标准化家猫外伤性视神经损伤动物模型的建立

doi:10.6040/j.issn.1673-3770.0.2017.433

摘要/Abstract

摘要： 目的利用统一标准的视神经夹在相同钳夹时间下对家猫视神经进行损伤实验,观察视神经部分损伤后家猫的闪光视觉诱发电位的变化,建立标准化的外伤性视神经损伤家猫动物模型。方法共有10只健康的成年家猫,每只家猫的右眼为实验眼(视神经损伤),左眼为对照眼(正常)。家猫的右眼损伤眼均以统一标准的视神经夹钳夹视神经30 s,制成家猫视神经部分损伤模型。在实验眼损伤后1 h、3 d、1周、2周、4周时分别进行实验眼和对照眼的闪光视觉诱发电位检查,记录主波P100的波峰潜时和波幅,并进行统计学分析。结果所有家猫正常对照眼的闪光视觉诱发电位的波形稳定,随时间延长均无明显变化。损伤前实验组与对照组主波P100的波峰潜时、波幅两组比较差异无统计学意义(F=1.48, P=0.19; F=1.63, P=0.15);实验组中损伤后1 h分别与损伤后3 d、1周、2周、4周各组间波峰潜时及波幅比较差异均具有统计学意义(F=7.38, P=0.008 7; F=4.38, P =0.027);损伤后3 d与损伤后1周、2周、4周各组间波峰潜时及波幅比较差异均具有统计学意义(F=6.49, P=0.019; F=3.97, P=0.036);损伤后1周与损伤后2周、4周各组间波峰潜时及波幅比较差异均具有统计学意义(F=6.02, P=0.011; F=3.37, P=0.041)。损伤后2周内 P100波峰潜时逐渐延长,波幅逐渐降低,2周以后趋于稳定,损伤后2周与损伤后4周两组间比较差异无统计学意义(F=2.24, P=0.11; F=2.01, P=0.12)。结论应用统一标准的的视神经夹可以成功建立标准化的外伤性视神经损伤家猫动物模型。闪光视觉诱发电位检查是确定标准化家猫外伤性视神经损伤动物模型建立成功与否的客观评价指标,成功的视神经损伤家猫动物模型表现为闪光视觉诱发电位主波P100的波峰潜时延长及波幅降低。

关键词: 钳夹, 模型, 闪光视觉诱发电位, 视神经损伤

Abstract: Objective To establish a standardized model of traumatic optic neuropathy in cats by using a standard clip and to simultaneously observe the changes in flash visual-evoked potential. Methods Ten adult healthy cats were enrolled in the study. The right eye was identified as the experimental eye; and the left eye, as the control eye. The optic nerves of the right eyes were clamped for 30 seconds to establish traumatic optic nerve injury models. The flash visual-evoked potential in was recorded in all experimental and control eyes at 1 hour, 3 days, 1 week, 2 weeks, and 4 weeks after injury. The peak latency and amplitude of the main wave P100 were recorded, and a statistical analysis was performed. Results The flash visual evoked potential peak latency and amplitudes of the main wave P100 were no significant differences before operation, but there were significant differences after traumatic optic neuropathy between the two groups(P< 0.05). The flash visual evoked potential peak latency and amplitudes of the main wave P100 were no significant differences before operation(F=1.48, P=0.19; F=1.63, P=0.15). In the experimental group, there were statistically significant differences between the 1 hour after injury and 3 days, 1 week, 2 weeks, and 4 weeks after the injury(F=7.38, P=0.008 7; F=4.38, P=0.027). There were statistically significant differences between 3 days after the injury and 1 week,2 weeks and 4 weeks after the injury(F=6.49, P =0.019; F=3.97, P=0.036). There were statistically significant differences between 1 week after the injury and 2 weeks and 4 weeks after the injury(F=6.02, P=0.011; F=3.37, P=0.041).The peak latency of P100 was gradually extended, and the amplitude gradually decreased in the 2 weeks after the injury. After 2 weeks, it tended to be stable. There was no statistically significant difference between the two groups after 2 weeks and 4 weeks after the injury(F=2.24, P=0.11; F=2.01, P=0.12). Conclusion The standardized cat model of traumatic optic neuropathy was successfully established by using the same clamp. The flash visual-evoked potential examination is the objective evaluation tool. The flash visual-evoked potential peak latency of the main wave P100 was significantly prolonged with a decreased amplitude, in the standardized cat model of traumatic optic neuropathy.

Key words: Optic nerve injury, Flash visual evoked potential, Clamp, Model

中图分类号:

R774.6

李俊义,李秀梅,万京明,毕京玉,刘娟,徐华林,姜彦,李娜. 标准化家猫外伤性视神经损伤动物模型的建立[J]. 山东大学耳鼻喉眼学报, 2018, 32(4): 43-47.

LI Junyi, LI Xiumei, WAN Jingming, BI Jingyu, LIU Juan, XU Hualin, JIANG Yan, LI Na. Establishment of a standardized model of traumatic optic neuropathy in cats[J]. J Otolaryngol Ophthalmol Shandong Univ, 2018, 32(4): 43-47.

参考文献

[1] 姜彦,李娜,张念凯,等.家猫外伤性视神经病的电生理及形态学观察[J].中华耳鼻咽喉头颈外科杂志,2007,42(7):520-525. JIANG Yan, LI Na, ZHANG Niankai, et al. Observation of neural electrophysiology and morphology in traumatic optic neuropathy in cat model[J]. Chin J Otorhinolaryngol Head Neck Surgery, 2007, 42(7):520-525.
[2] 丁见,杨华,张念凯.猫视神经损伤减压手术模型的建立[J].山东大学耳鼻喉眼学报,2009,23(3):75-77. DING Jian, YANG Hua, ZHANG Niankai. Model establishment of optic nerve decompression surgery in cats[J]. J Otolaryngol Ophthalmol Shandong University, 2009, 23(3):75-77.
[3] 赵玲,冯秀艳.视觉诱发电位在外伤性视神经病治疗中的临床应用[J].中华眼外伤职业眼病杂志,2012,34(1):51-53. ZHAO Ling, FENG Xiuyan. VEP clinical application in the treatment of traumatic optic neuropathy[J]. Chin J Ocular Trauma Occupational Eye Dis, 2012, 34(1):51-53.
[4] 周鑫.眼外伤后视力的视觉诱发电位评估[J].国际眼科杂志,2013,13(10):2068-2070. ZHOU Xin. Evaluation of visual evoked potentials in post-trauma eyes[J]. Inter Eye Sci, 2013, 13(10):2068-2070.
[5] Tao W, Dvoriantchikova G, Tse BC, et al. A novel mouse model of traumatic optic neuropathy using external ultrasound energy to achieve focal, indirect optic nerve injury[J]. Sci Reports, 2017, 7(1):11779.
[6] 曹霞,姜晓璇,唐永赢,等.大鼠视神经钳夹伤模型的建立及磁共振成像运用初探[J].眼科新进展,2014,34(3):205-208. CAO Xia, JIANG Xiaoxuan, TANG Yongying, et al. Establishment and pre-application of magnetic resonance imaging in rat optic nerve clamping injury model[J]. Rec Adv Ophthalmol, 2014, 34(3):205-208.
[7] 陈二涛,刘勇,冯东福,等.大鼠视神经部分损伤模型的建立[J].中华实验外科杂志,2008,25(11):1514-1516. CHEN Ertao, LIU Yong, FENG Dongfu, et al. Development of experimental partial optic nerve injury model in rats[J]. Chin J Exp Surg, 2008, 25(11):1514-1516.
[8] 李林林,温晏,宫华青.图形视觉诱发电位在猫双眼视觉研究中的作用[J].国际眼科杂志,2013,13(5):865-868. LI Linlin, WEN Yan, GONG Huaqing. The study of P-VEP on binocular vision in cats[J]. Inter Eye Sci, 2013, 13(5):865-868.
[9] 韩晓霞,许文辉,羊正祥,等.视神经损伤经颅视神经管减压与视觉诱发电位的临床研究[J].临床神经外科杂志,2014,11(6):459-461. HAN Xiaoxia, XU Wenhui, YANG Zhengxiang, et al. Clinical study on transcranial optic canal decompression and visual evoked potential for optic nerve injury[J]. J Clin Neurosurg, 2014, 11(6):459-461.
[10] 贾云峰,胡国良,李建.颅脑损伤伴视神经损伤86例临床分析[J].临床神经外科杂志,2013,10(2):90-91. JIA Yunfeng, HU Guoliang, LI Jian. Clinical analysis of 86 patients with head trauma and traumatic optic neuropathy[J]. J Clin Neurosurg, 2013, 10(2):90-91.
[11] 魏洪润,张哲成,田丽,等.视觉诱发电位对糖尿病视网膜病变的早期诊断价值[J].山东医药,2014,54(13):45-46.
[12] Luo Yi-ting. Diagnostic value of VEP applied in patients with optic nerve contusion[J].Mod Diagn Treat, 2013, 24(5):963-964.
[13] 赵秋岭.视觉诱发电位对外伤性视神经损伤诊断的应用价值[J].中国伤残医学,2014,22(9):206-207.
[14] You Y, K1istomar A, Thie J, et al. Axonal loss in a rat model of optic neuritisis closely correlated with visual evoked potential amplitudes using electroencephalogram-based sealing[J]. Invest Ophthalmol Vis Sci, 2012, 53(7):3662-3663.
[15] Heravian J, Ehyaei A, Shoeihi V, et al. Pattern Visual Evoked potentials patients with type II diabetes mellitus[J]. Ophthamic Vis Res, 2012, 7(3):225-230.
[16] Xue F, Wu K, Wang T, et al. Morphological and functional changes of the optic nerve following traumatic optic nerve injuries in rabbits[J]. Biomed Rep, 2016, 4(2):188-192.

相关文章 15

[1]	李凯,罗丹. 润目灵方激活LC3-ATG5自噬通路抑制炎症因子表达改善干眼大鼠眼表损伤的机制[J]. 山东大学耳鼻喉眼学报, 2026, 40(3): 92-101.
[2]	刘佳珲,田汝宪,李玉梅,宋西成. 构建铜死亡相关lncRNA模型评估头颈鳞状细胞癌预后[J]. 山东大学耳鼻喉眼学报, 2026, 40(2): 49-64.
[3]	章娜娜,黄冠江,卢标清. 联合中医证型分析喉癌变的影响因素及构建预测模型[J]. 山东大学耳鼻喉眼学报, 2026, 40(1): 29-37.
[4]	秦娜娜,李玉芬,孙雨浩,魏健,李钦. 基于TGF-β1/Smad信号通路探讨白细胞介素13受体α2对变应性鼻炎大鼠鼻黏膜组织重塑的影响[J]. 山东大学耳鼻喉眼学报, 2025, 39(6): 71-77.
[5]	刘丹凤,潘庆春,李代波,封雪飞,任健. 1990—2021年中国归因于过量饮酒的喉癌疾病负担分析及未来趋势预测[J]. 山东大学耳鼻喉眼学报, 2025, 39(5): 61-69.
[6]	姚雪,陆小凤,张梦芮,胡馨雅,赵嘉洛,赖思思,李玄,刘子潇,沈超凡,范梓欣,张寅升,张国明. 基于YOLOv8模型辅助诊断斜肌功能异常[J]. 山东大学耳鼻喉眼学报, 2025, 39(5): 76-82.
[7]	吕勇,冯云. 双感官障碍人群心血管疾病风险的预测模型:基于CHARLS的分析[J]. 山东大学耳鼻喉眼学报, 2025, 39(3): 122-128.
[8]	李晓宇,王彬晨,徐大朋,王艳华,杜平功,张庆泉. 三维建模配准分析经口与经鼻开窗治疗上颌骨囊肿三维形态变化[J]. 山东大学耳鼻喉眼学报, 2025, 39(2): 72-78.
[9]	李培培,卢彦青,侯楠. 机器学习预测模型在突发性聋中的临床应用研究[J]. 山东大学耳鼻喉眼学报, 2025, 39(2): 145-151.
[10]	李志成,闫亚,戴梁平,应俊杰, 王仁忠. 嗅觉障碍小鼠和大鼠模型的研究进展[J]. 山东大学耳鼻喉眼学报, 2025, 39(2): 158-167.
[11]	毕赵静,李元彬. 睑板腺功能障碍实验模型及应用现状[J]. 山东大学耳鼻喉眼学报, 2024, 38(4): 159-165.
[12]	王靖淞,余灿,张西,邓启成,谢卓良,赵锐,温蓓,刘海. KRT4在喉癌及癌旁正常黏膜组织中的表达及临床意义[J]. 山东大学耳鼻喉眼学报, 2024, 38(3): 12-17.
[13]	代红磊,王秋阳,马文学,官兵,齐静静. 基于Joinpoint回归及年龄-时期-队列模型分析鼻咽癌患者的死亡率发展趋势[J]. 山东大学耳鼻喉眼学报, 2024, 38(1): 27-31.
[14]	乌日柴夫,苏日古格,孟永梅. 卵清蛋白诱导的过敏性鼻炎豚鼠模型的建立与评价及蒙医舌尿诊的初步分析[J]. 山东大学耳鼻喉眼学报, 2024, 38(1): 32-38.
[15]	张一彤,李青香,石争浩,尚磊,袁钰淇,曹子讷,麻莉娜,刘海琴,任晓勇,施叶雯. 阻塞性睡眠呼吸暂停儿童睡眠结构研究及睡眠结构判读模型建立[J]. 山东大学耳鼻喉眼学报, 2023, 37(6): 126-132.

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed