JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2016, Vol. 30 ›› Issue (5): 120-124.doi: 10.6040/j.issn.1673-3770.0.2016.106

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Comparison of allogeneic broad fascia preserved with different methods for ocular transplantation.

SONG Qi1, TANG Juan1, CAI Kejie1, PANG Kunpeng2, WEI Yan1, XU Tonghui3, ZHAI Yonghua   

  1. 1.Operating Room, Qilu Hospital of Shandong University;2.Department of Ophthalmology, Qilu Hospital of Shandong University;3. Department of Physiology, School of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Received:2016-03-14 Online:2016-10-20 Published:2016-10-20

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Abstract: Objective To compare the biological property changes of allogeneic broad fascia preserved with different methods and provide guidance for clinical application. Methods Fresh allogeneic broad fascia was randomly divided into three groups according to storage methods: groupⅠwas preserved in gradient alcohol, group Ⅱ in glycerin under deep low temperature and group Ⅲ in composite antimicrobial solution under mildly low temperature. Changes of the physical, chemical and biomechanical performance were observed in three groups.The fresh broad fascia was used as control. Results GroupⅠhad the same physical and chemical properties as fresh broad fascia after 3 months preservation, while the color and flexibility changed after being stored for 6 months. HE staining showed that the fiber density decreased gradually with the extension of storage time. Group Ⅱ and group Ⅲ had the similar physical and chemical properties as fresh broad fascia after being preserved for 6 months. There was no statistical difference of biomechanics in 3 groups after 6 months observation compared with fresh broad fascia. Conclusion All of the three methods can be used to preserve allogeneic broad fascia. However, the method of gradient alcohol is not recommended to use in the long-term or large-quantity storage of allogeneic broad fascia.

Key words: Allogeneic broad fascia, Physical and chemical properties, Biomechanical performance, Storage methods

CLC Number: 

  • R779.6
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