山东大学耳鼻喉眼学报 ›› 2019, Vol. 33 ›› Issue (5): 69-72.doi: 10.6040/j.issn.1673-3770.0.2018.425

• 论著 • 上一篇    下一篇

长链非编码RNA在鼻咽癌组织中表达及其生物学功能研究

李林1,张衡2,冼宇飞3,盛晓丽3()   

  1. 1. 广东省人民医院珠海医院 珠海市金湾中心医院耳鼻喉头颈外科, 广东 珠海 519040
    2. 广州医科大学附属第一医院病理科，广东 广州 510080
    3. 广东省医学科学院 广东省人民医院耳鼻咽喉头颈外科，广东 广州 510080
  • 收稿日期:2018-09-26 修回日期:2018-11-08 出版日期:2019-09-20 发布日期:2019-10-15
  • 通讯作者: 盛晓丽 E-mail:shengzhang2016@126.com

Expression and biological function of long chain non-coding cytoskeleton regulator RNA in nasopharyngeal carcinoma

Lin LI1,Heng ZHANG2,Yufei XIAN3,Xiaoli SHENG3()   

  1. 1. Department of Otorhinolaryngology Head and Neck Surgery, Zhuhai Hospital of Guangdong Provincial People's Hospital，Jinwan Central Hospital, Zhuhai 519040, Guangdong, China
    2. Department of Pathology, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510080, Guangdong, China
    3. Department of Otorhinolaryngology Head and Neck Surgery, Guangdong Academy of Medical Sciences, Guangdong Province People's Hospital, Guangzhou 510080, Guangdong, China
  • Received:2018-09-26 Revised:2018-11-08 Online:2019-09-20 Published:2019-10-15
  • Contact: Xiaoli SHENG E-mail:shengzhang2016@126.com

RichHTML

21

PDF (PC)

141

摘要： 目的

探讨长链非编码RNA (cytoskeleton regulator RNA, CYTOR)在鼻咽癌组织中表达及其生物学功能。

方法

实时荧光定量PCR检测32例鼻咽癌组织及28例鼻咽部良性病变患者黏膜组织中CYTOR的表达情况；Real-time PCR检测人鼻咽上皮细胞（NP69）和鼻咽癌细胞(CNE1、HONE1)中CYTOR的表达情况；应用小干扰RNA抑制CNE1、 HONE1细胞中CYTOR中的表达，并采用MTT、 Transwell 和划痕实验分别检测下调CYTOR表达后鼻咽癌细胞(CNE1， HONE1)的增殖能力、迁移能力变化情况。

 结果

CYTOR在鼻咽癌组织及鼻咽癌细胞系（CNE1、HONE1）中表达水平高于鼻咽部良性病变患者黏膜组织和人鼻咽上皮细胞（NP69），而瞬时抑制CYTOR表达，鼻咽癌细胞系（CNE1、HONE1）的增殖与迁移能力降低，差异均有统计学意义(P <0.05)。

 结论

CYTOR在鼻咽癌组织及鼻咽癌细胞系中高表达，并与鼻咽癌的增殖和迁移能力有关。

关键词: 细胞骨架调节RNA, 鼻咽癌, 侵袭, 转移

Abstract: Objective

To investigate the expression and biological function of long non-coding cytoskeleton regulator RNA (CYTOR) in nasopharyngeal carcinoma.

Methods

Real-time PCR was used to examine CYTOR expression in nasopharyngeal carcinoma tissues (n = 32), nasopharyngeal carcinoma cell lines (CNE1 and HONE1), 28 patients with benign nasopharyngeal lesions, and human nasopharyngeal epithelial cells (NP69). Moreover, CYTOR expression in the CNE1 and HONE1 cell lines was knocked down using RNAi assay. MTT, Transwell, and wound healing assays were also used to detect the proliferation and migration of nasopharyngeal carcinoma cells from CNE1 and HONE1 after downregulating CYTOR expression.

 Results

CYTOR expression in the nasopharyngeal carcinoma tissues and nasopharyngeal carcinoma cell lines was observed to be significantly higher than that in the nasopharyngeal benign lesion mucosal tissues and NP69. After transient inhibition of CYTOR expression, the migration and proliferation abilities of the nasopharyngeal carcinoma cell lines were also observed to decrease significantly (P < 0.05).

 Conclusion

CYTOR expression is elevated in pharyngeal cancer tissues and nasopharyngeal carcinoma cell lines and is positively correlated with the migration ability of nasopharyngeal carcinoma.

Key words: cytoskeleton regulator RNA, Nasopharyngeal Carcinoma, Invasion, Metastasis

中图分类号: 

  • R739.6

表1

下调CYTOR表达抑制鼻咽癌细胞的迁移能力"

时间 组别 宽度 F P
0 h 0.22 0.81
CNE1 406.32±22.11
CNE1_SiNC 411.36±19.21
CNE1_SiCYTOR 414.21±26.06
24 h 46.69 <0.001
CNE1 102.11±26.21
CNE1_SiNC 116.65±30.14
CNE1_SiCYTOR 336.45±45.69

图1

抑制CYTOR表达对鼻咽癌细胞侵袭能力的影响"

1 Wahlestedt C . Targeting long non-coding RNA to therapeutically upregulate gene expression[J]. Nat Rev Drug Discov, 2013, 12(6): 433-446. doi:10.1038/nrd4018 .
doi: 10.1038/nrd4018
2 Zhang H , Chen ZH , Wang XX , et al . Long non-coding RNA: a new player in cancer[J]. J Hematol Oncol, 2013, 6: 37. doi:10.1186/1756-8722-6-37 .
doi: 10.1186/1756-8722-6-37
3 Guttman M , Rinn JL . Modular regulatory principles of large non-coding RNAs[J]. Nature, 2012, 482(7385): 339-346. doi:10.1038/nature10887 .
doi: 10.1038/nature10887
4 Wierzbicki AT . The role of long non-coding RNA in transcriptional gene silencing[J]. Curr Opin Plant Biol, 2012, 15(5): 517-522. doi:10.1016/j.pbi.2012.08.008 .
doi: 10.1016/j.pbi.2012.08.008
5 Liang JY , Wei X , Liu ZH , et al . Long noncoding RNA CYTOR in cancer: a TCGA data review[J]. Clin Chim Acta, 2018, 483: 227-233. doi:10.1016/j.cca. 2018. 05. 010 .
doi: 10.1016/j.cca. 2018. 05. 010
6 Yue B , Liu CC , Sun HM , et al . A positive feed-forward loop between LncRNA-CYTOR and wnt/β-catenin signaling promotes metastasis of colon cancer[J]. Mol Ther, 2018, 26(5): 1287-1298. doi:10.1016/j.ymthe.2018. 02. 024 .
doi: 10.1016/j.ymthe.2018. 02. 024
7 Liongue C , Taznin T , Ward AC . Signaling via the CytoR/JAK/STAT/SOCS pathway: Emergence during evolution[J]. Mol Immunol, 2016, 71: 166-175. doi:10.1016/j. molimm.2016.02.002 .
doi: 10.1016/j. molimm.2016.02.002
8 Wu JL , Shuang ZY , Zhao JF , et al . Linc00152 promotes tumorigenesis by regulating DNMTs in triple-negative breast cancer[J]. Biomed Pharmacother, 2018, 97: 1275-1281. doi:10.1016/j.biopha.2017.11.055 .
doi: 10.1016/j.biopha.2017.11.055
9 Kamran SC , Riaz N , Lee N . Nasopharyngeal carcinoma[J]. Surg Oncol Clin N Am, 2015, 24(3): 547-561. doi:10.1016/j.soc.2015.03.008 .
doi: 10.1016/j.soc.2015.03.008
10 陆海军, 刘霁, 丁晓 . 鼻咽癌的综合治疗研究进展[J]. 山东大学耳鼻喉眼学报, 2019, 33(2): 26-30. doi: 10.6040/j.issn.1673-3770.1.2019.010 .
doi: 10.6040/j.issn.1673-3770.1.2019.010
LU Haijun , LIU Ji , DING Xiao . Progress in the comprehensive treatment for nasopharyngeal carcinoma[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(2): 26-30. doi: 10.6040/j.issn.1673-3770.1.2019.010 .
doi: 10.6040/j.issn.1673-3770.1.2019.010
11 陈瑞, 李杰恩 . 鼻咽癌放疗后诱发头颈部恶性肿瘤的治疗[J]. 山东大学耳鼻喉眼学报, 2019, 33(2): 31-34. doi:10.6040/j.issn.1673-3770.0.2019.072 .
doi: 10.6040/j.issn. 1673-3770.0.2019.072
CHEN Rui , LI Jie′en . Treatment of radiation-induced carcinoma in patients with nasopharyngeal carcinoma[J]. Journal of Otolaryngology and Ophthalmology of Shandong University, 2019, 33(2): 31-34. doi:10.6040/j.issn. 1673-3770.0.2019.072 .
doi: 10.6040/j.issn. 1673-3770.0.2019.072
12 Deniz E , Erman BT . Long noncoding RNA (lincRNA), a new paradigm in gene expression control[J]. Funct Integr Genomics, 2017, 17(2/3): 135-143. doi:10. 1007/s10142-016-0524-x .
doi: 10. 1007/s10142-016-0524-x
13 Sheng L , Ye L , Zhang D , et al . New insights into the long non-coding RNA SRA: physiological functions and mechanisms of action[J]. Front Med (Lausanne), 2018, 5: 244. doi:10.3389/fmed.2018.00244 .
doi: 10.3389/fmed.2018.00244
14 Zampetaki A , Albrecht A , Steinhofel K . Long non-coding RNA structure and function: is there a link?[J]. Front Physiol, 2018, 9: 1201. doi:10.3389/fphys. 2018. 01201 .
doi: 10.3389/fphys. 2018. 01201
15 Bian ZH , Zhang JW , Li M , et al . Correction: Long non-coding RNA LINC00152 promotes cell proliferation, metastasis, and confers 5-FU resistance in colorectal cancer by inhibiting miR-139-5p[J]. Oncogenesis, 2018, 7(8): 63. doi:10.1038/s41389-018-0067-1 .
doi: 10.1038/s41389-018-0067-1
