Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2021, Vol. 35 ›› Issue (4): 64-69.doi: 10.6040/j.issn.1673-3770.0.2021.004

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Expression and clinical significance of the KCNK5 double-pore potassium channel protein in papillary thyroid carcinoma

XUE Gang1, SUN Xinmin1, LIN Xu2, WU Jingfang2   

  1. 1. Department of Otorhinolaryngology & Head and Neck Surgery, Hebei North University, Zhangjiakou 075000, Hebei, China;
    2. Department of Histology and Embryology, Hebei North University, Zhangjiakou 075000, Hebei, China
  • Published:2021-08-05

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Abstract: Objective To detect the expression of KCNK5 in papillary thyroid carcinoma(PTC)tissues and investigate its correlation with clinicopathological characteristics and prognosis in patients with PTC. Methods Immunohistochemistry and western blotting were used to detect the expression of KCNK5 in PTC tissues and paraneoplastic normal tissues, and the relationship between KCNK5 and the clinicopathological characteristics of PTC was analyzed; moreover, the relationship between KCNK5 and the prognosis of PTC was analyzed using the KM Plotter database. Results Immunohistochemical results showed that KCNK5 was expressed in the cytosol, nuclear membrane, and cytoplasm of PTC cells, and KCNK5 was either not expressed or weakly positively expressed in paraneoplastic normal tissues. Western blotting and immunohistochemical semi-quantitative integration showed that the expression level of KCNK5 in PTC tissues was significantly higher than that in paraneoplastic normal tissues(P<0.05). KCNK5 expression was positively correlated with TNM stage and lymph node metastasis in PTC patients(both P<0.05); multivariate logistic regression analysis further indicated that KCNK5 expression was related to lymph node metastasis(P<0.05). Kaplan-Meier analysis showed that the survival rate of patients with a high KCNK5 expression was significantly lower than that of patients with a low KCNK5 expression(P<0.01). Conclusion KCNK5 is closely associated with lymph node metastasis and prognosis of PTC patients and has the potential to become an indicator of PTC prognosis.

Key words: Papillary thyroid carcinoma, Two pore domain K+ channel, KCNK5, Immunohistochemistry, Western bloting, KM Plotter

CLC Number: 

  • R736.1
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