山东大学耳鼻喉眼学报 ›› 2016, Vol. 30 ›› Issue (3): 68-72.doi: 10.6040/j.issn.1673-3770.0.2015.456

• 论著 • 上一篇    下一篇

cAxin2参与鸡胚内耳早期发育

张小兵1,戴方平2,侯赟1,Brand-Saberi B2   

  1. 1.兰州大学第一医院耳鼻咽喉头颈外科, 甘肃 兰州 730000;
    2. Department of Anatomy and Molecular Embryology, Institute of Anatomy, Ruhr University Bochum, 44780 Bochum, Germany
  • 收稿日期:2015-10-30 出版日期:2016-06-16 发布日期:2016-06-16
  • 通讯作者: 张小兵. E-mail:790736924@qq.com; Beate Brand-Saberi. E-mail:beate.brand-saberi@rub.de E-mail:ytchenxm@163.co
  • 基金资助:
    甘肃省卫生厅行业科技计划项目(GSWST2011-06)

Participation of cAxin2 in the development of inner ear of chick embryo.

ZHANG Xiaobing1, DAI Fangping2, HOU Yun1, Brand-Saberi B   

  1. 1. Department of Otolaryngology &Head and Neck Surgery, First Hospital of Lanzhou University, Lanzhou 730000, Gansu, China;2. Department of Anatomy and Molecular Embryology, Institute of Anatomy, Ruhr University Bochum, 44780 Bochum, Germany
  • Received:2015-10-30 Online:2016-06-16 Published:2016-06-16

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摘要： 目的 观察Wnt家族负调节因子cAxin2在鸡胚内耳早期发育中的表达方式。 方法 制备cAxin2的探针,并用地高辛RNA标记盒(Roche)标记。将受精白来亨鸡蛋孵化,按Hamburger和Humilton(1951)标准分期,宰杀HH11-28各期鸡胚并固定,全胚胎原位杂交。挑选合适鸡胚并用震动切片机切片。 结果 鸡的等位基因cAxin2的表达始于HH-14+期发育内耳的耳杯中,并在内耳的形成及进一步耳囊的分化过程中显示了一个动态的表达方式。 结论 cAxin2参与鸡胚内耳发育。

关键词: cAxin2, 鸡, 内耳发育

Abstract: Objective To observe the expression of the Wnt families negative regulator cAxin2 in the development of inner ear of chick embryo. Methods The fertilized white leghorn eggs were hatched and chick embryos were butchered in HH11-28 stages according to the Hamburger and Humilton(1951)staging criteria. The embroys were fixed and hybridized in situ and sectioned. Results Chick homolog gene, cAxin2, expressed in the otic cup beginning at stage HH14+ and exhibited a dynamic expression pattern during formation and further differentiation of the otic vesicle. Conclusion cAxin2 participate in inner ear development of Chick embryo.

Key words: Chick, cAxin2, Inner ear development

中图分类号: 

  • R764
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