山东大学耳鼻喉眼学报 ›› 2017, Vol. 31 ›› Issue (4): 64-67.doi: 10.6040/j.issn.1673-3770.0.2016.494

• 论著 • 上一篇    下一篇

RASSF2A基因甲基化在喉癌组织中的表达及意义

张明德1,张祖平2,于学民3,魏燕红1,袁英3   

  1. 1.聊城市东昌府人民医院耳鼻喉科, 山东 聊城 252000;
    2.聊城市人民医院耳鼻喉科, 山东 聊城 252000;
    3.山东大学齐鲁医院耳鼻咽喉头颈外科, 山东 青岛 266035
  • 收稿日期:2016-11-23 出版日期:2017-08-16 发布日期:2017-08-16
  • 通讯作者: 袁英. E-mail:yuanying1973@163.com

RASSF2A methylation in laryngeal squamous cell carcinoma.

ZHANG Mingde1, ZHANG Zuping2, YU Xuemin3, WEI Yanhong1, YUAN Ying3   

  1. 1. Department of Otolaryngology, Liaocheng Dongchangfu Peoples Hospital, Liaocheng 252000, Shangdong, China;2. Department of Otolaryngology, Liaocheng Peoples Hospital, Liaocheng 252000, Shangdong, China;3. Department of Otolaryngology &Head and Neck Surgery, Qilu Hospital of Shandong University, Qingdao 266035, Shandong, China
  • Received:2016-11-23 Online:2017-08-16 Published:2017-08-16

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摘要： 目的 探讨抑癌基因RASSF2A表达水平及其基因异常甲基化与喉癌的关系及意义。 方法 采用RT-PCR及甲基化特异性PCR(MSP)检测50例喉癌组织(实验组)和10例癌旁正常黏膜组织(对照组)中RASSF2A基因mRNA的表达水平及其启动子甲基化状态。 结果 喉癌组织中有54%(27/50)存在RASSF2A基因启动子,而对照组正常黏膜组织中未发现RASSF2A基因甲基化现象,差异有统计学意义(χ2=9.818, P=0.002)。RASSF2A甲基化水平程度与喉癌患者临床病理特征无明显相关性。喉癌组织中RASSF2A基因mRNA的表达水平明显低于正常黏膜组织(χ2=9.818, P=0.002)。RASSF2A基因甲基化的喉癌组织中其mRNA的表达减少,反之亦然。 结论 喉癌组织中RASSF2A基因CpG岛甲基化与其基因表达缺失有关,可能参与了喉癌的发生发展过程。

关键词: 抑癌基因RASSF2A, 喉肿瘤, 甲基化

Abstract: Objective To evaluate the effects of methylation on RASSF2A expression during the development of laryngeal squamous cell carcinoma(LSCC). Methods Methylation-specific PCR was used to measure RASSF2A methylation and mRNA levels in tissues from 50 LSCCs(LSCC group)and 10 normal laryngeal tissues(control group). Results Hypermethylation was detected in the promoter region of RASSF2A in 54%(27/50)of the laryngeal cancer tissue samples but was not detected in control group tissues. The difference in hypermethylation was statistically significant(χ2=9.818, P=0.002). No significant correlations were found between the methylation levels of RASSF2A and the clinicopathological features of the patients with laryngeal cancer. The levels of RASSF2A mRNA in the LSCC group were significantly lower than those in normal mucosal tissues(χ2=9.818, P=0.002), because of methylation in RASSF2A in the laryngeal cancer tissue samples. Conclusion Epigenetic inactivation of RASSF2A through aberrant promoter methylation may play an important role during oncogenesis and the progression of LSCC.

Key words: Methylation, RASSF2A, Laryngeal squamous cell carcinoma

中图分类号: 

  • R739.65
[1] Karatzanis AD, Psychogios G, Waldfahrer F, et al. Management of locally advanced laryngeal cancer[J]. J Otolaryngol Head Neck Surg, 2014, 43(1):4.
[2] Gurbuz MK, Acikalin M, Tasar S, et al. Clinical effectiveness of thyroidectomy on the management of locally advanced laryngeal cancer[J]. Auris Nasus Larynx, 2014, 41(1):69-75.
[3] 李万举,孙敬武,别远志. Ras相关区域家族1A基因在喉癌及喉角化症组织中的表达及临床意义[J]. 听力学及言语疾病杂志,2014,22(6):616-619. LI Wanju, SUN Jingwu, BIE Yuanzhi. ImmunohistochemicaI expression and cIinicaI impIication of RASSF1A gene in laryngeaI squamous ceII carcinoma and keratosis of the larynx[J]. Journal of Audiology and Speech Pathology, 2014, 22(6):616-619.
[4] 许承弼,腾博,李长青,等. 喉鳞状细胞癌中RASSF1A基因启动子区甲基化及蛋白表达[J].吉林大学学报(医学版),2006,32(2):326-329. XU Chengbi, TENG Bo, LI Changqing, et al. Promoter methylation of RASSF1A gene in laryngeal squamous cell carcinoma and protein expression[J]. J Jilin Univ(Med Edition), 2006, 32(2):326-329.
[5] Wu Y, Zhang X, Lin L, et al. Aberrant methylation of RASSF2A in tumors and plasma of patients with epithelial ovarian cancer[J]. Asian Pac J Cancer Prev, 2014, 15(3):1171-1176.
[6] Zhang X, Ma Y, Wu Y, et al. Aberrant promoter methylation and silencing of RASSF2A gene in cervical cancer[J]. J Obstet Gynaecol Res, 2014, 40(5):1375-1381.
[7] 任芳,王丹波. RASSF2基因甲基化与卵巢透明细胞癌的相关性[J]. 中国医科大学学报,2014,43(11):969-972. REN Fang, WANG Danbo. Investigation on the correlation between hypermethylation of RASSF2 and clear cell ovarian carcinoma[J]. Journal of China Medical University, 2014, 43(11):969-972.
[8] Costello JF, Plass C. Methylation matters[J]. J Med Gent, 2001, 38(5):285-303.
[9] Herman JG, Baylin SB. Gene silencing in cancer in association with promoter hypermethylation[J]. N Engl J Med, 2003, 349(21):2042-2054.
[10] Akino K, Toyota M, Suzuki H, et al. The Ras effector RASSF2 is a novel tumor-suppressor gene in human colorectal cancer[J]. Gastroenterology, 2005, 129(1):156-169.
[11] Hesson LB, Wilson R, Morton D, et al. CpG island promoter hypermethylation of a novel Ras effector gene RASSF2A is an early event in colon carcinogenesis and correlates inversely with K-ras mutuation[J]. Oncogene, 2005, 24(24):3987-3994.
[12] Park HW, Kang HC, Kim IJ, et al. Correlation between hypermethylation of the RASSF2A promoter and K-ras/BRAF mutations in microsatellite-stable colorectal cancers[J]. Int J Cancer, 2007,120(1):7-12.
[13] Liao X, Siu MK, Chan KY, et al. Hypermethylation of RAS effector related genes and DNA methyltransferase 1 expression in endometrial carciongenesis[J]. Int J Cancer, 2008, 123(2):296-302.
[14] Zhang Z, Sun D, Van do N, et al. Inactivation of RASSF2A by promoter methylation correlates with lymph node metastasis in nasopharyngeal carcinoma[J]. Int J Cancer, 2007, 120(1):32-38.
[15] Zhao L, Cui Q, Lu Z, et al. Aberrant methylation of RASSF2A in human pancreatic ductal adenocarcinoma and its relation to clinicopathologic features[J]. Pancreas, 2012, 41(2):206-211.
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