Journal of Otolaryngology and Ophthalmology of Shandong University ›› 2025, Vol. 39 ›› Issue (1): 61-67.doi: 10.6040/j.issn.1673-3770.0.2024.027

• Original Article • Previous Articles     Next Articles

Effect of Crocin on Type 2 inflammation in eosinophilic chronic rhinosinusitis with nasal polyps

ZHANG Jie1,2, NIMA Jizong2, XU Xiaodong3, ZHOU Jing4, LIU Jianmin4, LUO Yirui1,2, DU Jintao4, BA Luo1,2   

  1. 1. Medical College of Tibet University, Lasa 850000, Xizang, China 2. Department of Otorhinolaryngology, People's Hospital of Tibet Autonomous Region, Lasa 850000, Xizang, China3. Department of Otolaryngology, Shannan City People's Hospital, Shannan 856000, Xizang, China4. Department of Otorhinolaryngology & Head and Neck Surgery, West China Hospital, Sichuan University, Chengdu 610041, Sichuan,  China
  • Published:2025-01-17

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Abstract: Objective To study the effect of crocin on Eosinophilic Chronic Rhinosinusitis with Nasal Polyps, the regulatory role and mechanism of Peripheral blood mononuclear cell(PBMC)type 2 inflammation in patients with Eos CRSwNP, providing a new target or pathway for precise treatment of nasal polyps. Methods Chronic rhinosinusitis with nasal polyps(CRSwNP)patients were collected for pathological diagnosis. Eosinophilic Chronic Rhinosinusitis with Nasal Polyps(Eos CRSwNP)and Non-eosinophilic Chronic Rhinosinusitis with Nasal Polyps(Non-Eos CRSwNP)were classified according to the inflammatory cell types. PBMC in peripheral blood of Eos CRSwNP patients was extracted by density gradient centrifugation. PBMC was stimulated with different concentrations of IL-33, and transcription factors GATA3, T-bet, and cell receptor CRTH2 were detected by qPCR. The expression of inflammatory cytokines INF-γ, IL-5, and IL-13 was analyzed by qPCR and ELISA. Then different concentrations of crocin were added for intervention, and GATA3, T-bet, and CRTH2 were detected by Western blotting and qPCR. GraphPad Prism software was used for statistical analysis to compare whether there were significant differences in index changes before and after IL-33 stimulation and crocin intervention. Results A total of 16 cases were pathologically confirmed as Eos CRSwNP. After stimulating PBMC with different concentrations of IL-33, qPCR confirmed that the expression of T-bet, GATA3, and CRTH2 was significantly increased(P values were 0.004, 0.001, and 0.026, respectively). qPCR and ELISA further revealed upregulation of cytokines INF-γ, IL-5, and IL-13(P values of qPCR were 0.001, 0.001, and 0.003, respectively; P values of ELISA were 0.035, 0.006, and 0.001, respectively), with the most significant activation of PBMC inflammatory cells observed at an IL-33 concentration of 20 ng/mL. After intervention with crocin, Western blotting and qPCR showed a downward trend in the expression of T-bet, GATA3, and CRTH2, with the most significant effect observed at a concentration of 10 μg/mL(P values of WB were 0.027, 0.001, and 0.002, respectively; P values of qPCR were 0.019, 0.001, and 0.001, respectively). Conclusion Crocin can significantly inhibit type 2 inflammation in IL-33-activated CRSwNP patients and has potential value in the development of new therapeutic drugs for CRSwNP.

Key words: Chronic rhinosinusitis, Nasal polyps, Eosinophils, Peripheral blood mononuclear cells, Crocin, Type 2 inflammatory effect

CLC Number: 

  • R765.41
[1] 张罗. 生物制剂治疗慢性鼻窦炎鼻息肉的现状和展望[J]. 临床耳鼻咽喉头颈外科杂志, 2023, 37(11): 853-855. doi:10.13201/j.issn.2096-7993.2023.11.001
[2] 徐小东.藏红花活性成分鼻喷剂治疗中重度变应性鼻炎的临床疗效观察[D].西藏大学,2023
[3] Aslani MR, Jafari Z, Rahbarghazi R, et al. Effects of crocin on T-bet/GATA-3 ratio, and miR-146a and miR-106a expression levels in lung tissue of ovalbumin-sensitized mice[J]. Iran J Basic Med Sci, 2022, 25(10): 1267-1274. doi:10.22038/IJBMS.2022.65622.14433
[4] 中华耳鼻咽喉头颈外科杂志编辑委员会鼻科组, 中华医学会耳鼻咽喉头颈外科学分会鼻科学组. 中国慢性鼻窦炎诊断和治疗指南(2018)[J]. 中华耳鼻咽喉头颈外科杂志, 2019, 54(2): 81-100. doi:10.3760/cma.j.issn.1673-0860.2019.02.001
[5] Fokkens WJ, Lund VJ, Hopkins C, et al. European position paper on rhinosinusitis and nasal polyps 2020[J]. Rhinology, 2020, 58(29): 1-464. doi:10.4193/Rhin20.600
[6] Bai JQ, Huang JH, Price CPE, et al. Prognostic factors for polyp recurrence in chronic rhinosinusitis with nasal polyps[J]. J Allergy Clin Immunol, 2022, 150(2): 352-361.e7. doi:10.1016/j.jaci.2022.02.029
[7] 朱玉,朱新华. TH2细胞因子在2型慢性鼻窦炎伴鼻息肉中的作用机制研究进展[J]. 山东大学耳鼻喉眼学报, 2023, 37(5): 156-161. doi:10.6040/j.issn.1673-3770.0.2022.258 ZHU Yu, ZHU Xinhua. Research progress on the role of TH2 cytokines in Type2 chronic rhinosinusitis with nasal polyps[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2023, 37(5): 156-161. doi:10.6040/j.issn.1673-3770.0.2022.258
[8] Lou HF, Meng YF, Piao YS, et al. Predictive significance of tissue eosinophilia for nasal polyp recurrence in the Chinese population[J]. Am J Rhinol Allergy, 2015, 29(5): 350-356. doi:10.2500/ajra.2015.29.4231
[9] Carsuzaa F, Béquignon é, Dufour X, et al. Cytokine Signature and Involvement in Chronic Rhinosinusitis with Nasal Polyps[J]. Int J Mol Sci, 2021, 23(1): 417. doi:10.3390/ijms23010417
[10] Kidoguchi M, Imoto Y, Noguchi E, et al. Middle meatus microbiome in patients with eosinophilic chronic rhinosinusitis in a Japanese population[J]. J Allergy Clin Immunol, 2023, 152(6): 1669-1676.doi:10.1016/j.jaci.2023.06.029
[11] Du JT, Ba L, Li B, et al. Distinct expression of NK2 homeobox 1(NKX2-1)and goblet cell hyperplasia in nasal polyps with different endotypes[J]. Int Forum Allergy Rhinol, 2017, 7(7): 690-698. doi:10.1002/alr.21932
[12] 敖天. 慢性鼻窦炎伴鼻息肉的内型研究及其指导下的精准控制与治疗[J].山东大学耳鼻喉眼学报,2022, 36(3): 7-14. doi:10.6040/j.issn.1673-3770.1.2022.005 AO Tian. An endotype study of chronic rhinosinusitis with nasal polyps and precise control and treatment under the guidance[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2022, 36(3): 7-14. doi:10.6040/j.issn.1673-3770.1.2022.005
[13] Zhou YL, Xu ZD, Liu ZQ. Role of IL-33-ST2 pathway in regulating inflammation: current evidence and future perspectives[J]. J Transl Med, 2023, 21(1): 902. doi:10.1186/s12967-023-04782-4
[14] Olguín-Martínez E, Muñoz-Paleta O, Ruiz-Medina BE, et al. IL-33 and the PKA Pathway Regulate ILC2 Populations Expressing IL-9 and ST2[J]. Front Immunol, 2022, 13:787713. doi:10.3389/fimmu.2022.787713
[15] Shakerian L, Kolahdooz H, Garousi M, et al. IL-33/ST2 axis in autoimmune disease[J]. Cytokine, 2022, 158: 156015. doi:10.1016/j.cyto.2022.156015
[16] 杨卜嘉, 于传飞, 侯书婷, 等. IL-33/ST2信号通路及相关药物研究进展[J].中国药事,2024,38(1):96-104. doi:10.16153/j.1002-7777.2024.01.013 YANG Bojia, YU Chuanfei, HOU Shuting, et al. Research advances in IL-33/ST2 signaling pathway and related drugs[J]. Chinese Pharmaceutical Affairs, 2024, 38(1): 96-104. doi:10.16153/j.1002-7777.2024.01.013
[17] Choi MR, Sosman JA, Zhang B. The janus face of IL-33 signaling in tumor development and immune escape[J]. Cancers(Basel), 2021, 13(13): 3281. doi: 10.3390/cancers13133281
[18] Cayrol C, Girard JP. Interleukin-33(IL-33): a critical review of its biology and the mechanisms involved in its release as a potent extracellular cytokine[J]. Cytokine, 2022, 156: 155891. doi:10.1016/j.cyto.2022.155891
[19] Jin J, Fan YJ, Nguyen TV, et al. Fallopia japonica root extract ameliorates ovalbumin-induced airway inflammation in a CARAS mouse model by modulating the IL-33/TSLP/NF-κB signaling pathway[J]. Int J Mol Sci, 2023, 24(15): 12514. doi: 10.3390/ijms241512514
[20] Anaeigoudari F, Anaeigoudari A, Kheirkhah-Vakilabad A. A review of therapeutic impacts of saffron(Crocus sativus L.)and its constituents[J]. Physiol Rep, 2023, 11(15): 15785. doi:10.14814/phy2.15785
[21] Bastani S, Vahedian V, Rashidi M, et al. An evaluation on potential anti-oxidant and anti-inflammatory effects of Crocin[J]. Biomed Pharmacother, 2022, 153: 113297. doi:10.1016/j.biopha.2022.113297
[17] 浦诗磊, 徐宏鸣, 李晓艳. 儿童鼻骨骨折的临床特征分析[J].中国眼耳鼻喉科杂志, 2023, 23(1):60-63, 72. doi: 10.14166/j.issn.1671-2420.2023.01.012 PU Shilei, XU Hongming, LI Xiaoyan. Clinical characteristics of nasal bone fracture in children[J]. Chinese Journal of Ophthalmology and Otorhinolaryngology, 2023, 23(1):60-63, 72. doi: 10.14166/j.issn.1671-2420.2023.01.012
[18] 危高生, 周颖, 李育广, 等. 高频超声联合鼻内镜引导下外伤性鼻骨骨折伴鼻中隔骨折同期手术治疗的疗效分析[J]. 中国实用医药, 2019, 14(33)26-28. doi: 10.14163/j.cnki.11-5547/r.2019.33.011 WEI Gaosheng, ZHOU Ying, LI Yuguang, et al. Therapeutic effect of high-frequency ultrasonography combined with simultaneous surgery on endoscopic guided traumatic nasal bone fracture with nasal septal fracture[J]. China Practical Medical, 2019, 14(33)26-28. doi: 10.14163/j.cnki.11-5547/r.2019.33.011
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