标准化家猫外伤性视神经损伤动物模型的建立

doi:10.6040/j.issn.1673-3770.0.2017.433

摘要/Abstract

摘要： 目的利用统一标准的视神经夹在相同钳夹时间下对家猫视神经进行损伤实验,观察视神经部分损伤后家猫的闪光视觉诱发电位的变化,建立标准化的外伤性视神经损伤家猫动物模型。方法共有10只健康的成年家猫,每只家猫的右眼为实验眼(视神经损伤),左眼为对照眼(正常)。家猫的右眼损伤眼均以统一标准的视神经夹钳夹视神经30 s,制成家猫视神经部分损伤模型。在实验眼损伤后1 h、3 d、1周、2周、4周时分别进行实验眼和对照眼的闪光视觉诱发电位检查,记录主波P100的波峰潜时和波幅,并进行统计学分析。结果所有家猫正常对照眼的闪光视觉诱发电位的波形稳定,随时间延长均无明显变化。损伤前实验组与对照组主波P100的波峰潜时、波幅两组比较差异无统计学意义(F=1.48, P=0.19; F=1.63, P=0.15);实验组中损伤后1 h分别与损伤后3 d、1周、2周、4周各组间波峰潜时及波幅比较差异均具有统计学意义(F=7.38, P=0.008 7; F=4.38, P =0.027);损伤后3 d与损伤后1周、2周、4周各组间波峰潜时及波幅比较差异均具有统计学意义(F=6.49, P=0.019; F=3.97, P=0.036);损伤后1周与损伤后2周、4周各组间波峰潜时及波幅比较差异均具有统计学意义(F=6.02, P=0.011; F=3.37, P=0.041)。损伤后2周内 P100波峰潜时逐渐延长,波幅逐渐降低,2周以后趋于稳定,损伤后2周与损伤后4周两组间比较差异无统计学意义(F=2.24, P=0.11; F=2.01, P=0.12)。结论应用统一标准的的视神经夹可以成功建立标准化的外伤性视神经损伤家猫动物模型。闪光视觉诱发电位检查是确定标准化家猫外伤性视神经损伤动物模型建立成功与否的客观评价指标,成功的视神经损伤家猫动物模型表现为闪光视觉诱发电位主波P100的波峰潜时延长及波幅降低。

关键词: 钳夹, 模型, 闪光视觉诱发电位, 视神经损伤

Abstract: Objective To establish a standardized model of traumatic optic neuropathy in cats by using a standard clip and to simultaneously observe the changes in flash visual-evoked potential. Methods Ten adult healthy cats were enrolled in the study. The right eye was identified as the experimental eye; and the left eye, as the control eye. The optic nerves of the right eyes were clamped for 30 seconds to establish traumatic optic nerve injury models. The flash visual-evoked potential in was recorded in all experimental and control eyes at 1 hour, 3 days, 1 week, 2 weeks, and 4 weeks after injury. The peak latency and amplitude of the main wave P100 were recorded, and a statistical analysis was performed. Results The flash visual evoked potential peak latency and amplitudes of the main wave P100 were no significant differences before operation, but there were significant differences after traumatic optic neuropathy between the two groups(P< 0.05). The flash visual evoked potential peak latency and amplitudes of the main wave P100 were no significant differences before operation(F=1.48, P=0.19; F=1.63, P=0.15). In the experimental group, there were statistically significant differences between the 1 hour after injury and 3 days, 1 week, 2 weeks, and 4 weeks after the injury(F=7.38, P=0.008 7; F=4.38, P=0.027). There were statistically significant differences between 3 days after the injury and 1 week,2 weeks and 4 weeks after the injury(F=6.49, P =0.019; F=3.97, P=0.036). There were statistically significant differences between 1 week after the injury and 2 weeks and 4 weeks after the injury(F=6.02, P=0.011; F=3.37, P=0.041).The peak latency of P100 was gradually extended, and the amplitude gradually decreased in the 2 weeks after the injury. After 2 weeks, it tended to be stable. There was no statistically significant difference between the two groups after 2 weeks and 4 weeks after the injury(F=2.24, P=0.11; F=2.01, P=0.12). Conclusion The standardized cat model of traumatic optic neuropathy was successfully established by using the same clamp. The flash visual-evoked potential examination is the objective evaluation tool. The flash visual-evoked potential peak latency of the main wave P100 was significantly prolonged with a decreased amplitude, in the standardized cat model of traumatic optic neuropathy.

Key words: Optic nerve injury, Flash visual evoked potential, Clamp, Model

中图分类号:

R774.6

李俊义,李秀梅,万京明,毕京玉,刘娟,徐华林,姜彦,李娜. 标准化家猫外伤性视神经损伤动物模型的建立[J]. 山东大学耳鼻喉眼学报, 2018, 32(4): 43-47.

LI Junyi, LI Xiumei, WAN Jingming, BI Jingyu, LIU Juan, XU Hualin, JIANG Yan, LI Na. Establishment of a standardized model of traumatic optic neuropathy in cats[J]. J Otolaryngol Ophthalmol Shandong Univ, 2018, 32(4): 43-47.

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