盐酸氯米帕明保护听觉毛细胞免受新霉素诱导的损伤

doi:10.6040/j.issn.1673-3770.0.2023.200

摘要/Abstract

摘要： 目的筛选出能有效抑制新霉素导致的毛细胞系(HEI-OC1)细胞死亡的小分子药物,并在小鼠耳蜗外植体和斑马鱼侧线毛细胞中进行验证。方法通过Cell Counting Kit-8(CCK8)计算细胞存活率来筛选出对新霉素导致的HEI-OC1细胞系死亡有保护作用的小分子药物;通过免疫荧光染色的方法在小鼠耳蜗外植体和斑马鱼侧线毛细胞中进行验证。结果在筛选的350种小分子药物发现能显著提高新霉素处理的HEI-OC1的细胞中存活率的药物—盐酸氯米帕明;在耳蜗外植体及斑马鱼侧线毛细胞中发现盐酸氯米帕明对新霉素导致的毛细胞死亡有保护作用。结论盐酸氯米帕明作为FDA批准的临床用药一定程度缓解了新霉素导致的毛细胞死亡,说明其对新霉素导致的毛细胞死亡有一定的保护作用。

关键词: 新霉素, 氨基糖苷类, 毛细胞, 盐酸氯米帕明, 耳聋

Abstract: Objective The aim of this study was to identify small-molecule drugs that inhibit neomycin-induced hair-cell-like cell(HEI-OC1)death, and to verify their effects on hair cells in cochlear explants and the lateral line of zebrafish. Methods Small-molecule drugs that increased the viability of HEI-OC1 cells were identified using Cell Counting Kit-8. The protective effect of the identified small-molecule drugs was verified in hair cells of cochlear explants and zebrafish by Immunofluorescence staining. Results Of the 350 small-molecule drugs screened, clomipramine hydrochloride was found to significantly improve the survival rate of neomycin-treated HEI-OC1 cells. In addition, clomipramine hydrochloride was found to have a protective effect on hair cells of neomycin-treated mouse cochlear explants and the lateral line of zebrafish. Conclusion Clomipramine hydrochloride, which as an FDA-approved clinical drug, has a protective effect against neomycin-induced hair cell damage.

Key words: Neomycin, Aminoglycosides, Hair cells, Clomipramine hydrochloride, Deafness

中图分类号:

R764.43

周静,毕秀丽,肖雨,胡俊,付小龙,于亚峰. 盐酸氯米帕明保护听觉毛细胞免受新霉素诱导的损伤[J]. 山东大学耳鼻喉眼学报, 2024, 38(4): 22-27.

ZHOU Jing, BI Xiuli, XIAO Yu, HU Jun, FU Xiaolong, YU Yafeng. Clomipramine hydrochloride protects auditory hair cells from neomycin-induced damage[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2024, 38(4): 22-27.

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