JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY) ›› 2018, Vol. 32 ›› Issue (3): 47-53.doi: 10.6040/j.issn.1673-3770.0.2018.068

Previous Articles     Next Articles

BRF2 in the pathogenesis of chronic rhinosinusitis with nasal polyps

GAN Bin1, ZHANG Yongju1, XU Anting2   

  1. Department of Otorhinolaryngology Head and Neck Surgery, Second Hospital of Shandong University, Jinan 250033, Shandong, China
  • Received:2018-01-27 Online:2018-05-20 Published:2018-05-20

PDF (PC)

463

Abstract: Objective To study the expression and significance of BRF2 in chronic rhinosinusitis with nasal polyps. Methods 37 surgical specimens of chronic rhinosinusitis with nasal polyps(CRSwNP)and 18 surgical specimens of simple deviation of nasal septum were collected from 2016 to 2017 in the Department of Otorhinolaryngology Head and Neck Surgery, Second Hospital of Shandong University. Immunofluorescence staining was used to detect the expression and localization of BRF2 in chronic rhinosinusitis with nasal polyps. After cultured human nasal mucosa epithelial cells were stimulated with Llipopolysaccharides(LPS)in vitro, the expression and localization of BRF2 in cells were detected by immunofluorescence staining. Western blotting and quantitative real-time PCR were used to detect the expression of BRF2 and the endoplasmic reticulum stress-related protein GRP78 in cells. Results The expression of BRF2 in chronic rhinosinusitis with nasal polyps was up-regulated in whole cells and was particularly increased in the cytoplasm fraction. In vitro, LPS stimulation can induce the up-regulation of expression of BRF2 to be up-regulated in nasal mucosal epithelial cells and increase the distribution of BRF2 in the cytoplasm, and tThe expression of endoplasmic reticulum stress-related protein GRP78 was also increased. Conclusion BRF2 may mediate endoplasmic reticulum stress, which is involved in the pathogenesis of chronic rhinosinusitis with nasal polyps.

Key words: Human nasal epithelial cells, BRF2, GRP78, Nasal polyps, Rhinosinusitis, chronic

CLC Number: 