16 Chen PX , Fang XL , Xia B , et al . Long noncoding RNA LINC00152 promotes cell proliferation through competitively binding endogenous miR-125b with MCL-1 by regulating mitochondrial apoptosis pathways in ovarian cancer[J]. Cancer Med, 2018, 7(9): 4530-4541. doi:10.1002/cam4.1547 .
doi: 10.1002/cam4.1547
17 Hu XL , Wang J , He W , et al . Down-regulation of lncRNA Linc00152 suppressed cell viability, invasion, migration, and epithelial to mesenchymal transition, and reversed chemo-resistance in breast cancer cells[J]. Eur Rev Med Pharmacol Sci, 2018, 22(10): 3074-3084. doi:10.26355/eurrev_201805_15067 .
doi: 10.26355/eurrev_201805_15067
18 Cai Q , Wang ZQ , Wang SH , et al . Long non-coding RNA LINC00152 promotes gallbladder cancer metastasis and epithelial-mesenchymal transition by regulating HIF-1α via miR-138[J]. Open Biol, 2017, 7(1): 160247. doi:10.1098/rsob.160247 .
doi: 10.1098/rsob.160247
[1] 潘晓菲,王军,肖洋,马丽晶. LncRNA CTB-147C22.8对复发性呼吸道乳头状瘤细胞侵袭的影响[J]. 山东大学耳鼻喉眼学报, 2019, 33(4): 66-70.
[2] 高晓倩,姜震,耿琛琛,刘大昱,李荔. 术前超声评估分化型甲状腺癌颈部淋巴结转移[J]. 山东大学耳鼻喉眼学报, 2019, 33(1): 135-139.
[3] 许莉,王志颐,陈伟,薛飞,张勇,程友,季俊峰,崔鹏程,江满杰. MicroRNA-24对人喉鳞癌细胞生物学行为的影响[J]. 山东大学耳鼻喉眼学报, 2018, 32(6): 30-37.
[4] 何健,钱立庭,高劲. 外耳道腺样囊性癌侵犯中耳、颞叶且伴颈部淋巴结转移一例报告[J]. 山东大学耳鼻喉眼学报, 2018, 32(6): 109-111.
[5] 李国彬,张占成,王新颜. 上调miR-200b对人喉癌Hep-2细胞增殖、迁移和侵袭能力的影响[J]. 山东大学耳鼻喉眼学报, 2018, 32(4): 53-57.
[6] 赵小燕,吴彩琴,任晓勇,王正辉. 白花蛇舌草多糖提取物诱导Hep-2细胞凋亡与抑制侵袭机制[J]. 山东大学耳鼻喉眼学报, 2018, 32(2): 84-87.
[7] 张彩霞,刘阳云,江文,刘庚勋,曹杭,陈琼,张纪帅. 一氧化氮对鼻咽癌CNE-2细胞株化疗增敏效应的研究[J]. 山东大学耳鼻喉眼学报, 2017, 31(5): 72-78.
[8] 周毅波,龚小蓉,于锋. MiR-150调控Nanog对鼻咽癌侧群细胞增殖、侵袭的影响[J]. 山东大学耳鼻喉眼学报, 2017, 31(5): 79-84.
[9] 朱晓城,钱晓云,顾亚军,沈晓辉,宋盼盼,李惠,高下. MGMT和EGFR蛋白在喉鳞状细胞癌中的表达及临床意义[J]. 山东大学耳鼻喉眼学报, 2017, 31(4): 68-72.
[10] 陈婷婷,郭其云,刘佳琪,刘丽庭. 探讨同期放化疗对儿童青少年鼻咽癌患者生活质量的影响[J]. 山东大学耳鼻喉眼学报, 2017, 31(2): 55-58.
[11] 于斌,郑青平,罗展雄,李旌,周媛媛,李中华,王磊黎,贺婵娟,石丰榕. 重组人表皮生长因子预防鼻咽癌放射性口腔黏膜炎和急性放射性皮炎的疗效观察[J]. 山东大学耳鼻喉眼学报, 2017, 31(1): 95-99.
[12] 王雅宁,耿博,李百彦,崔朝阳,王启荣. 鼻咽癌放疗后颅底骨感染坏死的治疗体会[J]. 山东大学耳鼻喉眼学报, 2016, 30(6): 42-45.
[13] 余济春. 分化型甲状腺癌颈淋巴结清扫术[J]. 山东大学耳鼻喉眼学报, 2016, 30(2): 10-12.
[14] 王君影,李晓峰. 小牛血去蛋白提取物眼用凝胶对鼻咽癌放射治疗后眼角膜内皮细胞损伤的影响[J]. 山东大学耳鼻喉眼学报, 2016, 30(1): 76-79.
[15] 江昌,许承弼,辛丁,鞠洋,李鸿燕,王金月. 下咽癌腋窝淋巴结转移1例及文献回顾[J]. 山东大学耳鼻喉眼学报, 2016, 30(1): 90-92.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
网站版权所有 © 2018 《山东大学耳鼻喉眼学报》编辑部　
地址：山东省济南市山大南路27号（250100）电话：+86-0531-88366912 E-mail: ebhxb@sdu.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发