and is a novel candidate for auditory processing disorders[J]. J Neurosci, 2014, 34(2): 434-445. doi:10.1523/JNEUROSCI.3085-13.2014.
doi: 10.1523/JNEUROSCI.3085-13.2014
10 张璐, 李晓璐, 李宛桐, 等. Gal-1, ANXA2和PRPH在SD大鼠耳蜗核的表达[J]. 南京医科大学学报(自然科学版), 2017, 37(5): 575-579. doi: CNKI:SUN:NJYK.0.2017-05-012.
doi: CNKI:SUN:NJYK.0.2017-05-012
ZHANGLu, LIXiaolu, LIWantong, et al. Expression of Gal-1, ANXA2 and PRPH in cochlear nucleus on SD rats[J]. Acta Universitatis Medicinalis Nanjing(Natural Science), 2017, 37(5): 575-579. doi: CNKI:SUN:NJYK.0.2017-05-012.
doi: CNKI:SUN:NJYK.0.2017-05-012
11 CrèvecœurJ, FoerchP, DoupagneM, et al. Expression of SV2 isoforms during rodent brain development[J]. BMC Neurosci, 2013, 14: 87. doi:10.1186/1471-2202-14-87.
doi: 10.1186/1471-2202-14-87
12 Lynch BA, MatagneA, BrännströmA, et al. Visualization of SV2A conformations in situ by the use of Protein Tomography[J]. Biochem Biophys Res Commun, 2008, 375(4): 491-495. doi:10.1016/j.bbrc.2008.07.145.
doi: 10.1016/j.bbrc.2008.07.145
13 JanzR, GodaY, GeppertM, et al. SV2A and SV2B function as redundant Ca2+ regulators in neurotransmitter release[J]. Neuron, 1999, 24(4): 1003-1016. doi: 10.1016/S0896-6273(00)81046-6.
doi: 10.1016/S0896-6273(00)81046-6
14 MadeoM, KovácsAD, PearceDA. The human synaptic vesicle protein, SV2A, functions as a galactose transporter in Saccharomyces cerevisiae[J]. J Biol Chem, 2014, 289(48): 33066-33071. doi:10.1074/jbc.C114.584516.
doi: 10.1074/jbc.C114.584516
15 SurgesR, Volynski KE, WalkerMC. Is levetiracetam different from other antiepileptic drugs? Levetiracetam and its cellular mechanism of action in epilepsy revisited[J]. Ther Adv Neurol Disord, 2008, 1(1): 13-24. doi:10.1177/1756285608094212.
doi: 10.1177/1756285608094212
16 TokudomeK, OkumuraT, TeradaR, et al. A missense mutation of the gene encoding synaptic vesicle glycoprotein 2A (SV2A) confers seizure susceptibility by disrupting amygdalar synaptic GABA release[J]. Front Pharmacol, 2016, 7: 210. doi:10.3389/fphar.2016.00210.
doi: 10.3389/fphar.2016.00210
17 刘鹏利, 李晓璐. 与中枢听觉处理障碍相关的下丘功能及机制探讨[J]. 国际耳鼻咽喉头颈外科杂志, 2017, 41(6): 316-321. doi:10.3760/cma.j.issn.1673-4106.2017.06.002.
doi: 10.3760/cma.j.issn.1673-4106.2017.06.002
LIUPengli, LIXiaolu. Function and mechanism of the inferior colliculus associated with central auditory processing disorder[J]. International Journal of Dermatology and Venereology, 2017, 41(6): 316-321. doi:10.3760/cma.j.issn.1673-4106.2017.06.002.
doi: 10.3760/cma.j.issn.1673-4106.2017.06.002
18 LiuJH, WangC, XiaoZJ, et al. Changes of response patterns to excitatory stimuli of different intensities: a model-based study of cochlear nucleus neurons[J]. Nan fang yi ke da xue xue bao, 2014, 34(3): 291-294. PMID: 24670436.
19 Mendoza-TorreblancaJG, Vanoye-CarloA, Phillips-FarfánBV, et al. Synaptic vesicle protein 2A: basic facts and role in synaptic function[J]. Eur J Neurosci, 2013, 38(11): 3529-3539. doi:10.1111/ejn.12360.
doi: 10.1111/ejn.12360
20 LöscherW, GillardM, SandsZA, et al. Synaptic vesicle glycoprotein 2A ligands in the treatment of epilepsy and beyond[J]. CNS Drugs, 2016, 30(11): 1055-1077. doi:10.1007/s40263-016-0384-x.
doi: 10.1007/s40263-016-0384-x
21 MuñozLJ, Carballosa-GautamMM, YanowskyK, et al. The genetic audiogenic seizure hamster from salamanca: the GASH: sal[J]. Epilepsy Behav, 2017, 71(Pt B): 181-192. doi:10.1016/j.yebeh.2016.03.002.
doi: 10.1016/j.yebeh.2016.03.002
22 StockburgerC, MianoD, BaeumlisbergerM, et al. A mitochondrial role of SV2a protein in aging and alzheimer′s disease: studies with levetiracetam[J]. J Alzheimers Dis, 2016, 50(1): 201-215. doi:10.3233/JAD-150687.
doi: 10.3233/JAD-150687
23 MattheisenM, MühleisenTW, StrohmaierJ, et al. Genetic variation at the synaptic vesicle gene SV2A is associated with schizophrenia[J]. Schizophr Res, 2012, 141(2/3): 262-265. doi:10.1016/j.schres.2012.08.027.