  • R765.4+1
[1] Akdis CA, Bachert C, Cingi C, et al. Endotypes and phenotypes of chronic rhinosinusitis: a PRACTALL document of the European Academy of Allergy and Clinical Immunology and the American Academy of Allergy, Asthma & Immunology[J]. J Allergy Clin Immunol, 2013, 131(6): 1479-90.
[2] Mu(~overn)oz-Del-Castillo F, Jurado-Ramos A, Soler R, et al. Fungal sensitization in nasal polyposis[J]. J Investig Allergol Clin Immunol, 2009, 19(1): 6-12.
[3] Cabarcas S, Schramm L. RNA polymerase III transcription in cancer: the BRF2 connection[J]. Mol Cancer, 2011. 10: 47.
[4] Moreno-Moya JM, Vilella F, Simón C. MicroRNA: key gene expression regulators[J]. Fertil Steril, 2014, 101(6): 1516-1523.
[5] Lockwood WW, Chari R, Coe BP, et al. Integrative genomic analyses identify BRF2 as a novel lineage-specific oncogene in lung squamous cell carcinoma[J]. PLoS Med, 2010, 7(7): e1000315.
[6] Cabarcas S, Jacob J, Veras I, Schramm L. Differential expression of the TFIIIB subunits Brf1 and Brf2 in cancer cells[J]. BMC Mol Biol, 2008, 9: 74.
[7] Gullo F, Ceriani M, D'Aloia A, et al. Plant polyphenols and exendin-4 prevent hyperactivity and TNF-α release in LPS-treated in vitro neuron/astrocyte/microglial networks[J]. Front Neurosci, 2017, 11: 500.
[8] Shao L, Liu X, Zhu S, et al. The role of smurf1 in neuronal necroptosis after lipopolysaccharide-induced neuroinflammation[J]. Cell Mol Neurobiol, 2017. DOI:10.1007/s10571-017-0553-6.
[9] Lee S, Lee D, Jang TS, et al. Anti-inflammatory phenolic metabolites from the edible fungus phellinus baumii in LPS-stimulated RAW264.7 cells[J]. Molecules, 2017, 22(10). pii: E1583. DOI: 10.3390/molecules22101583
[10] Gaut JR, Hendershot LM. The modification and assembly of proteins in the endoplasmic reticulum[J]. Curr Opin Cell Biol, 1993, 5(4): 589-595.
[11] Hotamisligil GS. Endoplasmic reticulum stress and the inflammatory basis of metabolic disease[J]. Cell, 2010, 140(6): 900-917.
[12] Appenzeller-Herzog C, Hall MN. Bidirectional crosstalk between endoplasmic reticulum stress and mTOR signaling[J]. Trends Cell Biol, 2012, 22(5): 274-82.
[13] Ozcan L, Tabas I. Calcium signalling and ER stress in insulin resistance and atherosclerosis[J]. J Intern Med, 2016, 280(5): 457-464.
[14] Xiang C, Wang Y, Zhang H, et al. The role of endoplasmic reticulum stress in neurodegenerative disease[J]. Apoptosis, 2017, 22(1): 1-26.
[15] Zhang K, Kaufman RJ. From endoplasmic-reticulum stress to the inflammatory response[J]. Nature, 2008, 454(7203): 455-62.
[16] Malhotra JD, Miao H, Zhang K, et al. Antioxidants reduce endoplasmic reticulum stress and improve protein secretion[J]. Proc Natl Acad Sci U S A, 2008, 105(47): 18525-18530.
[17] Gouge J, Satia K, Guthertz N, et al. Redox signaling by the RNA polymerase III TFIIB-related factor Brf2[J]. Cell, 2015, 163(6): 1375-87.
[18] Guo H, Cui H, Peng X, et al. Nickel chloride(NiCl2)induces endoplasmic reticulum(ER)stress by activating UPR pathways in the kidney of broiler chickens[J]. Oncotarget, 2016, 7(14): 17508-17519.
[19] Lund VJ, Kennedy DW. Staging for rhinosinusitis[J]. Otolaryngol Head Neck Surg,1997, 117(3 Pt 2): 35-40.
[20] 鲁铭, 田辉, 李树海, 等. BRF2基因在食管鳞状细胞癌组织中的表达及其临床意义[J]. 中国肿瘤生物治疗杂志, 2012, 19(2): 175-179. LU Ming, TIAN Hui, LI Shuhai, et al. BRF2 gene expression in esophageal squamous cell carcinoma tissues and its clinical significance[J]. Chin J Cancer Biother, 2012, 19(2): 175-179.
[21] Tian Y, Lu M, Yue W, et al. TFIIB-related factor 2 is associated with poor prognosis of nonsmall cell lung cancer patients through promoting tumor epithelial-mesenchymal transition[J]. Biomed Res Int, 2014, 2014: 530786.
[22] Lu M, Tian H, Yue W, et al. TFIIB-related factor 2 over expression is a prognosis marker for early-stage non-small cell lung cancer correlated with tumor angiogenesis[J]. PLoS One, 2014, 9(2): e88032.
[1] FENG Sicong, YU Xiaolan, LOU Dan. Evaluation of the effect of Messerklinger middle turbinate plasty on chronic rhinosinusitis with nasal polyps based on the expression of Ki67 and GM-CSF in vesicular tissue [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2026, 40(3): 31-39.
[2] ZHANG Jiaqi, YUAN Ye, HONG Chen, GU Min, CHENG Lei, LU Meiping. Mendelian randomization study of gut microbiota, chronic sinusitis, and nasal polyps: Causal relationships and metabolite-mediated effects [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(5): 49-60.
[3] ZHANG Guangling, CHEN Xingxue, WU Tianyi, SUN Zhanwei, WANG Weiwei, LI Shichao,WANG Guangke. The expression and function of Tespa1 in chronic rhinosinusitis with nasal polyps [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(2): 35-42.
[4] CAO Zhengyong, LI Xiaobo. Comparison of the safety and efficacy of short-course postoperative topical glucocorticoid adjuvant therapy for eCRSwNP combined with asthma [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(2): 43-50.
[5] WANG Wenqing, ZHANG Dan, ZHU Mengdi, WANG Luyang, YANG Peipei, SUN Sisi, ZHANG Qiumin, ZHOU Hui. Changes of clinical and histopathological characteristics in recurrent chronic rhinosinusitis with nasal polyps [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(1): 46-53.
[6] WANG Aowei, SHI Wenjie. Study on correlation between traditional Chinese medicine syndrome types and TFH cell related factors in CRSwNP [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(1): 54-60.
[7] ZHANG Jie, NIMA Jizong, XU Xiaodong, ZHOU Jing, LIU Jianmin, LUO Yirui, DU Jintao, BA Luo. Effect of Crocin on Type 2 inflammation in eosinophilic chronic rhinosinusitis with nasal polyps [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(1): 61-67.
[8] SHI Zhonggang, CAI Xiaolan, LI Xuezhong, ZHANG Liqiang, FENG Xin. Maxillary cyst with inverted papilloma: a case report and literature review [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(1): 123-129.
[9] ZHANG Yunqiu, REN Xiumin, XU Ou, DONG Jinhui, WANG Jianxing. Research progress on omalizumab targeted therapy for chronic sinusitis with nasal polyps [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2025, 39(1): 136-140.
[10] CHEN Xingxue, ZHANG Guangling, WU Tianyi, WANG Weiwei, SUN Zhanwei, LI Shichao, WANG Guangke. Analysis of anti-IL-4Rα monoclonal antibody and endoscopic sinus surgery in the treatment of eosinophilic chronic rhinosinusitis with nasal polyps [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(4): 43-54.
[11] ZHANG Shihan, LIU Hongbing. Effect of matrix metalloproteinase on tissue remodeling in chronic rhinosinusitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(3): 116-123.
[12] WANG Lixue, ZENG Yi, WANG Lixin, PENG Xianbing. Clinical observation of the effect of infiltrating Biprofen Gelatin Sponge after functional endoscopic sinus surgery [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2023, 37(5): 16-25.
[13] ZHU Yu, ZHU Xinhua. Research progress on the role of TH2 cytokines in Type2 chronic rhinosinusitis with nasal polyps [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2023, 37(5): 156-161.
[14] CUI Ning, WANG Yunmeng, YANG Jingpu. Research progress on the role and regulatory mechanism of group 2 innate lymphoid cells in chronic rhinosinusitis [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2023, 37(4): 153-159.
[15] AO Tian,CHENG Lei. An endotype study of chronic rhinosinusitis with nasal polyps and precise control and treatment under the guidance [J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(3): 7-14.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
Website Copyright: Editorial Office of Journal of Otolaryngology and Ophthalmology of Shandong University
Address: No.27 Shanda South Road, Jinan, Shandong, China. Post code: 250100 Tel: +86-0531-88366912 Email: ebhxb@sdu.edu.cn Supported by Beijing Magtech Co.Ltd