p-mTOR、p-S6K在鼻腔鼻窦内翻性乳头状瘤中的高表达及临床价值

doi:10.6040/j.issn.1673-3770.0.2024.393

Abstract

Abstract: Objective In order to provide directions for screening molecular markers that can help identify nasal polyps and sinonasal inverted papilloma(SNIP), and to provide a theoretical basis for exploring the application of mTOR pathway inhibitors in the treatment of SNIP. Methods The differences in the expression of phosphorylated mammalian target of rapamycin(p-mTOR)and phosphorylated ribosomal protein S6 kinase(p-S6K)in SNIP compared with that of the middle turbinate mucosa were analyzed experimentally, which provided an experimental basis for verifying that the mTOR pathway mediates the pathogenesis of SNIP. Immunohistochemical staining was used to detect the expression of p-mTOR and p-S6K in the experimental group and the control group, and the differences in expression were analyzed using SPSS23.0 progressive statistics, and the results were verified by Western blotting(WB)experiments using fresh SNIP specimens and paraneoplastic specimens. Results The positive expression rates of p-mTOR and p-S6K in paraffin-embedded tissue specimens of 64 cases of SNIP were 85.94% and 81.25%, respectively, and those of p-mTOR and p-S6K in the mucosa of 32 cases of middle turbinate in the control group were 34.38% and 40.63%, respectively, and the differences were statistically significant after the statistical analysis process(χ2=26.400, P<0.01; χ2=16.103, P<0.01); WB experiments also confirmed that there were significant differences in the expression of p-mTOR and p-S6K between the SNIP experimental group and the paraneoplastic control group. Conclusion p-mTOR and p-S6K play an important role in the pathogenesis of SNIP, and the over-activation of mTOR pathway is an important mechanism to promote the pathogenesis of SNIP.

Key words: Sinonasal inverted papilloma, Immunohistochemical staining, p-mTOR, p-S6K, Protein expression

CLC Number:

R739.62

BI Heng, XIE Jia, GONG Huicheng. Clinical value of highly expressed p-mTOR and p-S6K in sinonasal inverted papilloma[J].Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(2): 25-34.

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Clinical value of highly expressed p-mTOR and p-S6K in sinonasal inverted papilloma

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