doi: 10.1016/j.schres.2012.08.027
24 BartholomeO, Van den AckervekenP, Sánchez GilJ, et al. Puzzling out synaptic vesicle 2 family members functions[J]. Front Mol Neurosci, 2017, 10: 148. doi:10.3389/fnmol.2017.00148.
doi: 10.3389/fnmol.2017.00148
25 de WitE, van DijkP, HanekampS, et al. Same or different: the overlap between children with auditory processing disorders and children with other developmental disorders: a systematic review[J]. Ear Hear, 2018, 39(1): 1-19. doi:10.1097/AUD.0000000000000479.
doi: 10.1097/AUD.0000000000000479
26 MuresanV, Ladescu MuresanZ. Shared molecular mechanisms in alzheimer′s disease and amyotrophic lateral sclerosis: neurofilament-dependent transport of sAPP, FUS, TDP-43 and SOD1, with endoplasmic reticulum-like tubules[J]. Neurodegener Dis, 2016, 16(1/2): 55-61. doi:10.1159/000439256.
doi: 10.1159/000439256
27 CaiZX, LiSY, MatuskeyD, et al. PET imaging of synaptic density: A new tool for investigation of neuropsychiatric diseases[J]. Neurosci Lett, 2019, 691: 44-50. doi:10. 1016/j.neulet.2018.07.038.
doi: 10. 1016/j.neulet.2018.07.038
[1] CHEN Chun, DING Jian, XIE Jin. Effects of continuous positive airway pressure therapy on inflammatory markers in obstructive sleep apnea syndrome: a meta-analysis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(4): 131-137.
[2] ZHANG Wei, ZENG Yuhan, YU Xiansong. Changes in nasal secretion of ECP, EGF and IL-6 in patients with chronic sinusitis before and after endoscopic sinus surgery and their clinical significance [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2018, 32(3): 63-67.
[3] ZHANG Huifang,YANG Hongzhen. Effects of nasal continuous positive airway pressure on glucose and lipid metabolism, hs-CRP, and [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2018, 32(2): 48-51.
[4] REN Tao, TANG Yijun, WANG Meifang, LIU Yan, QIAN Xin.. Effect of obstructive sleep apnea-hypopnea syndrome on blood glucose, serum lipid, and C-reactive protein levels. [J]. J Otolaryngol Ophthalmol Shandong Univ, 2017, 31(6): 53-57.
[5] CHENG Lei, QIAN Junjun, TIAN Huiqin. Research progresses on allergic rhinitis. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(3): 1-3.
[6] . Serum lipid changes in obstructive sleep apnea hypopnea syndrome patients underwent airway surgery. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(1): 27-30.
[7] . The aqueous levels of TNF-α and IP-10 in different kinds of glaucoma and their correlation. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2017, 31(1): 103-106.
[8] GAO Tianxi, XIA Cui, YAN Jing, HOU Jin, KANG Quanqing, WANG Zhenghui, REN Xiaoyong. Analysis of IGF-1 and IGFBP-3 level in liver tissue of rat with intermittent hypoxia. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(5): 38-41.
[9] YU Pengju, XU Fenglei, YANG Jun, CHEN Wei, JIANG Manjie. Diagnostic value of plasma 1-3-β-D glucan for fungal rhinosinusitis. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(4): 94-97.
[10] HU Wenliang, ZHENG Yanqiu, CUI Xiaobo, CUI Yanru, SUN Yuanhao. Bioinformatic screening of differentially expressed protein kinases and their inhibitors in hypopharyngeal cancer. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(3): 24-28.
[11] ZHOU Xiaoyan, LIN Peng, SUN Shiming, ZHANG Yana, LI Xiaojie, SHI Yukai. Expression and clinical significance of serum HMGB1 in sinusitis patients. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(2): 56-58.
[12] ZHANG Jian, LU Xiaoqing, WU Jian.. Differentially expressed proteins between nasal polyps and normal nasal mucosa by the iTRAQ technology. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(1): 5-8.
[13] GAO Zhenfeng, TAO Qian, CAI Xiaolan. Expression and significance of ERK5 protein in nasal polyps cells. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(1): 40-42.
[14] WANG Junying, LI Xiaofeng. Effect of deproteinized extract of calf blood gel eye on the injury of corneal endothelial cells after nasopharyngeal carcinoma radiotherapy. [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2016, 30(1): 76-79.
[15] LI Junying. Effects of rosuvastatin combined with fenofibrate therapy on vascular endothelial function retinopathy diabetic retinopathy patients [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2015, 29(5): 72-75.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
[1] LIN Bin,WANGHui-ge . Functional endoscopic sinus surgery, FESS[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2006, 20(6): 481 -487 .
[2] . [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2006, 20(1): 84 -87 .
[3] . [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2006, 20(2): 116 -118 .
[4] MA Jing, ZHONG Cui-ping . Surgical method for nasopharyngeal fibroangioma encroaching on fossa pterygopalatina: with a report of 5 cases[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2006, 20(1): 30 -32 .
[5] . [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2006, 20(1): 90 -91 .
[6] . [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 200 -203 .
[7] LIU Yan,LIU Xin-yi,WANG Jin-ping,LI Da-jian . Measurement of the posterior tympanum and its clinical significance [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 218 -221 .
[8] . [J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 227 -227 .
[9] LIN Feng,LIANG Yong,LU Yong-tian,WAN Li-xia . Classification and surgical treatment for nasal inverted papilloma[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 231 -233 .
[10] JI Xiao-bin,DENG Jia-de,ZANG Lin-quan,WANG Lei,XIE Jun . Blood histamine in guinea pigs with allergic rhinitis[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2008, 22(3): 228 -230 .
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd