调节性T细胞与变应性鼻炎特异性脱敏治疗效果的关系

doi:10.6040/j.issn.1673-3770.0.2014.189

摘要/Abstract

摘要： 目的探讨外周血Treg细胞和IL-10水平在变应性鼻炎患者不同疗程特异性脱敏治疗后的差异以及能否预测特异性脱敏治疗的疗效。方法收集变应性鼻炎(AR)患者60例，随机分配至不同疗程治疗组(12个月治疗组和24个月治疗组)。治疗前后采集外周血，用流式细胞仪检测外周血中Treg细胞(Foxp3⁺CD4⁺CD25⁺细胞)占外周血单核细胞的比例和酶联免疫分析试剂盒检测IL-10水平。结果 AR患者外周血Treg细胞百分比和IL-10水平明显低于正常对照组； AR患者在特异性脱敏治疗后,外周血Treg细胞百分比和IL-10水平较治疗前明显提高。相关分析提示外周血IL-10水平与Treg细胞百分比呈正相关性。24个月疗程脱敏治疗组比12个月疗程治疗组有效率更高，且24个月疗程患者治疗后其外周血Treg细胞百分比和IL-10水平明显高于12个月疗程组。脱敏治疗有效人群治疗后的IL-10水平明显高于治疗前，而治疗无效人群治疗前后的IL-10水平无统计学差异。结论外周血Treg细胞水平在一定程度上可以预测特异性脱敏治疗的疗效；24个月疗程脱敏治疗效果优于12个月疗程脱敏治疗。

关键词: 特异性脱敏治疗, 变应性鼻炎, 调节性T细胞

Abstract: Objective To explore the expression difference of the levels of Treg cells and IL-10 of patients with allergic rhinitis (AR) in serum in different courses of special immunotherapy treatment (SIT) and to explore whether the expressions can forecast the efficacy of SIT. Methods From January 2011, 30 patients with AR were randomly collected and distributed into the 12 months SIT group and the 24 months SIT group. The peripheral blood was taken before and after the SIT, and the expressions of Treg cells were measured by FCM and IL-10 were calculated by ELISA. Results The percentage of Treg cells and the level of IL-10 in the AR groups were significantly decreased compared with those of the control group; After SIT, the percentage of Treg cells and the level of IL-10 in serum were significantly increased than those before SIT. Correlation analysis showed IL-10 was positively related with Treg cells. The clinical efficacy of the 24 months SIT group were higher than that of the 12 months SIT group, and furthermore the percentage of Treg cells and the level of IL-10 in the 24 month SIT group were significantly higher than those in the 12 month SIT group. In effective group, IL-10 level increased significantly after the SIT treatment, while the level of IL-10 did not significantly change after the treatment in non-effective group. Conclusion The percentage of Treg cells in peripheral blood of patients with AR may be useful in predicting the efficacy of the SIT treatment. A 24-month course SIT treatment is better than a 12-month course SIT treatment.

Key words: Allergic rhinitis, Special immunotherapy treatment, T regulatory cell

中图分类号:

R765.21

于阅尽, 谭骏, 曹云虹, 张慧, 贾秀华, 叶青. 调节性T细胞与变应性鼻炎特异性脱敏治疗效果的关系[J]. 山东大学耳鼻喉眼学报, 2014, 28(5): 38-41.

YU Yue-jin, TAN Jun, CAO Yun-hong, ZHANG Hui, JIA Xiu-hua, YE Qing. Relationship between Treg cells in peripheral blood of patients with allergic rhinitis and special immunotherapy efficacy[J]. JOURNAL OF SHANDONG UNIVERSITY (OTOLARYNGOLOGY AND OPHTHALMOLOGY), 2014, 28(5): 38-41.

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