JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY)

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Growth features of human embryonic lens epithelial cells cultured in vitro

ZOU Jun1, LU Yi2, CHU Ren-yuan2   

  1. 1. Department of Ophthalmology, Shanghai Sixth People′s Hospital, Shanghai Jiaotong University, Shanghai 200233, China;
    2. Department of Ophthalmology, Eye, Ear, Nose and Throat Hospital, Fudan University, Shanghai 200031, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-10-16 Published:2008-10-16
  • Contact: ZOU Jun

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Abstract: To investigate the biological characteristics of human embryonic lens epithelial cells cultured in vitro. MethodsHuman embryonic lens epithelial cells were cultured by the explanting culture methods. An inverted phase contrast microscope, electron microscope and immunocytochemistry were used to study the growth specialties of the human embryonic lens epithelial cells. ResultsHuman embryonic lens epithelial cells of primary culture in vitro grew out of the edge of tissue pieces after incubation for 48-72 hours. The primary cultured cells maintained typical morphology of the epithelial cells and reached a confluence in 10 days. Growth curves of cultured human embryonic lens epithelial cells in passage 2 showed an S-shape. After a 1-2 day latent period, the cultured cells logarithmically grew and reached a confluence in 10 days. The shape of human embryonic lens epithelial cells in passage 2 was hexagon or oval with normal ultra-structure. Positive staining of α-crystalline was shown in passage 2 cultured human embryonic lens epithelial cells by immunocytochemistry. ConclusionHuman embryonic lens epithelial cells have proliferative capacity and are suitable for related study of cataracts.

Key words: Epithelial cell, Cell culture, Lens, Human, embryo

CLC Number: 

  • R776.1
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